Does the eBioscience Foxp3/Transcription Factor Staining Buffer Set (Cat. No. 00-5523-00) contain formaldehyde?
One of the components of the eBioscience Foxp3/Transcription Factor Staining Buffer Set (Cat. No. 00-5523-00) is the Fixation/Permeabilization Concentrate (Cat. No. 00-5123-43). The Fixation/Permeabilization Concentrate contains formaldehyde.
Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.
Can I use the Foxp3 / Transcription Factor Staining Buffer Set to process samples for imaging?
The Foxp3 / Transcription Factor Staining Buffer Set was optimized for processing samples for flow cytometry; it may work for samples for imaging analysis, but we have not tested this.
Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.
Can I get the identity/formulation for the individual components in the Foxp3 / Transcription Factor Staining Buffer Set?
The fixative is formaldehyde, the identity and formulation of other kit components are proprietary.
Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.
What is shipped with the Foxp3 / Transcription Factor Staining Buffer Set and what is their shelf life and storage conditions?
The Foxp3 / Transcription Factor Staining Buffer Set includes 3 bottles:
- Fixation/Permeabilization Concentrate (4X) (30 mL)
- Fixation/Permeabilization Diluent (100 mL)
- Permeabilization Buffer (100 mL)
They should be stored at 2-8 degrees C and should be used within 6 months.
Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.
Can I use the eBioscience Foxp3/Transcription Factor Staining Buffer Set (Cat. No. 00-5523-00) with the Blimp1-PE antibody from another company?
Yes, the eBioscience Foxp3/Transcription Factor Staining Buffer Set will allow any antibody to reach an intra-nuclear target to stain cells, including antibodies from other vendors.
Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.
