Taq DNA Polymerase, recombinant
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<i>Taq</i> DNA Polymerase, recombinant
<i>Taq</i> DNA Polymerase, recombinant
<i>Taq</i> DNA Polymerase, recombinant
Invitrogen™

Taq DNA Polymerase, recombinant

Taq DNA Polymerase is a thermostable enzyme that synthesizes DNA from single-stranded templates in the presence of dNTPs and a primer.
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카탈로그 번호반응 수
103421785000 Reactions
10342053100 Reactions
10342020500 Reactions
카탈로그 번호 10342178
제품 가격(KRW)
2,558,000
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Ends: 31-Dec-2025
3,009,000
할인액 451,000 (15%)
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반응 수:
5000 Reactions
대량 주문 또는 맞춤형 요청
제품 가격(KRW)
2,558,000
Online offer
Ends: 31-Dec-2025
3,009,000
할인액 451,000 (15%)
Each
카트에 추가하기
Taq DNA Polymerase, recombinant, is a thermostable enzyme that synthesizes DNA from single-stranded templates in the presence of dNTPs and a primer. This recombinant enzyme is purified from the cloned Thermus aquaticus DNA polymerase gene expressed in E. coli and consists of a single polypeptide with a molecular weight of 94 kDa. It has a 5' to 3' DNA polymerase activity and a 5' to 3' exonuclease activity.

Features

  • A choice of recombinant or native enzyme
  • Amplification of PCR products up to 5 kb in size
  • Enzyme is licensed and qualified for PCR

Applications

  • Amplification of DNA from complex genomic, viral, and plasmid templates
  • RT-PCR
  • Sequencing ssDNA
  • Cycle sequencing

Notes

  • One unit of Taq DNA Polymerase is the amount of enzyme required to incorporate 10 nmoles of deoxyribonucleotide into DNA in 30 minutes at 74°C.
  • For superior PCR performance, DreamTaq DNA Polymerase is recommended.
For Research Use Only. Not for use in diagnostic procedures.
사양
엑소뉴클레아제 활성5' - 3'
Fidelity (Taq 대비)1X
형식Stand-alone enzyme
핫 스타트No
반응 수5000 Reactions
오버행3'-A
중합효소Taq DNA Polymerase
제품 유형DNA Polymerase
수량5000 Units
반응 형식Separate Components
배송 조건Dry Ice
크기(최종 제품)5 kb or less
시작 물질DNA
농도5 U/μL
용도(애플리케이션)Standard PCR
GC-Rich PCR PerformanceLow
PCR 방법qPCR, Standard PCR
반응 속도Standard
Unit SizeEach
구성 및 보관
• Taq DNA Polymerase (5 U/μL), 1000 μL
• 10X PCR buffer, 20 mL
• 50 mM MgCl2, 10 mL

Store at -10°C to -30°C in a non-frost-free freezer.
Guaranteed stable for 6 months when stored properly.

자주 묻는 질문(FAQ)

My oligonucleotide does not appear to be the right length when I checked by gel electrophoresis. Why is this?

Oligos should be run on a polyacrylamide gel containing 7 M urea and loaded with a 50% formamide solution to avoid compressions and secondary structures. Oligos of the same length and different compositions can electrophorese differently. dC's migrate fastest, followed by dA's, dT's, and then dG's. Oligos containing N's tend to run as a blurry band and generally have a problem with secondary structure.

The primers I am using worked for PCR initially, but over time, have stopped working. What happened?

Primers should be aliquoted for single use before PCR set-up. Heat just the aliquoted primers to 94 degrees for 1 min. Quick chill the primer on ice before adding to the PCR reaction. Some primers may anneal to themselves or curl up on themselves.

I don't see a pellet in my oligo tube order. Should I ask for a replacement?

The drying method dries the primer in a thin layer along the sidewalls of the tube instead of the bottom, therefore a pellet is not always visible and should still be ready to use.

There is a ball-shaped pellet at the bottom of my oligo tube. What is this and can I still use my oligo?

If the oligo was overheated, it will appear as a “ball”-shaped pellet attached to the bottom of the tube. This should not affect the quality of the oligo, and the oligo should be readily soluble in water.

There is a green color in my lyophilized oligo. Can I still use it?

If an oligo appears green in color, this is most likely due to ink falling into the tube. The oligo should still be fully functional. The color can be removed by doing an ethanol precipitation.

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