DMEM, high glucose, no glutamine, no methionine, no cystine
DMEM, high glucose, no glutamine, no methionine, no cystine
Citations & References (3)
Gibco™

DMEM, high glucose, no glutamine, no methionine, no cystine

Dulbecco's Modified Eagle Medium (DMEM) is a widely used basal medium for supporting the growth of many different mammalian cells.Read more
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Catalog NumberQuantity
21013024500 mL
Catalog number 21013024
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Ends: 31-Dec-2025
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500 mL
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Price (KRW)
79,000
Online offer
Ends: 31-Dec-2025
83,000
Save 4,000 (5%)
Each
Add to cart

Dulbecco's Modified Eagle Medium (DMEM) is a widely used basal medium for supporting the growth of many different mammalian cells. Cells successfully cultured in DMEM include primary fibroblasts, neurons, glial cells, HUVECs, and smooth muscle cells, as well as cell lines such as HeLa, 293, Cos-7, and PC-12. We offer a variety of Gibco™ DMEM modifications for a range of cell culture applications. Find the right formulation using the media selector tool.


This DMEM is modified as follows:
WithWithout
• High Glucose• L-glutamine
• Phenol Red• Sodium Pyruvate
 • HEPES
 • L-methionine
 • L-cystine


The complete formulation is available.

DMEM is unique from other media as it contains 4 times the concentration of amino acids and vitamins than the original Eagle's Minimal Essential Medium. DMEM was originally formulated with low glucose (1 g/L) and sodium pyruvate, but is often used with higher glucose levels, with or without sodium pyruvate.

Product Use
For Research Use Only: Not intended for animal or human diagnostic or therapeutic use.

DMEM contains no proteins, lipids, or growth factors. Therefore, DMEM requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). DMEM uses a sodium bicarbonate buffer system (3.7 g/L) and therefore requires a 5-10% CO2 environment to maintain physiological pH.

In vitro diagnostic. For Research Use Only: Not intended for animal or human diagnostic or therapeutic use.
Specifications
Amino AcidsNo Cystine, No Methionine
Cell LineHeLa, 293, Cos-7, and PC-12
Cell TypePrimary Fibroblasts, Neurons, Glial Cells, HUVECs, Smooth Muscle Cells
Concentration1 X
Manufacturing QualitycGMP-compliant under the ISO 13485 standard
Product LineGibco
Product TypeDMEM (Dulbecco's Modified Eagle Medium)
Quantity500 mL
Shelf Life12 Months From Date of Manufacture
Shipping ConditionRoom Temperature
ClassificationAnimal Origin-free
FormLiquid
Serum LevelStandard Serum Supplementation
SterilitySterile-filtered
With AdditivesHigh Glucose, Phenol Red
Without AdditivesNo Glutamine, No HEPES, No Sodium Pyruvate, No Methionine, No Cystine
Unit SizeEach
Contents & Storage
Storage conditions: 2-8° C. Protect from light
Shipping conditions: Ambient
Shelf life: 12 months from date of manufacture

Frequently asked questions (FAQs)

What is the manganese concentration in DMEM? Do you offer manganese-free DMEM?

Manganese is not present in the formulation of our catalog DMEM media products.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

How long can I keep my media after supplementing with serum?

Generally speaking, media can be used for up to three weeks after supplementation with serum. There are no formal studies to support this, but it is the rule of thumb used by our scientists.

Find additional tips, troubleshooting help, and resources within our Mammalian Cell Culture Basics Support Center.

My medium was shipped at room temperature but it is supposed to be stored refrigerated. Is it okay?

We routinely ship media that require long-term storage in the refrigerator at room temperature. We have done studies on representative media formulations to show that media can be at room temperature for up to a week without a problem.

Find additional tips, troubleshooting help, and resources within our Mammalian Cell Culture Basics Support Center.

How can I remove mycoplasma contamination from my cell culture medium?

Very often mycoplasma contamination cannot be removed from the culture so it should be discarded. You may have a unique culture that you prefer not to discard and would like to try to clean it. Ciprofloxacin and Plasmocin have reportedly been used for this application. If interested in a protocol or directions for use, check with the antibiotic supplier or published literature. Note that mycoplasma are very difficult to remove from culture and spread easily so the treated cultures should be quarantined until clear of mycoplasma, and your laboratory should be thoroughly cleaned.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

I see a decrease in growth of my culture. What should I do?

Try changing the medium or serum. Compare media formulations for differences in glucose, amino acids, and other components. Compare an old lot of serum with a new lot. Increase initial cell inoculums. Lastly, adapt cells sequentially to new medium.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

View all DMEM, high glucose, no glutamine, no methionine, no cystine FAQs

Citations & References (3)

Citations & References
Abstract
Enhanced expression of the human vacuolar H+-ATPase c subunit gene (ATP6L) in response to anticancer agents.
Authors: Torigoe Takayuki; Izumi Hiroto; Ishiguchi Hiroshi; Uramoto Hidetaka; Murakami Tadashi; Ise Tomoko; Yoshida Yoichiro; Tanabe Mizuho; Nomoto Minoru; Itoh Hideaki; Kohno Kimitoshi;
Journal:J Biol Chem
PubMed ID:12133827
'We have isolated two overlapping genomic clones that contain the 5''-terminal portion of the human vacuolar H(+)-ATPase c subunit (ATP6L) gene. The sequence preceding the transcription initiation site, which is GC-rich, contains four GC boxes and one Oct1-binding site, but there is no TATA box or CCAAT box. In vivo ... More
Functional linkage between the endoplasmic reticulum protein Hsp47 and procollagen expression in human vascular smooth muscle cells.
Authors:Rocnik EF, van der Veer E, Cao H, Hegele RA, Pickering JG
Journal:J Biol Chem
PubMed ID:12163502
'Hsp47 is a heat stress protein that interacts with procollagen in the lumen of the endoplasmic reticulum, which is vital for collagen elaboration and embryonic viability. The precise actions of Hsp47 remain unclear, however. To evaluate the effects of Hsp47 on collagen production we infected human vascular smooth muscle cells ... More
CCAAT/Enhancer Binding Protein alpha Binds to the Epstein-Barr Virus (EBV) ZTA Protein through Oligomeric Interactions and Contributes to Cooperative Transcriptional Activation of the ZTA Promoter through Direct Binding to the ZII and ZIIIB Motifs during Induction of the EBV Lytic Cycle.
Authors:Wu FY, Wang SE, Chen H, Wang L, Hayward SD, Hayward GS,
Journal:J Virol
PubMed ID:15078966
'The Epstein-Barr virus (EBV)-encoded ZTA protein interacts strongly with and stabilizes the cellular CCAAT/enhancer binding protein alpha (C/EBPalpha), leading to the induction of p21-mediated G(1) cell cycle arrest. Despite the strong interaction between these two basic leucine zipper (bZIP) family proteins, the ZTA and C/EBPalpha subunits do not heterodimerize, as ... More