Streptavidin, Pacific Blue™ conjugate

Citations & References (8)

Streptavidin, Pacific Blue™ conjugate

Our streptavidin, Pacific Blue™ conjugate is a bright, blue-fluorescent probe with excitation/emission ∼410/455 nm. Streptavidin is a nonglycosylated biotin-binding proteinRead more

Catalog Number	Quantity
S11222	1 mg

Catalog number S11222

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Our streptavidin, Pacific Blue™ conjugate is a bright, blue-fluorescent probe with excitation/emission ∼410/455 nm. Streptavidin is a nonglycosylated biotin-binding protein that is isolated from bacteria and has a near-neutral isoelectric point. It is widely used to detect biotinylated probes, because it reportedly exhibits less nonspecific binding than does the glycosylated, biotin-binding protein, avidin.

View a selection guide for all Alexa Fluor™ streptavidin and avidin conjugates.

For Research Use Only. Not for use in diagnostic procedures.

Specifications

ColorYellow

DescriptionPacific Blue™ Conjugate Streptavidin, 1mg, Yellow, Solid Form, 407nm Absorbency, Pacific Blue™ Label or Dye, 410/455nm Excitation/Emission, Used in Blot Analysis, Flow Cytometry, Histochemical Applications, Immunoassays, –5 to –30°C Storage Temperature

Detection MethodFluorescence

Excitation/Emission410/455 nm

Flow Cytometer Laser Lines405

For Use With (Application)Flow Cytometry, Immunoassays, Histochemical Applications, Blot Analysis

FormulationSolid

Label or DyePacific Blue

Packaging1 Each

Product TypeStreptavidin Conjugate

Purity or Quality GradeTLC

Quantity1 mg

Regulatory StatusFor research use only (RUO)

Sample Volume1 mg

Shelf Life6 Months

Shipping ConditionRoom Temperature

Storage Buffer10mg/mL PBS

ConjugatePacific Blue

FormSolid

Product LinePacific Blue

Unit SizeEach

Contents & Storage

Contains 1 vial of streptavidin Pacific Blue™ conjugate.

Store in freezer (-5 to -30°C) and protect from light.

Frequently asked questions (FAQs)

I am planning to use a fluorescent streptavidin labeled conjugate. What are the storage conditions and shelf life for the lyophilized powder and reconstituted solution?

In the lyophilized powder form, the fluorescent streptavidin labeled conjugate is stable for six months when stored at -20 degrees C, desiccated, and protected from light. The reconstituted solution is stable for approximately six months when stored at 4 degrees C, protected from light, with the addition of sodium azide to a final concentration of 5 mM or thimerosal to 0.2 mM. For longer storage, we recommend dividing the solution into aliquots and freezing at -20 degrees C, protected from light. Avoid repeated freezing and thawing of the solution.

I am planning to use a fluorescent streptavidin labeled conjugate. How should I prepare the working solution of the conjugate?

The fluorescent streptavidin labeled conjugate solution can be made by dissolving the powder in 0.5-1.0 mL of PBS or other suitable buffer. For details, please refer to page 4 of the "Streptavidin and Fluorescent Conjugates of Streptavidin" manual (https://assets.fishersci.com/TFS-Assets/LSG/manuals/mp00888.pdf).

Citations & References (8)

Citations & References

Abstract

Arenavirus nucleoprotein targets interferon regulatory factor-activating kinase IKKe.

Authors:Pythoud C, Rodrigo WW, Pasqual G, Rothenberger S, Martínez-Sobrido L, de la Torre JC, Kunz S,

Journal:J Virol

PubMed ID:22532683

'Arenaviruses perturb innate antiviral defense by blocking induction of type I interferon (IFN) production. Accordingly, the arenavirus nucleoprotein (NP) was shown to block activation and nuclear translocation of interferon regulatory factor 3 (IRF3) in response to virus infection. Here, we sought to identify cellular factors involved in innate antiviral signaling ... More

In vivo expansion of T reg cells with IL-2-mAb complexes: induction of resistance to EAE and long-term acceptance of islet allografts without immunosuppression.

Authors:Webster KE, Walters S, Kohler RE, Mrkvan T, Boyman O, Surh CD, Grey ST, Sprent J,

Journal:J Exp Med

PubMed ID:19332874

'Via a transcription factor, Foxp3, immunoregulatory CD4(+)CD25(+) T cells (T reg cells) play an important role in suppressing the function of other T cells. Adoptively transferring high numbers of T reg cells can reduce the intensity of the immune response, thereby providing an attractive prospect for inducing tolerance. Extending our ... More

Rapid lytic granule convergence to the MTOC in natural killer cells is dependent on dynein but not cytolytic commitment.

Authors:Mentlik AN, Sanborn KB, Holzbaur EL, Orange JS,

Journal:Mol Biol Cell

PubMed ID:20444980

'Natural killer cells are lymphocytes specialized to participate in host defense through their innate ability to mediate cytotoxicity by secreting the contents of preformed secretory lysosomes (lytic granules) directly onto a target cell. This form of directed secretion requires the formation of an immunological synapse and occurs stepwise with actin ... More

Immunosuppressive property within the Streptococcus pneumoniae cell wall that inhibits generation of T follicular helper, germinal center, and plasma cell response to a coimmunized heterologous protein.

Authors:Saumyaa, Arjunaraja S, Pujanauski L, Colino J, Torres RM, Snapper CM,

Journal:

PubMed ID:23817619

'We previously demonstrated that intact, inactivated Streptococcus pneumoniae (unencapsulated strain R36A) inhibits IgG responses to a number of coimmunized soluble antigens (Ags). In this study, we investigated the mechanism of this inhibition and whether other extracellular bacteria exhibited similar effects. No inhibition was observed if R36A was given 24 h ... More

Thymocytes may persist and differentiate without any input from bone marrow progenitors.

Authors:Peaudecerf L, Lemos S, Galgano A, Krenn G, Vasseur F, Di Santo JP, Ezine S, Rocha B,

Journal:J Exp Med

PubMed ID:22778388

'Thymus transplants can correct deficiencies of the thymus epithelium caused by the complete DiGeorge syndrome or FOXN1 mutations. However, thymus transplants were never used to correct T cell-intrinsic deficiencies because it is generally believed that thymocytes have short intrinsic lifespans. This notion is based on thymus transplantation experiments where it ... More

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