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Thermo Scientific™

GeneBLAzer™ CALCRL:RAMP2-CRE-bla FreeStyle™ 293F Cells

Catalog number:  K1438

Related applications:

Pharma & Biopharma

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Description

GeneBLAzer™ CALCRL:RAMP2-CRE-bla FreeStyle™ 293F cells contain the human Adrenomedullin Receptor 1 (CALCRL:RAMP2), (Accession #’s NM_005795.3, BC02795.1) stably integrated into the CellSensor™ CRE-bla Freestyle™ 293F cell line. CellSensor™ CRE-bla Freestyle™ 293F cells (Cat. no. K1636) contain a beta-lactamase (bla) reporter gene under control of the cAMP response element (CRE).

GeneBLAzer™ CALCRL:RAMP2-CRE-bla FreeStyle™ 293F cells are functionally validated for Z’-factor and EC50 concentrations of Adrenomedullin(1-52). In addition, GeneBLAzer™ CALCRL:RAMP2-CRE-bla FreeStyle™ 293F cells have been tested for assay performance under variable conditions. These data are found in the Validation & Assay Performance Summary.

For Research Use Only. Not for use in diagnostic procedures.

Specifications

Accession Number: NM_005855.2
Assay Entry: Cell-based beta lactamase reporter gene
Cell Line: FreeStyle™ 293-F Cells
Cell State: Dividing Cells
Detection Method: Fluorescent
Druggable Target: GPCRs
Gene Symbol: CALCRL:RAMP2
Human Protein Class: G-Protein Coupled Receptor (GPCR)
Population Status: Single Cell Clone Origin
Product Line: GeneBLAzer™
Readout: End Point
System Type: GeneBLAzer™
Target Entry: CALCRL:RAMP2
Technique: FRET

Contents & storage

Contents:
K1438 GeneBLAzer™ CALCRL:RAMP2-CRE-bla FreeStyle™ 293 F cells
(LiveBLAzer™-FRET B/G Loading Kit, Solution D and other materials are required separately; please refer to the protocol).
Includes:
• GeneBLAzer™ CALCRL:RAMP2-CRE-bla FreeStyle™ 293 F cells
• GeneBLAzer™ CALCRL:RAMP2-CRE-bla FreeStyle™ 293 F Assay Protocol
• Certificate of Analysis

Storage:
The GeneBLAzer™ CALCRL:RAMP2-CRE-bla FreeStyle™ 293 F cells are shipped on dry ice and should be stored in liquid nitrogen immediately upon receipt.