5(6)-TAMRA-X, SE (6-(Tetramethylrhodamine-5-(and-6)-Carboxamido) Hexanoic Acid, Succinimidyl Ester), mixed isomers
Citations & References (7)
Invitrogen™
5(6)-TAMRA-X, SE (6-(Tetramethylrhodamine-5-(and-6)-Carboxamido) Hexanoic Acid, Succinimidyl Ester), mixed isomers
The amine-reactive 6-(tetramethylrhodamine-5-(and-6)-carboxamido)hexanoic acid, succinimidyl ester (5(6)-TAMRA-X, SE) can be used to can be used to create bright red-orange fluorescentRead more
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Catalog Number
Quantity
T6105
also known as T-6105
10 mg
Catalog number T6105
also known as T-6105
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Quantity:
10 mg
The amine-reactive 6-(tetramethylrhodamine-5-(and-6)-carboxamido)hexanoic acid, succinimidyl ester (5(6)-TAMRA-X, SE) can be used to can be used to create bright red-orange fluorescent bioconjugates with excitation/emission maxima ∼555/580 nm.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Chemical ReactivityAmine
Emission580
Excitation555
Label or DyeTAMRA™ Isomers, TMR (Tetramethylrhodamine)
Product Type5(6)-TAMRA-X SE
Quantity10 mg
Reactive MoietyActive Ester, Succinimidyl Ester
Shipping ConditionRoom Temperature
Label TypeClassic Dyes
Unit Size10 mg
Contents & Storage
Store in freezer (-5 to -30°C) and protect from light.
Citations & References (7)
Citations & References
Abstract
FRET measurements of kinesin neck orientation reveal a structural basis for processivity and asymmetry.
'As the smallest and simplest motor enzymes, kinesins have served as the prototype for understanding the relationship between protein structure and mechanochemical function of enzymes in this class. Conventional kinesin (kinesin-1) is a motor enzyme that transports cargo toward the plus end of microtubules by a processive, asymmetric hand-over-hand mechanism. ... More
Spectral fluctuation of a single fluorophore conjugated to a protein molecule.
Authors:Wazawa T, Ishii Y, Funatsu T, Yanagida T
Journal:Biophys J
PubMed ID:10692340
'We have measured the fluorescence spectra of a single fluorophore attached to a single protein molecule in aqueous solution using a total internal reflection fluorescence microscope. The most reactive cysteine residue of myosin subfragment-1 (S1) was labeled with tetramethylrhodamine. The spectral shift induced by a change in solvent from aqueous ... More
Differential association of CD45 isoforms with CD4 and CD8 regulates the actions of specific pools of p56lck tyrosine kinase in T cell antigen receptor signal transduction.
Authors:Dornan S, Sebestyen Z, Gamble J, Nagy P, Bodnar A, Alldridge L, Doe S, Holmes N, Goff LK, Beverley P, Szollosi J, Alexander DR
Journal:J Biol Chem
PubMed ID:11694532
'An investigation into the role of CD45 isoforms in T cell antigen receptor signal transduction was carried out by transfecting CD45-negative CD4(+)CD8(+) HPB-ALL T cells with the CD45R0, CD45RBC, and CD45RABC isoforms. Fluorescence resonance energy transfer analysis showed that the CD45R0 isoform, but not the CD45RBC or CD45RABC isoforms, was ... More
A fluorescence polarization assay for screening inhibitors against the ribonuclease H activity of HIV-1 reverse transcriptase.
Authors:Nakayama GR, Bingham P, Tan D, Maegley KA
Journal:Anal Biochem
PubMed ID:16527235
A fluorescence polarization (FP) microplate assay suitable for screening compounds against the ribonuclease H (RNase H) activity of HIV-1 reverse transcriptase has been developed. This homogeneous assay uses a hybrid 18-mer DNA/RNA duplex substrate composed of an RNA oligonucleotide labeled with 6-carboxytetramethyl rhodamine at the 3' end that is annealed ... More
Single Molecule Detection Technologies in Miniaturized High Throughput Screening: Fluorescence Correlation Spectroscopy.
Authors:Moore KJ, Turconi S, Ashman S, Ruediger M, Haupts U, Emerick V, Pope AJ
Journal:J Biomol Screen
PubMed ID:10838431
Fluorescence assay technologies used for miniaturized high throughput screening are broadly divided into two classes. Macroscopic fluorescence techniques (encompassing conventional fluorescence intensity, anisotropy [also often referred to as fluorescence polarization] and energy transfer) monitor the assay volume- and time-averaged fluorescence output from the ensemble of emitting fluorophores. In contrast, single-molecule ... More