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Citations & References (10)
Invitrogen™
Hexidium Iodide
The nucleic acid stain, hexidium iodide is permeant to mammalian cells and selectively stains almost all gram-positive bacteria in theRead more
| Catalog Number | Quantity |
|---|---|
| H7593 | 5 mg |
Catalog number H7593
Price (KRW)
411,000
Online offer
Ends: 31-Dec-2025
483,000Save 72,000 (15%)
Each
Quantity:
5 mg
Price (KRW)
411,000
Online offer
Ends: 31-Dec-2025
483,000Save 72,000 (15%)
Each
The nucleic acid stain, hexidium iodide is permeant to mammalian cells and selectively stains almost all gram-positive bacteria in the presence of gram-negative bacteria. Hexidium iodide exhibits excitation/emission maxima of ∼518/600 nm upon binding DNA. Generally, both the cytoplasm and nuclei of eukaryotic cells show staining with hexidium iodide.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
ColorOrange
Detection MethodFluorescence
Dye TypeCell-Permeant
Excitation Wavelength Range518⁄600
For Use With (Equipment)Fluorescence Microscope
Quantity5 mg
Shipping ConditionRoom Temperature
Label TypeFluorescent Dye
Product TypeNucleic Acid Stain
SubCellular LocalizationCytoplasm, Cytosol, Nucleus
Unit SizeEach
Contents & Storage
Store at room temperature and protect from light.
Citations & References (10)
Citations & References
Abstract
A flow-cytometric gram-staining technique for milk-associated bacteria.
Journal:Appl Environ Microbiol
PubMed ID:12732558
'A Gram-staining technique combining staining with two fluorescent stains, Oregon Green-conjugated wheat germ agglutinin (WGA) and hexidium iodide (HI) followed by flow-cytometric detection is described. WGA stains gram-positive bacteria while HI binds to the DNA of all bacteria after permeabilization by EDTA and incubation at 50 degrees C for 15
A fluorescent Gram stain for flow cytometry and epifluorescence microscopy.
Journal:Appl Environ Microbiol
PubMed ID:9647848
'The fluorescent nucleic acid binding dyes hexidium iodide (HI) and SYTO 13 were used in combination as a Gram stain for unfixed organisms in suspension. HI penetrated gram-positive but not gram-negative organisms, whereas SYTO 13 penetrated both. When the dyes were used together, gram-negative organisms were rendered green fluorescent by
Metabolic activity in filamentous fungi can be analysed by flow cytometry.
Journal:J Microbiol Methods
PubMed ID:12782375
The use of flow cytometry in combination with fluorescent dyes as a technique to rapidly differentiate and enumerate bacterial and yeast cells is well established. We have shown that through the judicial choice of stains, the nondestructive screening and sorting of fungal material is possible. The early stages of growth,
Increased sensitivity of bacterial detection in cerebrospinal fluid by fluorescent staining on low-fluorescence membrane filters.
Journal:J Med Microbiol
PubMed ID:16091435
A membrane-filter-based, fluorescent Gram stain method for bacterial detection in cerebrospinal fluid samples was developed and evaluated as a rapid, sensitive alternative to standard Gram stain protocols. A recently developed, modified version of the aluminium oxide membrane Anopore with low-fluorescence optical properties showed superior performance in this application. Other aspects
Nucleic acid stains as indicators of Cryptosporidium parvum oocyst viability.
Journal:Int J Parasitol
PubMed ID:9279581
We developed nucleic acid dye staining methodology for untreated, heat-treated and chemically inactivated C. parvum oocysts. The nucleic acid staining was compared to in vitro excystation and animal infectivity using split samples of oocysts. Among the nucleic acid stains tested, SYTO-9, hexidium and SYTO-59 stained the oocysts consistently, and the