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Citations & References (16)
Invitrogen™
TetraSpeck™ Fluorescent Microspheres Size Kit (mounted on slide)
This TetraSpeck Fluorescent Microspheres Size Kit contains one microscope slide, with six viewing regions. Each of five viewing regions containsRead more
| Catalog Number | Quantity |
|---|---|
| T14792 | 1 Kit |
Catalog number T14792
Price (INR)Request A Quote
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Quantity:
1 Kit
This TetraSpeck Fluorescent Microspheres Size Kit contains one microscope slide, with six viewing regions. Each of five viewing regions contains microspheres of one size – 0.1, 0.2, 0.5, 1.0 or 4.0 μm. The remaining region contains a mixture of all five bead sizes. Every microsphere is stained with four different fluorescent dyes [365⁄430 nm (blue), 505⁄515 nm (green), 560⁄580 nm (orange), and 660⁄680 nm (dark red)] that have well-separated excitation and emission peaks. Invitrogen’s TetraSpeck™ fluorescent microspheres aid in the calibration of wide field, TIRF and confocal fluorescence microscopes,especially for multicolor applications like co-localization analysis and the derivation of point spread functions critical for deconvolution.
See our full collection of microscope calibration reagents ›
See our full collection of microscope calibration reagents ›
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Calibration TypeConfocal Microscope Calibration, Fluorescence Microscope Calibration
FormatMounted on Slide
Product LineTetraSpeck
Quantity1 Kit
Shipping ConditionRoom Temperature
ColorOrange, Dark Red, Blue, Green
Diameter (Metric)0.5, 0.2, 0.1, 1, 4μm
Product TypeFluorescent Microspheres Size Kit
Unit Size1 kit
Contents & Storage
Store at room temperature and protect from light.
Frequently asked questions (FAQs)
What are the excitation/emission peaks for TetraSpeck Microspheres?
The TetraSpeck Microspheres (Cat. Nos. T7279, T7280, T7281, T7283, T7284, T14792) are stained throughout with four different fluorescent dyes, yielding beads that each display four well-separated excitation/emission peaks at 360/430 nm (blue), 505/515 nm (green), 560/580 nm (orange) and 660/680 nm (dark red).
TetraSpeck Blue Dye Spectra
Fluorescence excitation and emission spectra of bead encapsulated TetraSpeck blue dye.
TetraSpeck Orange Dye Spectra
TetraSpeck Green Dye Spectra
TetraSpeck Dark Red Dye Spectra
Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.
Citations & References (16)
Citations & References
Abstract
The cohesion protein ORD is required for homologue bias during meiotic recombination.
Journal:J Cell Biol
PubMed ID:15007062
'During meiosis, sister chromatid cohesion is required for normal levels of homologous recombination, although how cohesion regulates exchange is not understood. Null mutations in orientation disruptor (ord) ablate arm and centromeric cohesion during Drosophila meiosis and severely reduce homologous crossovers in mutant oocytes. We show that ORD protein localizes along
Exocytosis of IgG as mediated by the receptor, FcRn: an analysis at the single-molecule level.
Journal:Proc Natl Acad Sci U S A
PubMed ID:15258288
'IgG transport within and across cells is essential for effective humoral immunity. Through a combination of biochemical and in vivo analyses, the MHC class I-related neonatal Fc receptor (FcRn) is known to play a central role in delivering IgGs within and across cells. However, little is known about the molecular
Colocalization of fluorescent markers in confocal microscope images of plant cells.
Journal:Nat Protoc
PubMed ID:18388944
'This protocol describes the steps needed to perform quantitative statistical colocalization on two-color confocal images, specifically of plant cells. The procedure includes a calibration test to check the chromatic alignment of the confocal microscope. A software tool is provided to calculate the Pearson and Spearman correlation coefficients (''Pearson-Spearman correlation colocalization''
Mast cell degranulation requires N-ethylmaleimide-sensitive factor-mediated SNARE disassembly.
Journal:J Immunol
PubMed ID:14607937
'Mast cells possess specialized granules that, upon stimulation of surface FcR with IgE, fuse with the plasma membrane, thereby releasing inflammatory mediators. A family of membrane fusion proteins called SNAREs, which are present on both the granule and the plasma membrane, plays a role in the fusion of these granules
Three-dimensional random access multiphoton microscopy for functional imaging of neuronal activity.
Journal:Nat Neurosci
PubMed ID:18432198
The dynamic ability of neuronal dendrites to shape and integrate synaptic responses is the hallmark of information processing in the brain. Effectively studying this phenomenon requires concurrent measurements at multiple sites on live neurons. Substantial progress has been made by optical imaging systems that combine confocal and multiphoton microscopy with