SuperScript™ II Reverse Transcriptase
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SuperScript™ II Reverse Transcriptase
Invitrogen™

SuperScript™ II Reverse Transcriptase

Invitrogen SuperScript II Reverse Transcriptase is a genetically engineered MMLV reverse transcriptase (RT) with reduced RNase H activity and increasedRead more
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Catalog NumberNo. of Reactions
1806401450 Reactions
1806402210 Reactions
18064071200 Reactions
Catalog number 18064014
Price (KRW)
485,000
Online offer
Ends: 31-Dec-2025
538,000
Save 53,000 (10%)
Each
Add to cart
No. of Reactions:
50 Reactions
Request bulk or custom format
Price (KRW)
485,000
Online offer
Ends: 31-Dec-2025
538,000
Save 53,000 (10%)
Each
Add to cart

Invitrogen SuperScript II Reverse Transcriptase is a genetically engineered MMLV reverse transcriptase (RT) with reduced RNase H activity and increased thermal stability compared to wild-type MMLV RT. Mutations in the RNase H domain of the enzyme eliminate degradation of the RNA during first-strand cDNA synthesis, which results in higher yields of full-length cDNA.

SuperScript reverse transcriptases are the most highly trusted and widely used RTs with over 50,000 citations, reviews, and publications to date. The enzyme is purified to near homogeneity from E. coli containing the modified pol gene of Moloney Murine Leukemia Virus.

Features of SuperScript II Reverse Transcriptase include:
• High cDNA yields and more full-length product
• Generation of cDNA up to 12.3 kb
• First-strand cDNA synthesis at higher temperatures than conventional MMLV RT

Note: The latest member of the SuperScript RT family, SuperScript IV Reverse Transcriptase, features enhanced thermostability, processivity, yields, and performance with any RNA samples, including those of suboptimal purity or integrity.

For Research Use Only. Not for use in diagnostic procedures.
Specifications
Final Product TypeFirst-Strand cDNA
FormatStand-alone Enzyme
No. of Reactions50 Reactions
Optimal Reaction Temperature42°C
Quantity10,000 Units
Reaction FormatSeparate components
Reagent TypeReverse Transcription
Reverse TranscriptaseSuperScript II
Ribonuclease H ActivityReduced
Shipping ConditionWet Ice
Size (Final Product)Up to 12.3 kb
Starting MaterialRNA
TechniqueReverse Transcription
Concentration200 U/μL
GC-Rich PCR PerformanceHigh
Reaction Speed50 min.
Unit SizeEach
Contents & Storage

• SuperScript II Reverse Transcriptase, 50 μL (10,000 units at 200 U/μL)
• 5X First-strand buffer
• 100 mM DTT

Store at –20°C.

Frequently asked questions (FAQs)

How can I remove genomic DNA contamination from my sample prior to performing RT-PCR?

We recommend using ezDNase (Cat. No. 11766051). ezDNase Enzyme's high specificity for double-stranded DNA enables efficient and fast genomic DNA removal without reduction in the quality or quantity of RNA. ezDNase Enzyme is heat-labile and so can be easily deactivated by heat treatment at moderate temperature (55 degrees C). These features make ezDNase Enzyme an excellent choice for genomic DNA removal prior to reverse transcription reactions.

How much RNA should be employed for first-strand cDNA synthesis?

The amount of RNA template for a cDNA synthesis is highly flexible and depends upon the amount of sample available and an individual's need. In general, 1 µg total RNA is used in a typical 20-µL RT reaction.

Find additional tips, troubleshooting help, and resources within ourReverse Transcription and RACE Support Center.

Should I treat the cDNA with RNase H prior to downstream processing?

RNase H treatment is not always necessary. Many PCR reactions work without it. However, for cDNA synthesized with RNase H-deficient reverse transcriptases (like SuperScript II, III, and IV), RNA/cDNA hybrids—especially GC-rich ones—may not denature well, reducing PCR sensitivity. RNase H treatment can help in such cases. Additionally, RNase H treatment is beneficial for cloning larger fragments.

What percentage of RNA is converted to cDNA when performing reverse transcription?

This depends highly on the quality of the sample. mRNA itself makes up 1-5% of total RNA. Depending on the primer and enzyme used, reverse transcription can covert >70% of that into cDNA.

Find additional tips, troubleshooting help, and resources within our Reverse Transcription and RACE Support Center.

I'm setting up my RT reaction and am trying to decide whether I should use random primers, oligo(dT) primer, gene-specific primer, or oligo(dT)/random mix primers. What would you suggest?

Random primers are the best choice for degraded RNA, RNA with heavy secondary structure, non-polyadenylated RNA, or prokaryotic RNA. It is recommended only for two-step RT-PCR, and typically gives the highest yields, although the cDNA may not necessarily be full length. Oligo(dT) primers are good to use when trying to recover full-length cDNA from 2-step RT-PCR. The reaction is influenced by secondary structure and RNA quality. Gene specific primers should be used for very specific, mainly one-step RT-PCR reactions.

Find additional tips, troubleshooting help, and resources within our Reverse Transcription and RACE Support Center.

View all SuperScript™ II Reverse Transcriptase, 10,000 Units FAQs