FluoSpheres™ Carboxylate-Modified Microspheres
FluoSpheres™ Carboxylate-Modified Microspheres
Invitrogen™

FluoSpheres™ Carboxylate-Modified Microspheres

Achieve the brightest fluorescence with Carboxylate-Modified FluoSphere Microspheres, available in different colors and particle sizes.
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Catalog NumberDiameter (Metric)ColorExcitation/EmissionQuantity
F87840.02 μmNile Red535/575 nm10 mL
F88030.1 μmYellow-Green505/515 nm10 mL
F88161.0 μmCrimson625/645 nm2 mL
F88231.0 μmYellow-Green505/515 nm10 mL
F88010.1 μmRed580/605 nm10 mL
F88110.2 μmYellow-Green505/515 nm10 mL
F88070.2 μmDark Red660/680 nm2 mL
F107200.04 μmYellow-Green, Orange, Red, Dark Red505/515, 540/560, 580/605, 660/680 nm1 mL/each
F208810.2 μmOrange365/610 nm2 mL
F8783
also known as F-8783
0.02 μmDark Red660/680 nm2 mL
F87860.02 μmRed580/605 nm10 mL
F8795
also known as F-8795
0.04 μmYellow-Green505/515 nm1 mL
F88130.5 μmYellow-Green505/515 nm10 mL
F88201.0 μmOrange540/560 nm10 mL
F88252.0 μmNile Red535/575 nm2 mL
F88272.0 μmYellow-Green505/515 nm2 mL
F8781
also known as F-8781
0.02 μmBlue365/415 nm10 mL
F8782
also known as F-8782
0.02 μmCrimson625/645 nm2 mL
F87870.02 μmYellow-Green505/515 nm10 mL
F8789
also known as F-8789
0.04 μmDark Red660/680 nm1 mL
F8792
also known as F-8792
0.04 μmOrange540/560 nm1 mL
F87930.04 μmRed580/605 nm1 mL
F87940.04 μmRed-Orange565/580 nm1 mL
F87970.1 μmBlue350/440 nm10 mL
F87990.1 μmInfrared715/755 nm1 mL
F8800
also known as F-8800
0.1 μmOrange540/560 nm10 mL
F88050.2 μmBlue365/415 nm10 mL
F88060.2 μmCrimson625/645 nm2 mL
F88090.2 μmOrange540/560 nm10 mL
F88100.2 μmRed580/605 nm10 mL
F88120.5 μmRed580/605 nm10 mL
F88141.0 μmBlue365/415 nm10 mL
F88151.0 μmBlue350/440 nm10 mL
F88191.0 μmNile Red535/575 nm10 mL
F88211.0 μmRed580/605 nm10 mL
F8824
also known as F-8824
2.0 μmBlue365/415 nm2 mL
F88262.0 μmRed580/605 nm2 mL
Catalog number F8784
Price (INR)
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Diameter (Metric):
0.02 μm
Color:
Nile Red
Excitation/Emission:
535/575 nm
Quantity:
10 mL

Easily perform flow cytometry, microscopy, HTS, HCS, immunoassay, and other laboratory applications using our extensive selection of FluoSpheres Carboxylate-Modified Microspheres. FluoSphere beads can be used in passive adsorption or active, covalent coupling of proteins, nucleic acids, and biomolecules for particle capture applications. FluoSphere microspheres are loaded with proprietary fluorescent dyes, making them the brightest microspheres available.

Visualize the brightest fluorescence for laboratoy applications including fluorescence microscopy, flow cytometry, HTS, HCS, and cell tracing with our Carboxylate-Modified FluoSphere Microspheres, which are manufactured from polystyrene microspheres and loaded with different proprietary dyes. Using specialized staining methods enables all of the fluorescent dye molecules to be contained inside each polystyrene microsphere instead of on the bead's surface. This protective environment within the bead shields the dye from detrimental environmental effects, such as photobleaching. Our carboxylate-modified microspheres are coated with a hydrophilic polymer containing multiple carboxylic acids for covalent attachment of ligands. A range of particle sizes is available for different research uses and experiments.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Excitation/Emission535/575 nm
Product LineFLUOSPHERES
Quantity10 mL
Surface ModificationCarboxylate
ColorNile Red
Diameter (Metric)0.02 μm
For Use With (Application)Fluorescence Microscopy
MaterialPolystyrene
Product TypeCarboxylate-Modified Microsphere
Unit SizeEach
Contents & Storage
Store in refrigerator (2–8°C) and protect from light.

Frequently asked questions (FAQs)

I have some FluoSpheres polystyrene microspheres, with 20 nm diameter. They are aggregating a lot. What can I do about it?

The smaller the microspheres, the greater the propensity to aggregate. But the aggregation is not irreversible. Sonicate in a bath sonicator or vortex to disperse, just prior to use. You can also add a small concentration of Tween-20 or Triton X-100 (unless you are using them in a live-cell system).

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

I sonicated my 2.0 µm carboxylate-modified microspheres, as recommended, but saw foaming (bubbles) on top of the solution. Should I be concerned?

Use of a bath sonicator is recommended to help break up any aggregated microspheres. The foaming is from Tween-20, which is in the stock solution to help prevent aggregation. It is normal and expected to see bubbles from this. Do not use a probe sonicator, which would cause damage to the microspheres (as well as much more bubbling).

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

What is the warranty for FluoSpheres microspheres?

The warranty period for FluoSpheres microspheres is 1-year from the date of shipment.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

After washing and centrifugation, there was only a very small pellet left of my microsphere beads and the solution was transparent. Why is this?

Centrifugation is not an effective way to collect smaller microspheres; many particles remain in the solution even if you can visualize a small pellet. For beads less than 1 µm in diameter, we recommend washing by either:

Cross-flow filtration, as these particles have a very high compression modulus and can withstand high g-forces without risk of harm or dialysis with a 500 kDa MWCO
Note: Microspheres greater than 1 µm in diameter can be centrifuged at 1,300 rpm.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

I've had my microspheres for over a year, and I'm wondering if they're still good to use. What are some good ways to check their functionality?

Bacterial contamination is the most common cause of microspheres becoming unusable. Many of our particles are supplied with a low level of sodium azide to prevent bacterial contamination, but sometimes this can still occur. Bacterial contamination is best assessed by plating on appropriate growth medium and checking the plates after 72 hr.

Find additional tips, troubleshooting help, and resources within ourMicrospheres Support Center.

Citations & References (2)

Citations & References
Abstract
Microfluidic actuation using electrochemically generated bubbles.
Authors:Hua SZ, Sachs F, Yang DX, Chopra HD
Journal:Anal Chem
PubMed ID:12510764
Bubble-based actuation in microfluidic applications is attractive owing to elementary microfabrication requirements. In the present study, the mechanical and chemical characteristics of electrochemically generated bubble valves were studied. By generating electrochemical bubbles as valves directly inside the channel, valves could be closed and opened in milliseconds. Whereas bubble inflation (or ... More
In situ single-molecule imaging with attoliter detection using objective total internal reflection confocal microscopy.
Authors:Burghardt TP, Ajtai K, Borejdo J
Journal:Biochemistry
PubMed ID:16566579
Confocal microscopy is widely used for acquiring high spatial resolution tissue sample images of interesting fluorescent molecules inside cells. The fluorescent molecules are often tagged proteins participating in a biological function. The high spatial resolution of confocal microscopy compared to wide field imaging comes from an ability to optically isolate ... More