Salmonella Shigella Agar (Dehydrated)

Perform selective and differential isolation of Salmonella and partly Shigella spp. from clinical specimens, including feces, and food samples¹ using Thermo Scientific™ Remel™ Salmonella Shigella Agar (Dehydrated). Leifson modified the Deoxycholate-Citrate Agar used for the detection of enteric pathogens² and added bile salts mixture to inhibit the growth of gram-positive organisms.

Salmonella and Shigella are major causes of bacterial enteric illness with transmission occurring via the fecal-oral route, person-to-person contact, or by the ingestion of contaminated food or water.

Perform selective and differential isolation of Salmonella and partly Shigella spp. from clinical specimens, including feces, and food samples using Salmonella Shigella Agar.

• Ease of Differentiation: Based on the fermentation of lactose; gram-negative bacilli produce pink to red colonies whereas lactose non-fermenters produce transparent to colorless colonies.
• Selective: Due to the inclusion of selective inhibitory substances brilliant green, bile salts, thiosulphate, and citrate, most gram-positive and coliform organisms are inhibited.

This medium is used for qualitative procedures. This medium contains bile salts, brilliant green and citrates which acts as selective agents and inhibit gram-positive bacteria and coliforms. Sodium thiosulfate, a sulfur source, and ferric ammonium citrate work as indicators to enable organisms which produce H₂S to form black-centered colonies, including some strains of Salmonella. Lactose acts as a differentiator.

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Remel™ and Oxoid™ products are now part of the Thermo Scientific brand.