Dulbecco's Modified Eagle's Limiting Medium (DMEM-LM)
Dulbecco's Modified Eagle's Limiting Medium (DMEM-LM)
Dulbecco's Modified Eagle's Limiting Medium (DMEM-LM)
Dulbecco's Modified Eagle's Limiting Medium (DMEM-LM)
Citations & References (3)
Thermo Scientific™

Dulbecco's Modified Eagle's Limiting Medium (DMEM-LM)

Formulation: Sterile filtered DMEM (-)L-leucine, (-)L-methionine with 4.5g/L glucose, 4.0mM L-glutamine, sodium pyruvate and phenol red.Related ProductsL-Photo-LeucineL-Photo-MethionineRead more
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Catalog NumberQuantity
30030500 mL
Catalog number 30030
Price (KRW)
88,000
Online offer
Ends: 31-Dec-2025
103,000
Save 15,000 (15%)
Each
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Quantity:
500 mL
Request bulk or custom format
Price (KRW)
88,000
Online offer
Ends: 31-Dec-2025
103,000
Save 15,000 (15%)
Each
Add to cart
Formulation: Sterile filtered DMEM (-)L-leucine, (-)L-methionine with 4.5g/L glucose, 4.0mM L-glutamine, sodium pyruvate and phenol red.

Related Products
L-Photo-Leucine
L-Photo-Methionine
For Research Use Only. Not for use in diagnostic procedures.
Specifications
DescriptionPierce Dulbecco's Modified Eagle's Limiting Medium (DMEM-LM)
Product LinePierce
Product TypeCrosslinker
Quantity500 mL
FormLiquid
Unit SizeEach
Contents & Storage
Upon receipt store at 4°C.

Frequently asked questions (FAQs)

Apart from proteins, will the Photoreactive Amino Acids crosslink to other molecules?

Photoreactive amino acids are incorporated into proteins during synthesis; but upon UV activation, they can crosslink to other biomolecules within proximity.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

How stable are the Photoreactive Amino Acids in DMEM-LM?

Long-term stability of the photoreactive amino acids in media has not been determined but should be comparable to their natural analogs, if protected from light. For best results, store the photoreactive amino acids at -20°C as a dry compound. Just before use, add the photoreactive amino acids to the minimal volume of DMEM-LM supplemented with dialyzed serum.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

How long do I need to incubate the Photoreactive Amino Acids with my cells?

Incubate the photoreactive amino acids in DMEM-LM with cells for 24 hours. For proteins with high turnover, a minimum incubation of 8 hours is needed for detectable crosslinking levels. Incubation for longer than 24 hours might significantly affect cell growth or viability.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

My cells will not grow in DMEM, what other type of culture media can be used with the Photoreactive Amino Acids?

Some cells types can be adapted to grow in DMEM before using the DMEM-LM supplemented with the photoreactive amino acids. Currently we do not offer any other leucine- and methionine-depleted culture medium.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

Can I use the Photoreactive Amino Acids to crosslink peptides?

Peptides can be synthesized using the N-termini-protected (Boc or Fmoc) photoreactive amino acid derivatives.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

View all Dulbecco's Modified Eagle's Limiting Medium (DMEM-LM) FAQs

Citations & References (3)

Citations & References
Abstract
Mass spectrometry of laser-initiated carbene reactions for protein topographic analysis.
Authors:Jumper CC, Schriemer DC
Journal:Anal Chem
PubMed ID:21425771
'We report a protein labeling method using nonselective carbene reactions of sufficiently high efficiency to permit detection by mass spectrometric methods. The approach uses a diazirine-modified amino acid (l-2-amino-4,4''-azipentanoic acid, "photoleucine") as a label source, which is converted to a highly reactive carbene by pulsed laser photolysis at 355 nm. ... More
The copper binding domain of SPARC mediates cell survival in vitro via interaction with integrin beta1 and activation of integrin-linked kinase.
Authors:Weaver MS, Workman G, Sage EH
Journal:J Biol Chem
PubMed ID:18503049
'Secreted protein acidic and rich in cysteine (SPARC) is important for the normal growth and maintenance of the murine lens. SPARC-null animals develop cataracts associated with a derangement of the lens capsule basement membrane and alterations in lens fiber morphology. Cellular stress and disregulation of apoptotic pathways within lens epithelial ... More
Inhibition of calcineurin-mediated endocytosis and alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA) receptors prevents amyloid beta oligomer-induced synaptic disruption.
Authors:Zhao WQ, Santini F, Breese R, Ross D, Zhang XD, Stone DJ, Ferrer M, Townsend M, Wolfe AL, Seager MA, Kinney GG, Shughrue PJ, Ray WJ
Journal:J Biol Chem
PubMed ID:20032460
Synaptic degeneration, including impairment of synaptic plasticity and loss of synapses, is an important feature of Alzheimer disease pathogenesis. Increasing evidence suggests that these degenerative synaptic changes are associated with an accumulation of soluble oligomeric assemblies of amyloid beta (Abeta) known as ADDLs. In primary hippocampal cultures ADDLs bind to ... More