HBSS, no calcium, no magnesium, no phenol red
HBSS, no calcium, no magnesium, no phenol red
Actual product may vary
Citations & References (3)
Gibco™

HBSS, no calcium, no magnesium, no phenol red

Hanks' Balanced Salt Solution (HBSS) is used for a variety of cell culture applications, such as washing cells before dissociation,Read more
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Catalog NumberQuantity
1417510310 x 500 mL
14175095500 mL
141750791000 mL
141751456 x 1000 mL
Catalog number 14175103
Price (KRW)
325,000
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Ends: 31-Dec-2025
342,000
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Quantity:
10 x 500 mL
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Price (KRW)
325,000
Online offer
Ends: 31-Dec-2025
342,000
Save 17,000 (5%)
Each
Add to cart

Hanks' Balanced Salt Solution (HBSS) is used for a variety of cell culture applications, such as washing cells before dissociation, transporting cells or tissue samples, diluting cells for counting, and preparing reagents. Formulations without calcium and magnesium are required for rinsing chelators from the culture before cell dissociation.

We offer a variety of Gibco™ HBSS formulations for a range of cell culture applications.


This HBSS is modified as follows:
WithWithout
• Glucose• Calcium
 • Magnesium
 • Phenol Red


The complete formulation is available.

For Research Use or Further Manufacturing. Not for diagnostic use or direct administration into humans or animals.
Specifications
Chemical Name or MaterialHanks' Balanced Salt Solution (HBSS)
ColorClear
IncludesNo Sodium Pyruvate
Manufacturing QualitycGMP-compliant under the ISO 13485 standard
Osmolality270 - 295 mOsm/kg
Recommended StorageStorage conditions: 15°C to 30°C
Shipping conditions: Room temperature
Shelf life: 36 months from date of manufacture
SterilitySterile-filtered
Concentration1 X
For Use With (Application)Mammalian Cell Culture
Physical FormLiquid
Product LineGibco
Quantity10 x 500 mL
Solution TypeHank's Balanced Salt
pH6.7 - 7.8
Unit SizeEach

Frequently asked questions (FAQs)

Can I store Hank's Balanced Salt Solution (HBSS) in the refrigerator?

The recommended storage condition for our HBSS products is at 15-30 degrees C. Generally, storage at 4 degrees C will not impact these products. The only concern would be for the 10x HBSS solutions with calcium and magnesium as these salts might precipitate when stored outside the recommended temperature ranges. As long as you don't see any precipitation upon refrigeration, the product will be fine.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Do you offer media without phenol red (phenol red-free media)?

Yes, please visit this page for a list of our phenol red-free media (http://tools.thermofisher.com/content/sfs/brochures/Phenol_RedFree_Media_Glance.pdf).

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Citations & References (3)

Citations & References
Abstract
Role of hepatic transporters in the disposition and hepatotoxicity of a HER2 tyrosine kinase inhibitor CP-724,714.
Authors:Feng B, Xu JJ, Bi YA, Mireles R, Davidson R, Duignan DB, Campbell S, Kostrubsky VE, Dunn MC, Smith AR, Wang HF,
Journal:Toxicol Sci
PubMed ID:19223659
'CP-724,714, a potent and selective orally active HER2 tyrosine kinase inhibitor, was discontinued from clinical development due to unexpected hepatotoxicity in cancer patients. Based on the clinical manifestation of the toxicity, CP-724,714 likely exerted its hepatotoxicity via both hepatocellular injury and hepatobiliary cholestatic mechanisms. The direct cytotoxic effect, hepatobiliary disposition ... More
Isolation of carbohydrate-specific CD4(+) T cell clones from mice after stimulation by two model glycoconjugate vaccines.
Authors:Avci FY, Li X, Tsuji M, Kasper DL,
Journal:Nat Protoc
PubMed ID:23196974
Here we describe how to isolate carbohydrate-specific T cell clones (for which we propose the designation 'Tcarbs') after stimulation by two glycoconjugate vaccines. We describe how to prepare, purify and characterize two model glycoconjugate vaccines that can be used to generate Tcarbs. These glycoconjugate vaccines (GBSIII-OVA and GBSIII-TT) are synthesized ... More
Ceramide mediates age-associated increase in macrophage cyclooxygenase-2 expression.
Authors: Claycombe Kate J; Wu Dayong; Nikolova-Karakashian Mariana; Palmer Helen; Beharka Alison; Paulson K Eric; Meydani Simin Nikbin;
Journal:J Biol Chem
PubMed ID:12072440
Previously, we showed that macrophages (MØ) from old mice have significantly higher levels of lipopolysaccharide (LPS)-induced prostaglandin E(2) (PGE(2)) production than young mice, due to increased cyclooxygenase-2 (COX-2) mRNA levels. The aim of the current study was to determine the underlying mechanisms of age-associated increase in COX-2 gene expression. The ... More