Ham's F-12 Nutrient Mix
Ham's F-12 Nutrient Mix
Gibco™

Ham's F-12 Nutrient Mix

Ham's F-12 Nutrient Mixture (F-12) was designed for serum-free, single-cell plating of Chinese Hamster Ovary (CHO) cells. F-12 has sinceRead more
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Catalog NumberQuantity
11765054500 mL
1176506210 x 500 mL
117650471000 mL
117650706 x 1000 mL
Catalog number 11765054
Price (KRW)/ 500 mL
Price:56,000
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Quantity:
500 mL
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Price (KRW)/ 500 mL
Price:56,000
Add to cart
Ham's F-12 Nutrient Mix
Catalog number11765054
Price (KRW)/ 500 mL
Price:56,000
-
Add to cart

Ham's F-12 Nutrient Mixture (F-12) was designed for serum-free, single-cell plating of Chinese Hamster Ovary (CHO) cells. F-12 has since been used for serum-free growth of CHO cultures as well as serum-supplemented growth of other mammalian cells, including chondrocytes and rat prostate epithelial cells. We offer a variety of F-12 modifications for a range of cell culture applications. Find the right formulation using the media selector tool.


This Ham's F-12 is modified as follows:
With
• L-glutamine
• Phenol Red


The complete formulation is available.

Using F-12
Compared to other basal media, F-12 contains a wider variety of components, including zinc, putrescine, hypoxanthine, and thymidine. F-12 contains no proteins or growth factors. Therefore, F-12 requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). F-12 uses a sodium bicarbonate buffer system (1.176 g/L), and therefore requires a 5–10% CO2 environment to maintain physiological pH.

Specifications
Cell LineCHO, COS-7, and rat prostate epithelial cells
Cell TypePrimary Rat Astrocytes
ClassificationAnimal Origin-free
Concentration1 X
FormLiquid
Product TypeHam's F-12 Nutrient Mixture
Shelf Life12 Months From Date of Manufacture
SterilitySterile-filtered
With AdditivesGlutamine, Phenol Red, Sodium Pyruvate
Without AdditivesNo HEPES
Green FeaturesSustainable packaging
Manufacturing QualitycGMP-compliant under the ISO 13485 standard
Product LineGibco™
Quantity500 mL
Shipping ConditionRoom Temperature
Unit Size500 mL
Contents & Storage
Storage conditions: 2°C to 8°C. Protect from light
Shipping conditions: Ambient
Shelf life: 12 months from date of manufacture