Phire Plant Direct PCR Master Mix
Phire Plant Direct PCR Master Mix
Thermo Scientific™

Phire Plant Direct PCR Master Mix

Thermo Scientific Phire Plant Direct PCR Master Mix is designed to amplify DNA directly from plant samples.
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Catalog NumberNo. of Reactions
F160L1250 Reactions
F160S250 Reactions
Catalog number F160L
Price (INR)
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No. of Reactions:
1250 Reactions
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Thermo Scientific Phire Plant Direct PCR Master Mix is designed to amplify DNA directly from plant samples. No DNA purification is required prior to PCR. The kit is based on Phire Hot Start II DNA Polymerase, a specially engineered enzyme with a DNA binding domain. The unique features of this DNA polymerase make it extremely robust and tolerant of many PCR inhibitors present in plant material like complex polysaccharides, polyphenols, and others.

Phire Plant Direct PCR Master Mix has been tested with leaves and seeds from a wide variety of plant species as well as fungi, bacteria, and yeast. The kit includes a complete set of optimized reagents and detailed protocols, making it an ideal choice for amplification of plant DNA.

Features

  • Direct PCR allows sample to be added directly to PCR reaction, reducing time-consuming and expensive DNA purification steps
  • Available Dilution & Storage protocol allows several PCR reactions from one sample
  • Specially engineered Phire Hot Start II DNA Polymerase for extremely short PCR protocol times
  • Master mix format with premixed gel loading dye minimizes risk of cross-contamination, reduces sample handling, and allows direct loading on gel

Applications

  • Genotyping
  • Transgene detection
  • Knockout analysis
  • Sequencing

Notes

  • Annealing temperature for Phire DNA Polymerases may differ significantly from that of Taq-based polymerases.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Hot StartBuilt-In Hot Start
No. of Reactions1250 Reactions
OverhangBlunt
PolymerasePhire Hot Start II DNA Polymerase
Product TypeDirect PCR Master Mix
Quantity1,250 reactions
Reaction FormatSuperMix or Master Mix
Sample TypePlant
Shipping ConditionWet or Dry Ice
Starting MaterialDNA
Concentration2X
For Use With (Application)Genotyping, Hot-start PCR
GC-Rich PCR PerformanceMedium
PCR MethodDirect PCR
Reaction SpeedFast
Unit SizeEach
Contents & Storage
• 2X Phire Plant Direct PCR Master Mix, 10 x 1.25 mL
• Dilution Buffer, 2 x 5 mL
• 25 μM Control Primer Mix, 40 μL
• Nuclease-free water, 10 x 1.25 mL
• O'GeneRuler Express DNA Ladder, 50 μg (sufficient for 100 applications)

Store at -20°C.

Frequently asked questions (FAQs)

What is your recommendation to take a sample from plant or animal tissue for Direct PCR?

In order to obtain best results from Direct PCR, it is important to use a very small amount of starting materials. The recommended size is 0.3-0.5 mm in diameter. We recommend using Tissue Puncher 0.3 mm (Cat. No. F-200S), which is a convenient tool for sampling and is suitable for both plant and animal tissues.

Why are Control primers provided in the Direct PCR Kits?

It is very important to include a control reaction in the Direct PCR to ensure that the PCR conditions are optimal for the specific tissue or plant material used. Control primers are universal primers that amplify a small fragment from a conserved genome region. If the reaction with control primers fails, the sample material may not be suitable for Direct PCR, or PCR conditions need optimization.

Can I use plant or tissue sample which is not on the list of the Direct PCR Master Mix and Kit tested samples?

You can test any plant or tissue sample. However, it is recommended to first optimize the reaction. When working with new sample materials, start with the Dilution & Storage protocol as it allows several PCR reactions to be performed from the same sample during optimization. It is recommended to perform a positive control with purified sample DNA to ensure that the PCR conditions are working. If the positive control with purified DNA fails, the PCR conditions should be optimized before continuing further.

Can you amplify the same sample materials with the Direct PCR Master Mixes as with Direct PCR Kits?

Yes, the Direct PCR Master Mixes are upgrades of the Direct PCR kits and thus are suitable for the same sample materials.

Do the Direct PCR Master Mixes and Kits come with tissue punchers?

No, tissue punchers are not provided. You can purchase 0.3 mm Tissue Punchers (Cat. No. F-200S), or cut the sample with a scalpel. Some scientists use 100 µL pipette tips to take a sample.

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