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SuperSignal™ West Femto Maximum Sensitivity Substrate
Thermo Scientific™

SuperSignal™ West Femto Maximum Sensitivity Substrate

Thermo Scientific SuperSignal West Femto Maximum Sensitivity Substrate is an ultra-sensitive enhanced chemiluminescent (ECL) substrate for low femtogram protein levelRead more
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Catalog NumberQuantity
34095X44 x 100 mL
3409420 mL
34095100 mL
34096X44 x 200 mL
34096200 mL
Catalog number 34095X4
Price (KRW)
2,390,000
Each
Quantity:
4 x 100 mL
Price (KRW)
2,390,000
Each

Thermo Scientific SuperSignal West Femto Maximum Sensitivity Substrate is an ultra-sensitive enhanced chemiluminescent (ECL) substrate for low femtogram protein level detection by western blot analysis with horseradish peroxidase (HRP) enzyme.

SuperSignal West Femto Maximum Sensitivity Substrate characteristics include:

  • Sensitivity: low-femtogram
  • Stability: 8-hr working solution stability, 1-year kit stability at 4°C
  • Compatibility: nitrocellulose and PVDF membranes
  • Signal duration: up to 8 hours
  • Recommended primary antibody concentration: 1:5,000–1:100,000 dilution (10–200 ng/mL)
  • Recommended secondary antibody concentration: 1:100,000–1:500,000 dilution (2–10 ng/mL)

SuperSignal West Femto Maximum Sensitivity Substrate is designed for low femtogram-level detection by western blot analysis. When combined with optimized antibody concentrations and blocking buffers, SuperSignal West Femto substrate enables detection of target proteins in amounts that are too small to be seen with typical ECL substrates.

For Research Use Only. Not for use in diagnostic procedures.
Specifications
Kit ContentsLuminol/Enhancer, 4 x 50 mL, Stable Peroxide Buffer, 4 x 50 mL
Recommended Antibody Concentrations2°: 1:100-500 K (2-10 ng/mL), 1°: 1:5 K (10- 200 ng/mL)
SensitivityLow to mid-femtogram
Signal Duration6 to 8 hr.
Detection MethodChemiluminescence
FormLiquid
Product LineSuperSignal
Product TypeSubstrate
Quantity4 x 100 mL
SubstrateHorseradish Peroxidase
Unit SizeEach
Contents & Storage
Sufficient For: 48 mini-gel blots; 4000 cm2 of membrane
• Luminol/Enhancer, 4 x 50 mL
• Stable Peroxide Buffer, 4 x 50 mL

Store reagents at 4°C.