QuantStudio™ 1 Real-Time PCR System, 96-well, 0.2 mL, laptop
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Applied Biosystems™

QuantStudio™ 1 Real-Time PCR System, 96-well, 0.2 mL, laptop

The Applied Biosystems QuantStudio 1 Real-Time PCR System makes quality and reliability the practical choice. This affordable instrument offers basicRead more
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Catalog NumberQuantity
A404271 System
Catalog number A40427
Price (KRW)
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1 System

The Applied Biosystems QuantStudio 1 Real-Time PCR System makes quality and reliability the practical choice. This affordable instrument offers basic features ideal for those new to qPCR or those who have a limited budget. Despite its affordability, the QuantStudio 1 system offers the quality, reliability, and advanced user experience of our QuantStudio family of instruments.

Features of the QuantStudio 1 system include:

  • Touchscreen—a large 8.5-inch interactive touchscreen
  • Fixed block—one fixed 96-well, 0.2-mL isothermal block with an easy-to-open manual drawer
  • Multiplexing capability—offers the three most commonly used excitation and emission filters: FAM, VIC, and ROX
  • Protocols—pre-optimized protocol templates
  • Software—intuitive software that supports analysis via web browser or desktop computer
  • Dyes—three filters calibrated for FAM/SYBR Green/SYTO 9, VIC/HEX/TET/JOE, and JUN/ROX/Texas Red dyes
  • Sensitivity—detect differences as small as 1.5-fold quantities in singleplex reactions

Thermo Fisher Connect

Thermo Fisher Connect with cloud-enabled services provides scientists with a secure place to store, analyze, and share data. Upload data from the instrument or upload your own files from anywhere you have web access. Applied Biosystems qPCR Analysis Modules within Thermo Fisher Connect are web browser-based software tools that are flexible, fast, and easy to use.

Easy to use

The QuantStudio 1 Real-Time PCR System is compact in form, factory calibrated, and delivered in user-friendly packaging to enable fast and simple installation for immediate use. The interactive touchscreen provides a modern usability experience, and the simplified instrument software/pre-optimized protocol templates allow users of any experience level to quickly set up and start a run.

Design and analysis software

Option 1: QuantStudio Design and Analysis cloud software

  • Supports web browser-based system configuration with PC or MAC computer
  • Streamlined software for improved usability and response time
  • Enables secure access of your data when and where you want through Thermo Fisher Connect
  • No software to install, no licenses and no versions to update

Option 2: QuantStudio Design and Analysis desktop software

  • Supports traditional co-located computer system configuration on Windows™ 7 and Windows™ 10 OS
  • Streamlined software for improved usability and response time
  • No licenses to purchase

Results you can trust

Improved well-to-well and instrument-to-instrument data accuracy is achieved with OptiFlex technology, which features three coupled excitation and emission filter channels with white LED.

Sensitive data analysis

Detect changes in target quantity as small as 1.5-fold in single-plex reactions and obtain 10 logs of linear dynamic range.

High application versatility

Simple and optimized protocols, reliable assays and reagents, and intuitive software are available for a broad range of applications, including gene expression, genetic variation, gene regulation, and more.

For Research Use Only. Not for use in diagnostic procedures.
Specifications
Depth (Metric)50 cm
Display Type8.5 in. Touchscreen
For Use With (Equipment)QuantStudio™
Format96-well Plate
Height (Metric)40 cm
Product LineApplied Biosystems™ QuantStudio
Quantity1 System
Reaction Volume Range10 to 100 μL
Shipping ConditionApproved for Shipment at Room Temperature or on Wet Ice
Weight<26 kg
Width (Metric)27 cm
Block Configurations96-well
Block FormatNon-interchangeable
Unit SizeEach
Contents & Storage
Laptop

Frequently asked questions (FAQs)

What is the difference between continuous melt curves and step and hold melt curves on the Applied Biosystems Real-Time PCR Instruments?

Continuous melt curve: increases the temperature by the ramp increment (degrees C/sec) so that the temperature will change at a constant rate set in the protocol.
Step and Hold melt curve: increases the temperature by the ramp increment (degrees C) and then holds at that temperature for the time specified by the user.

The amount of data collected will depend on the filter(s) selected. The more filters you use, the longer the acquisition will take and the fewer data points will be collected.

Find additional tips, troubleshooting help, and resources within our Real-Time PCR and Digital PCR Instruments Support Center.

Can I import a standard curve for my real-time PCR data into the Design & Analysis Software (v2.x)?

It is possible to import a standard curve into the Design & Analysis Software (v2.x) if you are analyzing results from one of the following Applied Biosystems real-time PCR instruments:
• 7900HT Fast Real-Time PCR System
• 7500/7500 Fast Real-Time PCR System
• StepOne/StepOne Plus Real-Time PCR System
• ViiA 7 Real Time PCR System
• QuantStudio 1 Real-Time PCR System
• QuantStudio 3 & 5 Real-Time PCR System
• QuantStudio 6 & 7 Flex Real-Time PCR System
• QuantStudio 6 & 7 Pro Real-Time PCR Systems
• QuantStudio 12K Flex Real-Time PCR system

Follow the instructions below to import a standard curve for analysis in the Design & Analysis Software (v2.x):
1. Open the data file in the Design & Analysis Software (v2.x).
2. Click on Actions.
3. Click on "Standard Curve Analysis Setting".
4. Select "External Standard Curves" and click on "Import".
5. Browse to the proper .csv file and click "Open".
6. Click "Apply".

Can I import a standard curve for my real-time PCR data into the QuantStudio Design & Analysis Software (v1.x)?

If you are analyzing results from the QuantStudio 1 Real-Time PCR System, QuantStudio 3 Real-Time PCR System, or QuantStudio 5 Real-Time PCR System, it is possible to import a standard curve into the QuantStudio Design & Analysis Software (v1.x) following the instructions below:
1. Open the data file in the QuantStudio Design & Analysis Software (v1.x).
2. Go to Analysis Settings.
3. Click on the "Standard Curve Settings" tab.
4. Select the option to "Use a standard curve imported from another experiment" and click on "Import".
5. Browse to and highlight the proper data file and click "Select".
6. Click Apply.

With the QuantStudio 1 instrument, why am I only able to export my run files to USB, whereas the cloud and network options are not available?

It is possible that the instrument is not connected to the computer and is not linked with the Thermo Fisher Cloud. Please see page 38 of the QuantStudio 1 Real-Time PCR System User Guide (https://assets.thermofisher.com/TFS-Assets/LSG/manuals/MAN0017853_QS1%20UG.pdf) for instructions to link an instrument profile with the Thermo Fisher Cloud. Although default installation instructions recommend using an Ethernet cable, it can also be done wirelessly using a High Power USB WiFi Module, Cat. No. A26774 (https://www.thermofisher.com/order/catalog/product/A26774).

If you experience connection issues between the instrument and computer, it might be useful setting a static IP address on both the instrument and computer. On the computer, you can use online help, such as this one (https://kb.netgear.com/27476/How-do-I-set-a-static-IP-address-in-Windows) to set the static IP address. Here are the details for the settings:

- Computer IP: 10.0.0.2
- Instrument IP: 10.0.0.3
- Subnet: 255.255.255.0
- DNS1: 8.8.8.8
- DNS2: 8.8.4.4
- Default gateway: 10.0.0.1

On the instrument, the IP address can be found under Settings and About Instrument. If you see an IP address but are still having network issues, try to PING the IP Address in a command window of the computer:

- Select the windows start button and select the RUN command.
- Open the Run command and type in CMD.
- This will open the Command window.
- Type the word "PING", space, followed by the IP address and press enter.
- You should see 4 packets of data sent, 4 received, and 0 lost.

If you PING the IP Address successfully (4 sent, 4 received, 0 lost), but still cannot connect, it means that one of the needed ports is still not open. Please contact your IT department and review the instrument checklist.
If you PING the IP address unsuccessfully, please send us the serial number of the instrument so that we can contact your local engineer.

Find additional tips, troubleshooting help, and resources within our Real-Time PCR and Digital PCR Instruments Support Center.

Where can I find the software and newest firmware for the QuantStudio 1 instrument?

In an effort to reduce environmental impact, we no longer supply the software on the CD. Please download it from here (https://www.thermofisher.com/uk/en/home/global/forms/life-science/quantstudio-software-download.html).

Find additional tips, troubleshooting help, and resources within our Real-Time PCR and Digital PCR Instruments Support Center.

Citations & References (962)

Citations & References
Abstract
Comparable type I interferon score determination from PAXgene and Tempus whole blood RNA collection and isolation systems.
Authors:Lamot L,Niemietz I,Brown KL
Journal:BMC research notes
PubMed ID:31416482
OBJECTIVE: Type I interferons (IFN) have important roles in many immune-mediated inflammatory diseases (IMIDs) and are a relatively new therapeutic target. Direct detection of type I IFNs has proved challenging, thus their presence is often inferred from the expression of interferon-stimulated genes (ISGs) and calculation of an interferon score (IS). ... More
Efficient gene editing of human long-term hematopoietic stem cells validated by clonal tracking.
Authors:Ferrari S,Jacob A,Beretta S,Unali G,Albano L,Vavassori V,Cittaro D,Lazarevic D,Brombin C,Cugnata F,Kajaste-Rudnitski A,Merelli I,Genovese P,Naldini L
Journal:Nature biotechnology
PubMed ID:32601433
Targeted gene editing in hematopoietic stem cells (HSCs) is a promising treatment for several diseases. However, the limited efficiency of homology-directed repair (HDR) in HSCs and the unknown impact of the procedure on clonal composition and dynamics upon transplantation have hampered clinical translation. Here, we apply a barcoding strategy to ... More
Tamoxifen induction of Cre recombinase does not cause long-lasting or sexually divergent responses in the CNS epigenome or transcriptome: implications for the design of aging studies.
Authors:Chucair-Elliott AJ,Ocanas SR,Stanford DR,Hadad N,Wronowski B,Otalora L,Stout MB,Freeman WM
Journal:GeroScience
PubMed ID:31493147
The systemic delivery of tamoxifen (Tam) to activate inducible CreERT2-loxP transgenic mouse systems is now widely used in neuroscience studies. This critical technological advancement allows temporal control of DNA-cre recombination, avoidance of embryonically lethal phenotypes, and minimization of residual cell labeling encountered in constitutively active drivers. Despite its advantages, the ... More
CD4(+) CCR6(+) T cells, but not γδ T cells, are important for the IL-23R-dependent progression of antigen-induced inflammatory arthritis in mice.
Authors:Razawy W,Asmawidjaja PS,Mus AM,Salioska N,Davelaar N,Kops N,Oukka M,Alves CH,Lubberts E
Journal:European journal of immunology
PubMed ID:31778214
IL‐23 plays an important role in the development of arthritis and the IL‐23 receptor (IL‐23R) is expressed on different types of T cells. However, it is not fully clear which IL‐23R(+) T cells are critical in driving T cell‐mediated synovitis. We demonstrate, using knock‐in IL‐23R‐GFP reporter (IL‐23R(GFP/+)) mice, that CD4(+)CCR6(+) T cells and γδ ... More
Inactivation of Pepper Mild Mottle Virus in Water by Cold Atmospheric Plasma.
Authors:Filipić A,Dobnik D,Tušek Žnidarič M,Žegura B,Štern A,Primc G,Mozetič M,Ravnikar M,Žel J,Gutierrez Aguirre I
Journal:Frontiers in microbiology
PubMed ID:33584622
Water scarcity is one of the greatest threats for human survival and quality of life, and this is increasingly contributing to the risk of human, animal and plant infections due to waterborne viruses. Viruses are transmitted through polluted water, where they can survive and cause infections even at low concentrations. ... More