123count eBeads™ Counting Beads

123count eBeads are intended for use in absolute counting of cells or other particles by flow cytometry. These are 7자세히 알아보기

카탈로그 번호	수량
01-1234-42	100 tests

카탈로그 번호 01-1234-42

제품 가격(KRW)

344,000

Online offer

Ends: 31-Mar-2026

404,000

할인액 60,000 (15%)

카트에 추가하기

100 tests

제품 가격(KRW)

344,000

Online offer

Ends: 31-Mar-2026

404,000

할인액 60,000 (15%)

카트에 추가하기

123count eBeads are intended for use in absolute counting of cells or other particles by flow cytometry. These are 7 μm microparticles containing encapsulated dyes compatible with blue (488 nm) and violet (405 nm) excitation sources and emitting fluorescence between approximately 500 nm and 750 nm. They are supplied at a known concentration in 0.05% Tween-20 with 2 mM sodium azide. Collecting carefully suspended and measured quantities of cell:bead mixtures on a flow cytometer makes the ratiometric enumeration of cells possible.

Reported Application
Flow Cytometric Analysis

For Research Use Only. Not for use in diagnostic procedures.

용도(장비)Flow Cytometer

테스트 수100 tests

수량100 tests

제품라인eBeads

제품 유형Cell Counting Bead

Unit SizeEach

자주 묻는 질문(FAQ)

I am using counting beads to count cells, but I cannot find the beads on my scatter plots. What do I do?

The first thing to do is check your threshold and see if it is set on forward scatter. If so, the beads are probably being excluded by the threshold. Reducing the threshold setting should reveal your beads.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

I want to count my cells using flow cytometry. How do I do this?

Cell counting using flow cytometry can be accomplished by adding an internal microsphere counting standard to the flow cytometric sample. The number of reference beads that are collected reflects a known volume. This allows you to calculate cell concentration.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

What does yield and purity mean in reference to the MagniSort kits? How are each of these parameters calculated?

Yield is the percentage of target cells harvested based on the number of cells theoretically available in the starting population. To calculate yield, stain a sample of unsorted and sorted cells with antibodies to identify the target cells, and count the total number of target cells using 123count eBeads (Cat. No. 01-1234-42) on the cytometer. Yield = # of sorted target cells, # of target cells unsorted.

Purity is the percentage of cells harvested that are target cells. Purity can be directly generated by flow cytometric analysis of sorted samples. To obtain purity, stain the sorted cells with antibodies to identify the target cells and analyze by flow cytometry. Gate on live, single cells, and subsequently gate on the target cell population. Purity is the percentage of target cells in the live, single-cell gate.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.