Total Antioxidant Capacity (T-AOC) Colorimetric Assay Kit

The Total Antioxidant Capacity (T-AOC) Colorimetric Assay Kit is a highly reliable and sensitive assay designed for the quantitative measurement of T-AOC in serum, plasma, whole blood, tissue, cells, and culture supernatant. T-AOC refers to the overall ability of a biological sample to counteract oxidative stress by neutralizing reactive oxygen species (ROS) and other harmful free radicals. It represents the cumulative effect of both enzymatic and non-enzymatic antioxidants present in the sample.

The assay quantifies the ability of the sample to reduce ferric ions (Fe³⁺) to ferrous ions (Fe²⁺) using a phenanthroline substance. The resulting complex exhibits a characteristic absorbance at 520 nm, which is directly proportional to the T-AOC of the sample.

Key features

• Simple and fast protocol – user-friendly, requiring minimal hands-on time and providing rapid results within a short incubation period
• Broad range of kits – across key analytes and targets within the most important metabolic pathways
• Consistent – strict quality control criteria to ensure reproducible and reliable results