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Citations & References (10)
Invitrogen™
EnzChek™ Pyrophosphate Assay Kit
The EnzChek™ Pyrophosphate Assay Kit provides a rapid and highly sensitive enzymatic assay for detecting free pyrophosphate in solution. InRead more
| Catalog Number | Quantity |
|---|---|
| E6645 | 100 Assays |
Catalog number E6645
Price (INR)Request A Quote
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Quantity:
100 Assays
The EnzChek™ Pyrophosphate Assay Kit provides a rapid and highly sensitive enzymatic assay for detecting free pyrophosphate in solution. In this assay the formation of a chromophoric product is detected spectrophotometrically.
See our complete line of Fluorescence Microplate assays.
• Sensitivity limit of 1 μM pyrophosphate (∼0.2 μg/mL)
• Acommodates a pH range of 6.5 to 8.5
The EnzChek™ Pyrophosphate Detection Kit provides a fast, convenient, and inexpensive spectrophotometric method for measuring the inorganic pyrophosphate produced by a number of biochemical reactions, such as DNA and RNA polymerizations, cyclic AMP formation by the enzyme adenylate cyclase, and the enzymatic activation of fatty acids to form their coenzyme A esters.
The EnzChek™ Pyrophosphate Assay Kit is a modification of our EnzChek™ Phosphate Assay Kit (Cat. No. E6646). In the presence of inorganic phosphate, the substrate 2-amino-6-mercapto-7-methylpurine ribonucleoside (MESG) is converted by the purine nucleoside phosphorylase (PNP) enzyme to ribose 1-phosphate and 2-amino-6-merc apto-7-methylpurine. The enzymatic conversion of MESG results in a shift in absorbance maximum from 330 nm to 360 nm. The EnzChek™ Pyrophosphate Assay Kit includes the enzyme inorganic pyrophosphatase, which catalyzes conversion of pyrophosphate into two equivalents of phosphate. The phosphate is then consumed by the MESG/PNP reaction and detected by an increase in absorbance at 360 nm. Additional sensitivity is gained by the amplification of one molecule of pyrophosphate into two molecules of phosphate.
See our complete line of Fluorescence Microplate assays.
• Sensitivity limit of 1 μM pyrophosphate (∼0.2 μg/mL)
• Acommodates a pH range of 6.5 to 8.5
The EnzChek™ Pyrophosphate Detection Kit provides a fast, convenient, and inexpensive spectrophotometric method for measuring the inorganic pyrophosphate produced by a number of biochemical reactions, such as DNA and RNA polymerizations, cyclic AMP formation by the enzyme adenylate cyclase, and the enzymatic activation of fatty acids to form their coenzyme A esters.
The EnzChek™ Pyrophosphate Assay Kit is a modification of our EnzChek™ Phosphate Assay Kit (Cat. No. E6646). In the presence of inorganic phosphate, the substrate 2-amino-6-mercapto-7-methylpurine ribonucleoside (MESG) is converted by the purine nucleoside phosphorylase (PNP) enzyme to ribose 1-phosphate and 2-amino-6-merc apto-7-methylpurine. The enzymatic conversion of MESG results in a shift in absorbance maximum from 330 nm to 360 nm. The EnzChek™ Pyrophosphate Assay Kit includes the enzyme inorganic pyrophosphatase, which catalyzes conversion of pyrophosphate into two equivalents of phosphate. The phosphate is then consumed by the MESG/PNP reaction and detected by an increase in absorbance at 360 nm. Additional sensitivity is gained by the amplification of one molecule of pyrophosphate into two molecules of phosphate.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Detection MethodColorimetric
Dye TypeMESG
FormatTube(s)
Quantity100 Assays
Shipping ConditionRoom Temperature
ColorUltraviolet
For Use With (Application)Pyrophosphate Assay
For Use With (Equipment)Spectrophotometer
Product LineEnzChek
Product TypeEnzChek Assay Kit
Unit SizeEach
Contents & Storage
Includes inorganic pyrophosphatase, 2-Amino-6-mercapto-7-methylpurine riboside (MESG), purine nucleoside phosphorylase (PNP), concentrated reaction buffer, Na2P2O7 standard, and detailed protocols for detecting and quantitating pyrophosphate.
Frequently asked questions (FAQs)
What is the sensitivity limit of the EnzCheck Pyrophosphate Assay kit?
Citations & References (10)
Citations & References
Abstract
Presence of a plant-like proton-pumping pyrophosphatase in acidocalcisomes of Trypanosoma cruzi.
Journal:J Biol Chem
PubMed ID:9705361
'The vacuolar-type proton-translocating pyrophosphatase (V-H+-PPase) is an enzyme previously described in detail only in plants. This paper demonstrates its presence in the trypanosomatid Trypanosoma cruzi. Pyrophosphate promoted organellar acidification in permeabilized amastigotes, epimastigotes, and trypomastigotes of T. cruzi. This activity was stimulated by K+ ions and was inhibited by Na+
A broadly applicable continuous spectrophotometric assay for measuring aminoacyl-tRNA synthetase activity.
Journal:Nucleic Acids Res
PubMed ID:7659511
'We describe a convenient, simple and novel continuous spectrophotometric method for the determination of aminoacyl-tRNA synthetase activity. The assay relies upon the measurement of inorganic pyrophosphate generated in the first step of the aminoacylation of a tRNA. Pyrophosphate release is coupled to inorganic pyrophosphatase, to generate phosphate, which in turn
Thiamin biosynthesis in Escherichia coli. Identification of this thiocarboxylate as the immediate sulfur donor in the thiazole formation.
Journal:J Biol Chem
PubMed ID:9632726
ThiFSGH and ThiI are required for the biosynthesis of the thiazole moiety of thiamin in Escherichia coli. The overproduction, purification, and characterization of ThiFS and the identification of two of the early steps in the biosynthesis of the thiazole moiety of thiamin are described here. ThiS isolated from E. coli
AcsD catalyzes enantioselective citrate desymmetrization in siderophore biosynthesis.
Journal:Nat Chem Biol
PubMed ID:19182782
Bacterial pathogens need to scavenge iron from their host for growth and proliferation during infection. They have evolved several strategies to do this, one being the biosynthesis and excretion of small, high-affinity iron chelators known as siderophores. The biosynthesis of siderophores is an important area of study, not only for
A spectrophotometric method to measure enzymatic activity in reactions that generate inorganic pyrophosphate.
Journal:Anal Biochem
PubMed ID:8954523
This paper describes a spectrophotometric assay that can measure the inorganic pyrophosphate produced from various enzymatic reactions. This is a coupled assay in which the addition of inorganic pyrophosphatase initially cleaves the pyrophosphate into two molecules of phosphate. The phosphate is then detected by the conversion of 2-amino-6-mercapto-7-methylpurine ribonucleoside to