Search
Citations & References (64)
Invitrogen™
NBD Cholesterol (22-(N-(7-Nitrobenz-2-Oxa-1,3-Diazol-4-yl)Amino)-23,24-Bisnor-5-Cholen-3β-Ol)
This fluorescent analog, NBD cholesterol, is an environment-sensitive probe that localizes in the membrane's interior and is useful for investigatingRead more
| Catalog Number | Quantity |
|---|---|
| N1148 | 10 mg |
Catalog number N1148
Price (INR)Request A Quote
-
Quantity:
10 mg
This fluorescent analog, NBD cholesterol, is an environment-sensitive probe that localizes in the membrane's interior and is useful for investigating lipid transport processes as well lipid-protein interactions.
We recommend dissolving in chloroform (CHCl3) or acetonitrile (MeCN) to attain a stock concentration up to 1 mM.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Chemical Name or MaterialCholesterol Analogs
Excitation/Emission469/537 nm
Recommended StorageStore at room temperature and protect from light.
Physical FormSolid
Product LineInvitrogen
Quantity10 mg
Unit SizeEach
Citations & References (64)
Citations & References
Abstract
Inactivation of NPC1L1 causes multiple lipid transport defects and protects against diet-induced hypercholesterolemia.
Journal:J Biol Chem
PubMed ID:15671032
'NPC1L1, a recently identified relative of Niemann-Pick C1, was characterized to determine its subcellular location and potential function(s). NPC1L1 was highly expressed in HepG2 cells and localized in a subcellular vesicular compartment rich in the small GTPase Rab5. mRNA expression profiling revealed significant differences between mouse and man with highest
High density lipoprotein-mediated cholesterol uptake and targeting to lipid droplets in intact L-cell fibroblasts. A single- and multiphoton fluorescence approach.
Journal:J Biol Chem
PubMed ID:10777574
'Fluorescent sterols, dehydroergosterol and NBD-cholesterol, were used to examine high density lipoprotein-mediated cholesterol uptake and intracellular targeting in L-cell fibroblasts. The uptake, but not esterification or targeting to lipid droplets, of these sterols differed >100-fold, suggesting significant differences in uptake pathways. NBD-cholesterol uptake kinetics and lipoprotein specificity reflected high density
Lipid binding to sterol carrier protein-2 is inhibited by ethanol.
Journal:Biochim Biophys Acta
PubMed ID:9931423
'Sterol carrier protein-2 (SCP-2) is an intracellular lipid carrier protein that binds cholesterol, phospholipids, fatty acids and other ligands. It has been reported that expression of SCP-2 was increased in brain nerve endings or synaptosomes of chronic ethanol-treated mice and it was shown that cholesterol homeostasis was altered in brain
Formation of membrane domains created during the budding of vesicular stomatitis virus. A model for selective lipid and protein sorting in biological membranes.
Journal:Biochemistry
PubMed ID:7543280
'Vesicular stomatitis virus buds from domains of the plasma membrane that have a unique protein and lipid composition. Fluorescence digital imaging microscopy and resonance energy transfer were used to determine how the two viral envelope-associated proteins, the G and the M proteins, could alter the lateral distribution of lipids in
Membrane properties of D-erythro-N-acyl sphingomyelins and their corresponding dihydro species.
Journal:Biophys J
PubMed ID:11325733
'We have prepared acyl chain-defined D-erythro-sphingomyelins and D-erythro-dihydrosphingomyelins and compared their properties in monolayer and bilayer membranes. Surface pressure/molecular area isotherms of D-erythro-N-16:0-sphingomyelin (16:0-SM) and D-erythro-N-16:0-dihydrosphingomyelin (16:0-DHSM) show very similar packing properties, except that the expanded-to-condensed phase transition (crystallization) occurs at a lower surface pressure for 16:0-DHSM. The measured surface