pLenti6/V5-DEST™ Gateway™ Vector

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Citations & References (4)

Invitrogen™

pLenti6/V5-DEST™ Gateway™ Vector

The pLenti6⁄V5-DEST™ Gateway™ Vector is a Gateway™-adapted ViraPower™ lentiviral expression vector for lentiviral-based expression of a target gene in dividingRead more

Catalog Number	Quantity
V49610	6 μg

Catalog number V49610

Price (KRW)

2,691,000

Online offer

Ends: 31-Dec-2025

3,165,000

Save 474,000 (15%)

Add to cart

Quantity:

Price (KRW)

2,691,000

Online offer

Ends: 31-Dec-2025

3,165,000

Save 474,000 (15%)

Add to cart

The pLenti6⁄V5-DEST™ Gateway™ Vector is a Gateway™-adapted ViraPower™ lentiviral expression vector for lentiviral-based expression of a target gene in dividing and non-dividing mammalian cells. The vector has the CMV promoter for driving constitutive expression of the target gene and the blasticidin selection marker for stable selection in mammalian cells.

Advantages
• Lentivirus based expression of a target gene in dividing and non-dividing mammalian cells

Key Features
• Flexible and versatile Gateway™ recombination cloning technology
• Constitutive high expression with CMV promoter
• Blasticidin selection marker for stable selection
• C terminal V5 tag for quick detection

Kit includes
• pLenti6⁄V5-DEST™ Gateway™ Vector
• One Shot™ Stbl3™ Chemically Competent E. coli (C7373-03)

Related SKUs
• pLenti6⁄UbC⁄V5-DEST™ Gateway™ Vector (V49910)
• pLenti4⁄V5-DEST™ Gateway™ Vector (V49810)
• pLenti6⁄V5 Directional TOPO™ Cloning Kit (K4955-10)
• ViraPower™ Lentiviral Directional TOPO™ Expression Kit (K495000)
• ViraPower™ Lentiviral Gateway™ Expression Kit (K4960-00)
• ViraPower™ HiPerform™ Lentiviral Gateway™ Expression Kit (K5330-00)

For research use only. Not intended for any therapeutic or diagnostic use.

For Research Use Only. Not for use in diagnostic procedures.

Specifications

Constitutive or Inducible SystemConstitutive

Delivery TypeLentiviral

For Use With (Application)Viral Expression

Product TypeLentiviral Expression Vector

Quantity6 μg

Selection Agent (Eukaryotic)Blasticidin

VectorpDEST, pLenti

Cloning MethodGateway

Product LineGateway, ViraPower

PromoterCMV

Protein TagV5 Epitope Tag

Unit Size6 µg

Contents & Storage

ViraPower™ Lentiviral Gateway™ Vectors, store at –20°C includes:

pLenti6⁄V5-DEST™: 6 μg (40 μl of 150 ng⁄μl vector in 10 mM
Tris-HCl, 1mM EDTA, pH 8.0)

pLenti6⁄V5-GW⁄lacZ control: 10 μg
(20 μl of 0.5 μg⁄μl vector in 10 mM
Tris-HCl, 1mM EDTA, pH 8.0)

One Shot™ Stbl3™ Chemically Competent E. coli store at –80°C

Frequently asked questions (FAQs)

Can I perform the single-step protocol for the BP/LR Clonase reaction using BP Clonase enzyme and LR Clonase enzyme instead of BP Clonase II enzyme and LR Clonase II enzyme?

In the single-step protocol for the BP/LR Clonase reaction, we would not recommend substituting the BP Clonase II/LR Clonase II enzymes with BP Clonase /LR Clonase enzymes as this would result in very low recombination efficiency.

Do you have a recommended single-step protocol for BP/LR recombination?

Yes, we have come up with a single-step protocol for BP/LR Clonase reaction (http://www.thermofisher.com/us/en/home/life-science/cloning/gateway-cloning.html#1), where DNA fragments can be cloned into Destination vectors in a single step reaction, allowing you to save time and money.

How can I move my gene of interest from a Gateway-adapted expression clone to a new Destination vector as I have lost the entry clone?

We would recommend performing a BP reaction with a Donor vector in order to obtain an entry clone. This entry clone can then be used in an LR reaction with the Destination vector to obtain the new expression clone.

Can I purchase the 5X LR Clonase buffer or 5X BP Clonase buffer separately?

We do not offer the 5X LR Clonase buffer and 5X BP Clonase buffer as standalone products. They are available as part of the enzyme kits.

Do you offer Gateway vectors for expression in plants?

We do not offer any Gateway vectors for expression in plants.

View all pLenti6/V5-DEST™ Gateway™ Vector FAQs

Citations & References (4)

Citations & References

Abstract

The membrane anchor R7BP controls the proteolytic stability of the striatal specific RGS protein, RGS9-2.

Authors:Anderson GR, Semenov A, Song JH, Martemyanov KA,

Journal:J Biol Chem

PubMed ID:17158100

'A member of the RGS (regulators of G protein signaling) family, RGS9-2 is a critical regulator of G protein signaling pathways that control locomotion and reward signaling in the brain. RGS9-2 is specifically expressed in striatal neurons where it forms complexes with its newly discovered partner, R7BP (R7 family binding ... More

West Nile virus discriminates between DC-SIGN and DC-SIGNR for cellular attachment and infection.

Authors:Davis CW, Nguyen HY, Hanna SL, Sánchez MD, Doms RW, Pierson TC,

Journal:J Virol

PubMed ID:16415006

'The C-type lectins DC-SIGN and DC-SIGNR bind mannose-rich glycans with high affinity. In vitro, cells expressing these attachment factors efficiently capture, and are infected by, a diverse array of appropriately glycosylated pathogens, including dengue virus. In this study, we investigated whether these lectins could enhance cellular infection by West Nile ... More

Tumorigenesis suppressor Pdcd4 down-regulates mitogen-activated protein kinase kinase kinase kinase 1 expression to suppress colon carcinoma cell invasion.

Authors:Yang HS, Matthews CP, Clair T, Wang Q, Baker AR, Li CC, Tan TH, Colburn NH,

Journal:Mol Cell Biol

PubMed ID:16449643

Programmed cell death 4 (Pdcd4) suppresses neoplastic transformation by inhibiting the activation of c-Jun and consequently AP-1-dependent transcription. We report that Pdcd4 blocks c-Jun activation by inhibiting the expression of mitogen-activated protein kinase kinase kinase kinase 1 (MAP4K1)/hematopoietic progenitor kinase 1, a kinase upstream of Jun N-terminal kinase (JNK). cDNA ... More

Isolation of cell lines that show novel, murine leukemia virus-specific blocks to early steps of retroviral replication.

Authors:Bruce JW, Bradley KA, Ahlquist P, Young JA,

Journal:J Virol

PubMed ID:16188999

In order to identify cellular proteins required for early stages of retroviral replication, a high volume screening with mammalian somatic cells was performed. Ten pools of chemically mutagenized Chinese hamster ovary (CHO-K1) cells were challenged with a murine leukemia virus (MLV) vector pseudotyped with the vesicular stomatitis virus glycoprotein (VSV-G), ... More