iBlot™ 3 Starter Kit, low-fluorescence PVDF

The iBlot 3 Starter Kit, Low Fluorescence PVDF, includes the iBlot 3 Western Blot Transfer Device and two boxes of iBlot 3 Transfer Stacks, Midi, Low Fluorescence PVDF—everything needed to perform a rapid dry-transfer of proteins from a gel to a membrane in a fluorescent western blotting experiment.

iBlot 3 Western Blot Transfer Device

The iBlot 3 Western Blot Transfer Device is a dry transfer device that performs western blotting transfer efficiently and reliably in as few as three minutes and without the need for liquid buffers. The iBlot 3 system is compatible with both nitrocellulose, polyvinylidene difluoride (PVDF), and Low Fluorescence PVDF membranes and offers equivalent or better performance compared with traditional wet-transfer methods in a fraction of the time.

The iBlot 3 Western Blot Transfer Device is an integral part of the iBlot dry blotting system, which consists of the transfer device and pre-assembled transfer stacks that contain the required buffers and transfer membrane (nitrocellulose, PVDF, or Low Fluorescence PVDF).

iBlot 3 Western Blot Transfer Device features include:

• Complete protein transfer in as few as three minutes
• High detection sensitivity and even, consistent protein transfer
• Two independently controlled transfer stations capable of transferring up to two midi or four mini blots
• Built-in adjustable cooling to help ensure consistent transfer temperature
• Pre-programed transfer methods optimized for low, high, and broad molecular weight ranges
• Ability to create custom programs
• A simple, user-friendly interface

How the iBlot dry blotting system works

Buffer ion reservoirs are incorporated into the gel matrix of transfer stacks instead of buffer tanks or soaked filter papers. The high density of ions in the gel matrix enables rapid protein transfer. During blotting, the copper anode does not generate oxygen gas as a result of water electrolysis, reducing blot distortion. (Conventional protein transfer techniques, including wet, semi-wet, and semi-dry, use inert electrodes that generate oxygen.)

With the iBlot dry blotting system, transfer time is reduced by the shortened distance between electrodes, high field strength, and high current. Trapped air bubbles, often created during the manual preparation of the blotting sandwich layers, are easy to avoid due to our unique de-bubbling design that promotes even and complete transfer. With the iBlot 3 system, there is no need to prepare buffers or clean up after blotting. The total preparation and run time is normally less than ten minutes per blot.