Fura-4F, AM, cell permeant - Special Packaging

Citations & References (14)

Invitrogen™

Fura-4F, AM, cell permeant - Special Packaging

Fura-4F AM is an intracellular calcium indicator similar to fura-2 but with a response range shifted to detect higher Ca2+Read more

Catalog Number	Quantity
F14175	10 x 50 μg

Catalog number F14175

Price (INR)

62,005.00

Add to cart

Quantity:

10 x 50 μg

Price (INR)

62,005.00

Add to cart

Fura-4F AM is an intracellular calcium indicator similar to fura-2 but with a response range shifted to detect higher Ca²⁺ concentrations. This acetoxymethyl (AM) ester derivative is useful for noninvasive intracellular loading. This sku can be used in place of the recently discontinued fura 5F AM, F14177. Ex/Ems are identical, and more importantly the Kd's(Ca²⁺) μM are very similar: 0.40 for fura-5F AM vs 0.77 for fura-4F AM.

Learn more about ion indicators including calcium, potassium, pH, and membrane potential indicators ›

For Research Use Only. Not for use in diagnostic procedures.

Specifications

Detection MethodFluorescence

Dye TypeFluorescent Dye-Based

Quantity10 x 50 μg

Shipping ConditionRoom Temperature

For Use With (Application)Cell Viability and Proliferation

For Use With (Equipment)Fluorescence Microscope

Product TypeDye

Unit SizeEach

Contents & Storage

Store in freezer -5°C to -30°C and protect from light.

Frequently asked questions (FAQs)

I am doing calcium flux imaging with your Fura-2 calibration kit, but am seeing a large variability in ratio in different places around the slide. I am correcting for uniform illumination, using the product as directed, and sealing the coverslip with nail polish.

The nail polish may be the problem. The K_d value (calcium sensitivity) changes depending upon the dye's environment. Nail polish has solvents that can leech under the coverslip and cause variability. We recommend either going without a sealing or sealing with melted paraffin painted on the coverslip edges with a cotton-tipped applicator (paraffin is hydrophobic and has no solvents).

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

I need to label cells with Fluo-4, AM, for a calcium flux assay. How long after labeling will the dye be retained?

After loading dye into the cells, intracellular esterases remove the 'AM' moiety from the dye. When the 'AM' group is removed, the dye is able to bind calcium and fluoresce. Since the dye is not covalently bound to any cellular components, it may be actively effluxed from the cell. The rate of efflux is dependent upon the inherent properties of the cell, culture conditions and other factors. The dye may be retained for hours, days or even weeks or lost in a matter of minutes. The use of Probenecid (Cat. No. P36400) limits loss by active efflux.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Citations & References (14)

Citations & References

Abstract

Modulation of plasma membrane calcium-ATPase activity by local calcium microdomains near CRAC channels in human T cells.

Authors:Bautista DM, Lewis RS

Journal:J Physiol

PubMed ID:14966303

'The spatial distribution of Ca(2+) signalling molecules is critical for establishing specific interactions that control Ca(2+) signal generation and transduction. In many cells, close physical coupling of Ca(2+) channels and their targets enables precise and robust activation of effector molecules through local [Ca(2+)](i) elevation in microdomains. In T cells, the ... More

Examining synaptotagmin 1 function in dense core vesicle exocytosis under direct control of Ca2+.

Authors:Sørensen JB, Fernández-Chacón R, Südhof TC, Neher E

Journal:J Gen Physiol

PubMed ID:12939392

'We tested the long-standing hypothesis that synaptotagmin 1 is the Ca2+ sensor for fast neurosecretion by analyzing the intracellular Ca2+ dependence of large dense-core vesicle exocytosis in a mouse strain carrying a mutated synaptotagmin C2A domain. The mutation (R233Q) causes a twofold increase in the KD of Ca2+-dependent phospholipid binding ... More

Hyperinsulinism induced by targeted suppression of beta cell KATP channels.

Authors:Koster JC, Remedi MS, Flagg TP, Johnson JD, Markova KP, Marshall BA, Nichols CG

Journal:Proc Natl Acad Sci U S A

PubMed ID:12486236

'ATP-sensitive K+ (K(ATP)) channels couple cell metabolism to electrical activity. To probe the role of K(ATP) in glucose-induced insulin secretion, we have generated transgenic mice expressing a dominant-negative, GFP-tagged K(ATP) channel subunit in which residues 132-134 (Gly-Tyr-Gly) in the selectivity filter were replaced by Ala-Ala-Ala, under control of the insulin ... More

Rab3A negatively regulates activity-dependent modulation of exocytosis in bovine adrenal chromaffin cells.

Authors:Thiagarajan R, Tewolde T, Li Y, Becker PL, Rich MM, Engisch KL

Journal:J Physiol

PubMed ID:14694148

'Members of the Rab family of monomeric GTPases have been implicated in vesicle trafficking, and Rab3A, located on synaptic vesicles in neurones and secretory vesicles in neuroendocrine cells, is likely to be involved in vesicle fusion leading to neurotransmitter release. A hydrolysis-deficient mutant of Rab3A, Rab3AQ81L, has been shown to ... More

Abnormal intracellular calcium homeostasis in sympathetic neurons from young prehypertensive rats.

Authors:Li D, Lee CW, Buckler K, Parekh A, Herring N, Paterson DJ,

Journal:Hypertension

PubMed ID:22252398

'Hypertension is associated with cardiac noradrenergic hyperactivity, although it is not clear whether this precedes or follows the development of hypertension itself. We hypothesized that Ca(2+) homeostasis in postganglionic sympathetic neurons is impaired in spontaneously hypertensive rats (SHRs) and may occur before the development of hypertension. The depolarization-induced rise in ... More

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