PBS, pH 7.4
PBS, pH 7.4
인용 및 참조 문헌 (6)
Gibco™

PBS, pH 7.4

PBS(phosphate buffered saline)는 해리 전 세포 세척, 세포 또는 조직의 이동, 계수 전 세포 희석, 시약 조제 등 다양한 세포자세히 알아보기
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카탈로그 번호수량
10010023500 mL
100100311000 mL
1001004910 x 500 mL
100100726 x 1000 mL
카탈로그 번호 10010023
제품 가격(KRW)
44,000
Online offer
Ends: 31-Dec-2025
46,000
할인액 2,000 (4%)
Each
카트에 추가하기
수량:
500 mL
제품 가격(KRW)
44,000
Online offer
Ends: 31-Dec-2025
46,000
할인액 2,000 (4%)
Each
카트에 추가하기
PBS(phosphate buffered saline)는 해리 전 세포 세척, 세포 또는 조직의 이동, 계수 전 세포 희석, 시약 조제 등 다양한 세포 배양 응용 분야에 사용하는 평형 염액(balanced salt solution)입니다. PBS는 칼슘과 마그네슘 없이 제조되므로 세포 해리 전 배양물로부터 킬레이터를 린스(rinse)하는데 사용될 수 있습니다. 당사는 광범위한 세포 배양 응용 분야에 맞는 다양한 조성의 PBS를 제공합니다. 배지 선택 도구를 사용하면 알맞는 조성을 찾을 수 있습니다..

이 PBS는 다음과 같이 제조됩니다.
제외 성분
• 칼슘
• 마그네슘
• 페놀 레드(phenol red)

전체 조성 이용 가능.

cGMP 제조 및 품질 시스템
PBS는 영국 스코틀랜드의 Paisley에 위치한 cGMP 규정 준수 시설에서 제조됩니다. 이 시설은 의료기기 제조업체로 FDA에 등록되어 있으며 ISO 13485 스탠더드 인증을 받았습니다. 공급망 유지를 위해 당사는 또한 Grand Island 시설에서 동일한 PBS 제품을 제조하여 공급합니다 (10010056). 이 시설도 또한 의료기기 제조업체로 FDA에 등록되어 있으며 ISO 13485 스탠더드 인증을 받았습니다.

This product is not used for in vitro diagnostic purpose in some countries.
For Research Use or Further Manufacturing. Not for diagnostic use or direct administration into humans or animals.
사양
화학물질 이름 또는 재질Phosphate Buffered Saline (PBS)
색상Clear
포함No Calcium, No Magnesium
제조 품질cGMP-compliant under the ISO 13485 standard
삼투압280 - 315 mOsm/kg
포장Bottle
권장 스토리지Storage conditions: 15°C to 30°C
Shipping conditions: Room temperature
Shelf life: 24 months from date of manufacture
멸균Sterile-filtered
농도1 X
용도(애플리케이션)Washing Cells Before Dissociation; Diluting Cells for Counting and Preparing Reagents; Transporting Cells or Tissue
물리적 형태Liquid
제품라인Gibco
수량500 mL
용액 유형Phosphate Buffered Saline
pH7.4
Unit SizeEach

자주 묻는 질문(FAQ)

How can I make isolation buffer for cell isolation?

To prepare this buffer:

(1) Make up 100 mL of a stock solution of 0.5 M EDTA by dissolving 14.6 g EDTA in approximately 80 mL of ddH2O. Adjust the pH to 7.4, then adjust the volume to 100 mL. Autoclave and filter 25 mL aliquots through a sterilized filter into 4 sterile 50 mL tubes. Store one at 4 degrees C and the rest at -20 degrees C for future use.
(2) Make up a stock solution of 20% BSA by putting 5 g of BSA in a 50 mL tube and filling it with ddH2O up to 25 mL. Leave on a roller at room temperature until dissolved. Filter through a sterile 0.2 µm filter into a sterile 50 mL tube and adjust the volume to 30 mL with sterile H2O.
(3) Make the isolation buffer by taking a 500 mL bottle of 1x PBS, pH 7.4 without Ca2+ or Mg2+ (Cat. No. 10010023). Add 2.5 mL 20% BSA and 2 mL 0.5 M EDTA. Mix by turning the bottle end over end 10 times. Store at 4 degrees C.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

My PBS, PH 7.4 (Cat. No. 10010072) was accidentally frozen. Can I still use it?

This product should be stored at 2-8 degrees C and should not be frozen. The biggest issue with accidentally freezing this product is its solubility. If this product was accidentally frozen, we recommend placing it in a 2-8 degree environment and allowing it to slowly thaw overnight. If the product is fully thawed and shows no signs of precipitation, then it should still be usable, but we cannot guarantee effectiveness.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Are your PBS products tested for endotoxin levels?

Our PBS, pH 7.2 and PBS, pH 7.4 products are tested for endotoxin and the endotoxin levels are shown on the Certificate of Analysis (CoA) for the particular lot number of the product.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Are your PBS products sterile and suitable for cell culture?

We offer PBS - Phosphate-Buffered Saline (10X) pH 7.4, RNase-free (Cat. Nos. AM9624 and AM9625), which is molecular biology grade and is certified to be RNase-free. However, it is not sterile and hence is not cell culture-grade.

All our other PBS products (Cat. Nos. 70011xxx, 10010xxx, 70013xxx, 20012xxx) are filter-sterilized using a 0.1 µm filter and are cell culture grade (ready to use directly on cells). However, they are not tested for nuclease activity.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Are your PBS products DNase- and RNase-free?

We offer PBS - Phosphate-Buffered Saline (10X) pH 7.4, RNase-free (Cat. Nos. AM9624 and AM9625) that is molecular biology grade and is certified to be RNase-free. Our other PBS products are made with distilled water and are filter-sterilized, but are not tested for RNase and DNase activity.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

인용 및 참조 문헌 (6)

인용 및 참조 문헌
Abstract
A comprehensive protocol for efficient differentiation of human NPCs into electrically competent neurons.
Authors:Romito E,Battistella I,Plakhova V,Paplekaj A,Forastieri C,Toffolo E,Musio C,Conti L,Battaglioli E,Rusconi F
Journal:Journal of neuroscience methods
PubMed ID:39053772
Aldose reductase mediates mitogenic signaling in vascular smooth muscle cells.
Authors: Ramana Kota V; Chandra Deepak; Srivastava Sanjay; Bhatnagar Aruni; Aggarwal Bharat B; Srivastava Satish K;
Journal:J Biol Chem
PubMed ID:12063254
'Abnormal vascular smooth muscle cell (VSMC) proliferation is a key feature of atherosclerosis and restenosis; however, the mechanisms regulating growth remain unclear. Herein we show that inhibition of the aldehyde-metabolizing enzyme aldose reductase (AR) inhibits NF-kappa B activation during restenosis of balloon-injured rat carotid arteries as well as VSMC proliferation ... More
Blocking HES1 expression initiates GABAergic differentiation and induces the expression of p21(CIP1/WAF1) in human neural stem cells.
Authors: Kabos Peter; Kabosova Andrea; Neuman Toomas;
Journal:J Biol Chem
PubMed ID:11809764
Mammalian neural stem cells can develop into a variety of neuronal and glial cell types. This involves a highly coordinated process of differentiation in which the Notch signaling pathway and the system of helix-loop-helix (HLH) transcriptional regulators play a key role. By exercising control over proliferation, initiation of differentiation, neurite ... More
Cholesterol modulates the membrane binding and intracellular distribution of annexin 6.
Authors: de Diego Iñaki; Schwartz Felix; Siegfried Heide; Dauterstedt Paul; Heeren Joerg; Beisiegel Ulrike; Enrich Carlos; Grewal Thomas;
Journal:J Biol Chem
PubMed ID:12070178
Annexins are Ca(2+)- and phospholipid-binding proteins that are widely expressed in mammalian tissues and that bind to different cellular membranes. In recent years its role in membrane traffic has emerged as one of its predominant functions, but the regulation of its intracellular distribution still remains unclear. We demonstrated that annexin ... More
A 2-D liquid separations/mass mapping method for interlysate comparison of ovarian cancers.
Authors: Kachman Maureen T; Wang Haixing; Schwartz Donald R; Cho Kathleen R; Lubman David M;
Journal:Anal Chem
PubMed ID:11985308
A two-dimensional liquid phase separation of proteins from whole cell lysates coupled on-line to an electrospray-ionization time-of-flight (ESI-TOF) mass spectrometer (MS) is used to map the protein content of ovarian surface epithelial cells (OSE) and an ovarian carcinoma-derived cell line (ES2). The two dimensions involve the use of liquid isoelectric ... More
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