DDAO galactoside (9<i>H</i>-(1,3-Dichloro-9,9-Dimethylacridin-2-One-7-yl) β-D-Galactopyranoside)

Citations & References (5)

Invitrogen™

DDAO galactoside (9H-(1,3-Dichloro-9,9-Dimethylacridin-2-One-7-yl) β-D-Galactopyranoside)

The galactosidase substrate, DDAO galactoside yields a hydrolysis product that can be excited with the 633 nm laser (excitation/emission maxima ∼645/660).

Catalog Number	Quantity
D6488 also known as D-6488	5 mg

Catalog number D6488

also known as D-6488

Price (INR)

Quantity:

The galactosidase substrate, DDAO galactoside yields a hydrolysis product that can be excited with the 633 nm laser (excitation/emission maxima ∼645/660). Although the substrate itself is fluorescent (excitation/emission maxima ∼460/610 nm), the difference between the substrate's and the hydrolysis product is over 200 nm, along the two species to be easily distinguished.

To make a concentrated stock solution of up to 5 mg/mL, dissolve in high quality anhydrous DMSO. Store frozen, desiccated and protected from light.

For Research Use Only. Not for use in diagnostic procedures.

Specifications

Excitation/Emission465/608 nm

Molecular Weight (g/mol)470.31

Quantity5 mg

Shipping ConditionRoom Temperature

SubstrateBeta-Gal Substrate

Detection MethodFluorescence

Substrate PropertiesChemical Substrate

Unit SizeEach

Contents & Storage

Store in freezer (-5°C to -30°C) and protect from light.

Citations & References (5)

Citations & References

Abstract

Oxygen sensitivity of reporter genes: implications for preclinical imaging of tumor hypoxia.

Authors:Cecic I, Chan DA, Sutphin PD, Ray P, Gambhir SS, Giaccia AJ, Graves EE

Journal:Mol Imaging

PubMed ID:17711777

'Reporter gene techniques have been applied toward studying the physiologic phenomena associated with tumor hypoxia, a negative prognostic indicator. The purpose of this study was to assess the potential adverse effects of hypoxic conditions on the effectiveness of four commonly used reporter genes: Renilla luciferase, monomeric red fluorescent protein, thymidine ... More

In vivo imaging of beta-galactosidase activity using far red fluorescent switch.

Authors:Tung CH, Zeng Q, Shah K, Kim DE, Schellingerhout D, Weissleder R

Journal:Cancer Res

PubMed ID:14996712

'beta-Galactosidase (beta-gal) has been widely used as a transgene reporter enzyme, and several substrates are available for its in vitro detection. The ability to image beta-gal expression in living animals would further extend the use of this reporter. Here we show that DDAOG, a conjugate of beta-galactoside and 7-hydroxy-9H-(1,3-dichloro-9,9-dimethylacridin-2-one) (DDAO), ... More

Enzymatic detection of protein translocation.

Authors:Wehrman TS, Casipit CL, Gewertz NM, Blau HM

Journal:Nat Methods

PubMed ID:15973423

Fundamental to eukaryotic cell signaling is the regulation of protein function by directed localization. Detection of these events has been largely qualitative owing to the limitations of existing technologies. Here we describe a method for quantitatively assessing protein translocation using proximity-induced enzyme complementation. The complementation assay for protein translocation (CAPT) ... More

Identification of a Sacral, Visceral Sensory Transcriptome in Embryonic and Adult Mice.

Authors:

Journal:Eneuro

PubMed ID:31996391

Modulation of therapy-induced senescence by reactive lipid aldehydes.

Authors:Flor AC, Doshi AP, Kron SJ,

Journal:Cell Death Discov

PubMed ID:27453792

Current understanding points to unrepairable chromosomal damage as the critical determinant of accelerated senescence in cancer cells treated with radiation or chemotherapy. Nonetheless, the potent senescence inducer etoposide not only targets topoisomerase II to induce DNA damage but also produces abundant free radicals, increasing cellular reactive oxygen species (ROS). Toward ... More