LIVE/DEAD™ Violet Viability/Vitality Kit, for 405 nm excitation
LIVE/DEAD™ Violet Viability/Vitality Kit, for 405 nm excitation
Invitrogen™

LIVE/DEAD™ Violet Viability/Vitality Kit, for 405 nm excitation

The LIVE/DEAD™ Violet Viability/Vitality Kit provides a two-color fluorescence cell viability and vitality assay that is based on the simultaneousRead more
Have Questions?
Catalog NumberQuantity
L349581 kit
Catalog number L34958
Price (KRW)
968,000
Online offer
Ends: 31-Dec-2025
1,138,000
Save 170,000 (15%)
Each
Add to cart
Quantity:
1 kit
Price (KRW)
968,000
Online offer
Ends: 31-Dec-2025
1,138,000
Save 170,000 (15%)
Each
Add to cart
The LIVE/DEAD™ Violet Viability/Vitality Kit provides a two-color fluorescence cell viability and vitality assay that is based on the simultaneous determination of live and dead cells with two probes that measure recognized parameters of cell health: plasma membrane integrity and intracellular esterase activity. Calcein violet AM and aqua-fluorescent reactive dye are optimal dyes for this application; both dyes utilize the violet laser allowing other laser lines to be used for conventional fluorochromes.

View a selection guide for all Nonfixable Viability Dyes for Flow Cytometry.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Cell PermeabilityImpermeant
Cell TypeEukaryotic Cells
DescriptionLIVE/DEAD™ Violet Viability/Vitality Kit, for 405 nm excitation
Detection MethodFluorescence
Dye TypeOther Label(s) or Dye(s)
FormLyophilized
FormatTube(s)
Quantity1 kit
Shipping ConditionRoom Temperature
SolubilityDMSO (Dimethylsulfoxide)
ColorAqua, Violet
EmissionAqua-fluorescent reactive dye: 367/526, Calcein violet stain: 400/452
Excitation Wavelength Range367/400 nm
For Use With (Application)Viability Assay
For Use With (Equipment)Flow Cytometer
Product LineLIVE/DEAD
Product TypeStain
Unit SizeEach
Contents & Storage
Contains 5 vials of calcein violet stain (25 μg/vial), 5 vials of aqua-fluorescent reactive dye, and 1 vial of DMSO.

Store in freezer (-5°C to -30°C) and protect from light.

Frequently asked questions (FAQs)

How do I prepare dead cell controls for LIVE/DEAD cell viability assays?

There are two easy options. One is to heat-inactivate the cells by placing at 60 degrees C for 20 minutes. The second is to subject the cells to 70% ethanol. Alcohol-fixed cells can be stored indefinitely in the freezer until use, potentially up to several years.

Centrifuge cells, pellet, and remove supernatant.
Fix cells: Add 10 mL ice cold 70% ETOH to a 15 mL tube containing the cell pellet, adding dropwise at first while vortexing, mix well.
Store in freezer until use.
When ready to use, wash twice and resuspend in buffer of choice.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.