BCECF Acid (2',7'-Bis-(2-Carboxyethyl)-5-(and-6)-Carboxyfluorescein), mixed isomers
BCECF Acid (2',7'-Bis-(2-Carboxyethyl)-5-(and-6)-Carboxyfluorescein), mixed isomers
Citations & References (84)
Invitrogen™

BCECF Acid (2',7'-Bis-(2-Carboxyethyl)-5-(and-6)-Carboxyfluorescein), mixed isomers

BCECF is ideal for measuring changes ± 1 pH unit around pH 7, because it is a dual-excitation ratiometric pHRead more
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Catalog NumberQuantity
B1151
also known as B-1151
1 mg
Catalog number B1151
also known as B-1151
Price (KRW)
383,000
Each
Add to cart
Quantity:
1 mg
Price (KRW)
383,000
Each
Add to cart

BCECF is ideal for measuring changes ± 1 pH unit around pH 7, because it is a dual-excitation ratiometric pH indicator with a pKa of ∼6.98. pH measurements with BCECF are made by determining the pH-dependent ratio of emission intensity (detected at 535 nm) when the dye is excited at ∼490 nm versus the emission intensity when excited at its isobestic point of ∼440 nm.

Learn more about ion indicators including calcium, potassium, pH, and membrane potential indicators ›

For Research Use Only. Not for use in diagnostic procedures.
Specifications
Detection MethodFluorescence
Dye TypepH Indicator
Quantity1 mg
Shipping ConditionRoom Temperature
For Use With (Equipment)Microplate Reader
Product TypepH indicator
Unit SizeEach
Contents & Storage
Store at room temperature and protect from light.

Citations & References (84)

Citations & References
Abstract
Measurement of intracellular pH.
Authors:Boyer MJ, Hedley DW
Journal:Methods Cell Biol
PubMed ID:7532259
Fluorescent indicators of ion concentrations.
Authors:Tsien RY
Journal:Methods Cell Biol
PubMed ID:2538708
Mechanistic studies of lantibiotic-induced permeabilization of phospholipid vesicles.
Authors:Driessen AJ, van den Hooven HW, Kuiper W, van de Kamp M, Sahl HG, Konings RN, Konings WN
Journal:Biochemistry
PubMed ID:7849020
'Nisin is a cationic polycyclic bacteriocin secreted by some lactic acid bacteria. Nisin has previously been shown to permeabilize liposomes. The interaction of nisin was analyzed with liposomes prepared of the zwitterionic phosphatidylcholine (PC) and the anionic phosphatidylglycerol (PG). Nisin induces the release of 6-carboxyfluorescein and other small anionic fluorescent ... More
Luminal pH regulated calcium release kinetics in sarcoplasmic reticulum vesicles.
Authors:Donoso P, Beltrán M, Hidalgo C
Journal:Biochemistry
PubMed ID:8873610
'Calcium binding to triads isolated from rabbit skeletal muscle followed a single hyperbolic function in the pH range 5.5-8.0. Maximal binding was obtained at pH 8.0; decreasing the pH decreased the binding capacity and, at pH < or = 6.0, increased Kd 2-fold. These results indicate that lowering the pH ... More
Modulation of the hyperpolarization-activated cation current of rat thalamic relay neurones by intracellular pH.
Authors:Munsch T, Pape HC
Journal:J Physiol
PubMed ID:10457065
'1. Properties of the hyperpolarization-activated cation current (Ih) were investigated in thalamocortical neurones of an in vitro slice preparation of the rat ventrobasal thalamic complex (VB) before and during changes of pipette pH (pHp), intracellular pH (pHi) and bath pH (pHb) using the whole-cell patch-clamp technique and fluorescence ratio imaging ... More
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