ViraPower™ UbC Lentiviral Gateway™ Expression Kit
Product Image
Invitrogen™

ViraPower™ UbC Lentiviral Gateway™ Expression Kit

The ViraPower™ UbC Lentiviral Gateway™ Expression Kit includes all the components needed to generate lentivirus, including vector kit, 293FT cellRead more
Have Questions?
Catalog NumberQuantity
K4990001 kit
Catalog number K499000
Price (INR)
196,919.00
Online offer
Ends: 31-Mar-2026
328,197.00
Save 131,278.00 (40%)
Each
Add to cart
Quantity:
1 kit
Price (INR)
196,919.00
Online offer
Ends: 31-Mar-2026
328,197.00
Save 131,278.00 (40%)
Each
Add to cart
The ViraPower™ UbC Lentiviral Gateway™ Expression Kit includes all the components needed to generate lentivirus, including vector kit, 293FT cell line, and the support kit. This kit combines Invitrogen™’s ViraPower™ Lentiviral and Gateway™ technologies to facilitate easy recombination-based cloning and lentiviral-based expression of a target gene in dividing and non-dividing mammalian cells. The pLenti6⁄UbC⁄V5-DEST™ vector has the UbC promoter for driving constitutive but physiological levels of expression of the target gene and the blasticidin selection marker for stable selection in mammalian cells.

Advantages
• Stable expression
• Long-term experiments
• Accurate titer of functional virus
• Flexible and versatile Gateway™ recombination cloning technology

Key Features
• UbC promoter
• V5 epitope tag at C terminus
• Blasticidin selection

Kit includes
• pLenti6⁄UbC⁄V5-DEST™ Gateway™ Vector (V499-10)
• ViraPower™ Bsd Lentiviral Support Kit (K4970-00)
• 293FT Cell Line (R700-07)

For research use only. Not intended for any therapeutic or diagnostic use.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Constitutive or Inducible SystemConstitutive
Delivery TypeLentiviral
For Use With (Application)Viral Expression
Product TypeLentiviral Expression Kit
Quantity1 kit
Selection Agent (Eukaryotic)Blasticidin
VectorpLenti
Cloning MethodGateway
Product LineGateway, ViraPower
PromoterUbC
Protein TagV5 Epitope Tag
Unit SizeEach
Contents & Storage
Vector kit (Boxes 1 and 2)
pLenti6⁄UbC⁄V5-DEST™ (Box 1): 6 μg, -20°C
pLenti6⁄UbC⁄V5-GW⁄lacZ Control vector (Box 1): 10 μg, -20°C
One Shot™ Stbl3™ Chemically Competent E. coli (Box 2): 21 x 50 μL, -80°C
pUC19 control DNA (10 pg⁄μL): 50 μL, -80°C
S.O.C. Medium: 6 mL -80°C

Support kit (Box 3)
ViraPower™ Packaging Mix (1 μg⁄μL): 195 μg, -20°C
Lipofectamine™ 2000: 0.75 mL, +4°C
Blasticidin: 50 mg, -20°C
293 FT cells (3 x 106⁄mL) (Box 4): 1 mL, liquid nitrogen

Frequently asked questions (FAQs)

Can I perform the single-step protocol for the BP/LR Clonase reaction using BP Clonase enzyme and LR Clonase enzyme instead of BP Clonase II enzyme and LR Clonase II enzyme?

In the single-step protocol for the BP/LR Clonase reaction, we would not recommend substituting the BP Clonase II/LR Clonase II enzymes with BP Clonase /LR Clonase enzymes as this would result in very low recombination efficiency.

Do you have a recommended single-step protocol for BP/LR recombination?

Yes, we have come up with a single-step protocol for BP/LR Clonase reaction (http://www.thermofisher.com/us/en/home/life-science/cloning/gateway-cloning.html#1), where DNA fragments can be cloned into Destination vectors in a single step reaction, allowing you to save time and money.

How can I move my gene of interest from a Gateway-adapted expression clone to a new Destination vector as I have lost the entry clone?

We would recommend performing a BP reaction with a Donor vector in order to obtain an entry clone. This entry clone can then be used in an LR reaction with the Destination vector to obtain the new expression clone.

Can I purchase the 5X LR Clonase buffer or 5X BP Clonase buffer separately?

We do not offer the 5X LR Clonase buffer and 5X BP Clonase buffer as standalone products. They are available as part of the enzyme kits.

Do you offer Gateway vectors for expression in plants?

We do not offer any Gateway vectors for expression in plants.