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Invitrogen™

BacMam Enhancer Solution (1000X)

The BacMam Enhancer helps to increase expression of BacMam reagents in mammalian cells.To use, simply add the BacMam enhancer toRead more
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Catalog NumberQuantity
PV5835150 μL
Catalog number PV5835
Price (KRW)
143,000
Each
Quantity:
150 μL
Price (KRW)
143,000
Each
The BacMam Enhancer helps to increase expression of BacMam reagents in mammalian cells.

To use, simply add the BacMam enhancer to cell culture media with or without serum that have already been treated with the BacMam reagent, then incubate cells and replace with fresh medium. Different cell types vary in their response to the BacMam enhancer and for best results the amount and treatment time will need to be optimized.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Cell TypeMammalian Cells
Concentration1000 X
DescriptionHistone Deacetylase Inhibitor
For Use With (Application)BacMam-Enabled Cellular Assays
Quantity150 μL
Shipping ConditionDry Ice
FormFrozen
Product TypeBacMam Enhancer Solution
Unit SizeEach
Contents & Storage
Concentration: 1.5 mM (1000X)
Store at -20°C.

Frequently asked questions (FAQs)

I am using your CellLight BacMam reagents for fluorescent protein-labeling of my cell organelles, but they aren't labeling well. How can I improve it?

First, make sure that your cells are mammalian and non-blood derived. If the cells have low transduction efficiency (i.e., only a few cells are labeling or are labeling only dimly), first try increasing the particle number per cell. The next thing to try is to use the BacMam Enhancer Solution. If, however, your cells show labeling, but the localization seems to be too generalized or ubiquitous within the cell, then you should reduce the particle number per cell. The manual gives a guideline and formula for calculating particle number per cell.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.