MAX Efficiency™ DH10Bac Competent Cells

본 제품은 LMO 제품으로, 고객 분께서 LMO 신고 시스템을 통해 직접 수입 신고를 진행해주셔야 합니다. 자세히보기

인용 및 참조 문헌 (4)

Gibco™

MAX Efficiency™ DH10Bac Competent Cells

MAX Efficiency DH10Bac Competent Cells are used for production of recombinant bacmids used in the Bac-to-Bac Baculovirus Expression System. The자세히 알아보기

카탈로그 번호	수량
10361012	5 x 100 μL

카탈로그 번호 10361012

제품 가격(KRW)

518,000

Exklusiv online

Ends: 31-Mar-2026

575,000

할인액 57,000 (10%)

카트에 추가하기

5 x 100 μL

제품 가격(KRW)

518,000

Exklusiv online

Ends: 31-Mar-2026

575,000

할인액 57,000 (10%)

카트에 추가하기

MAX Efficiency DH10Bac Competent Cells are used for production of recombinant bacmids used in the Bac-to-Bac Baculovirus Expression System. The DH10Bac E. coli strain contains a baculovirus shuttle vector (bacmid) that can recombine with a donor plasmid, pFastBac, to create an expression bacmid containing a cloned gene of interest. Features of MAX Efficiency DH10Bac Competent Cells include:

• Consistent yields of >1 × 10⁸ transformants/μg
• Tolerance of small amounts of undiluted ligation reactions
• Support of efficient blue/white colony screening (Φ80lacZΔM15 marker)

Using MAX Efficiency DH10Bac competent cells
MAX Efficiency DH10Bac Competent Cells can serve as a host for a recombinant pFastBac vector containing a cloned gene of interest. DH10Bac cells harbor a baculovirus shuttle vector (bMON14272) and a helper plasmid (pMON7142), and are capable of supporting site-specific recombination between pFastBac and bMON14272 to generate high molecular weight bacmids that can then be amplified, purified, and used for insect cell transfection and subsequent gene expression. Kanamycin resistance for bacmid selection and maintenance is conferred by bMON14272, and tetracycline resistance by pMON7124.

Genotype:F^-mcrA Δ(mrr-hsdRMS-mcrBC) Φ80lacZΔM15 ΔlacX74 recA1 endA1 araD139 Δ(ara, leu)7697 galU galK λ^-rpsL nupG/pMON14272/pMON7124

For Research Use Only. Not for use in diagnostic procedures.

박테리아 또는 효모 균주DH10Bac

블루/화이트 스크리닝Yes

세포 유형Chemically Competent

메틸화 DNA 클로닝No

불안정 DNA 클로닝Not Suitable for cloning unstable DNA

에프에피솜 포함Lacks F' Episome

효율> 1x10^8

고처리량 호환성Not High-throughput Compatible (Manual)

플라스미드 품질 개선No

비메틸화 DNA 준비Not Suitable for preparing unmethylated DNA

제품라인DH10Bac, MAX Efficiency

제품 유형Competent Cells

ccdB 벡터 증식Not for ccdB vector propagation

수량5 x 100 μL

재조합 감소No

배송 조건Dry Ice

T1 Phage - 저항성(tonA)No

형질전환 효율 수준Medium Efficiency (10^8-10^9 cfu⁄μg)

형식Tube

종E. coli

Unit SizeEach

구성 및 보관

Contains:
• MAX Efficiency DH10Bac Chemically Competent Cells: 5 vials, 100 μl each (total of 500 μl)
• pUC19 DNA (0.01 μg/ml): 1 vial, 100 μl
• SOC Medium: 2 bottles, 6 ml each

Store Competent Cells at -80°C. Store pUC19 DNA at -20°C. Store SOC Medium at 4°C or at room temperature.

자주 묻는 질문(FAQ)

I cannot detect any recombinant fusion protein after using the BaculoDirect Expression Kit. What could be the cause for this and what do you suggest I try?

Please check the construction of your entry clone, and ensure that the insert is in frame with the vector. Analyze the recombinant viral DNA by PCR to confirm the correct size and orientation of your insert after the LR reaction. Sequence your PCR product to verify the proper reading frame for expression of the epitope tag.

Find additional tips, troubleshooting help, and resources within our Protein Expression Support Center.

I'm getting a low-titer P1 viral stock and would like to generate a high-titer stock. What should I do?

To get a high-titer stock, reinfect cells with the P1 stock and generate a P2 high-titer stock. Follow the directions in the BaculoDirect manual on page 18 to generate your P2 stock.

Find additional tips, troubleshooting help, and resources within our Protein Expression Support Center.

I performed an LR reaction, followed by transfection into Sf9/Sf21 insect cells, but do not see any signs of infection even though it's been 72 hours. What should i do?

Please see our recommendations below:

- Check the LR reaction by PCR analysis prior to transfection into insect cells.
- We recommend using Grace's Insect Cell Culture Medium, Unsupplemented during the transfection experiment instead of serum-free medium, as components in serum-free medium may interfere with transfection.
- Ensure that FBS, supplements, or antibiotics are not included during transfection, as the proteins in these materials can interfere with the Cellfectin II Reagent.
- Use the LR recombination reaction using the pENTR/CAT plasmid as a positive control and Cellfectin II Reagent only (mock transfection) as a negative control.
- Ensure that cells are in the log phase of growth with >95% viability, and the amount of cells are in accordance with the suggestions in the manual.
- Cells may not show signs of viral infection for up to a week depending on transfection efficiency; continue culturing and monitor cells daily for signs of infection.

I see a precipitate in my ganciclovir solution. What can I do?

Warm the ganciclovir solution in a water bath at 37 degrees C for 5-10 min, then vortex for a few minutes. The precipitate should go back into solution.

Find additional tips, troubleshooting help, and resources within our Protein Expression Support Center.

I am purifying my secreted protein expressed in insect cells with a His-tagged purification column and getting no yield of my protein. Why and what can I do?

Media used to culture insect cells usually have an acidic pH (6.0-6.5) or contain electron-donating groups that can prevent binding of the 6xHis-tagged protein to Ni-NTA. Amino acids such as glutamine, glycine, or histidine are present at significantly higher concentrations in media for growing insect cells than in media for growing mammalian cells, and compete with the 6xHis-tagged protein for binding sites on Ni-NTA matrices. Grace's medium (Thermo Fisher Scientific), for example, contains approximately 10 mM glutamine, 10 mM glycine, and 15 mM histidine.

Dialysis of the medium against a buffer with the appropriate composition and pH (8.0) similar to the lysis buffer recommended for purification under native conditions usually restores optimal binding conditions. Note that depending on the medium used, a white precipitate (probably made up of insoluble salts) can occur, but normally the 6xHis-tagged protein remains in solution. This can be tested by either protein quantitation if using a protein-free medium or by monitoring the amount of 6xHis-tagged protein by western-blot analysis. After centrifugation, 6xHis-tagged protein can be directly purified from the cleared supernatant.

Find additional tips, troubleshooting help, and resources within our Protein Expression Support Center.

View all MAX Efficiency™ DH10Bac Competent Cells FAQs

인용 및 참조 문헌 (4)

인용 및 참조 문헌

Abstract

Molecular characterization of hCTR1, the human copper uptake protein.

Authors: Eisses John F; Kaplan Jack H;

Journal:J Biol Chem

PubMed ID:12034741

'We have expressed hCTR1, the human copper transporter, in Sf9 cells using a baculovirus-mediated expression system, and we observed greatly enhanced copper uptake. Western blots showed that the protein is delivered to the plasma membrane, where it mediates saturable copper uptake with a K(m) of approximately 3.5 microm. We also ... More

Characterization of a heparan sulfate 3-O-sulfotransferase-5, an enzyme synthesizing a tetrasulfated disaccharide.

Authors:Mochizuki H, Yoshida K, Gotoh M, Sugioka S, Kikuchi N, Kwon YD, Tawada A, Maeyama K, Inaba N, Hiruma T, Kimata K, Narimatsu H,

Journal:J Biol Chem

PubMed ID:12740361

Heparan sulfate d-glucosaminyl 3-O-sulfotransferases (3-OSTs) catalyze the transfer of sulfate from 3'-phosphoadenosine 5'-phosphosulfate (PAPS) to position 3 of the glucosamine residue of heparan sulfate and heparin. A sixth member of the human 3-OST family, named 3-OST-5, was recently reported (Xia, G., Chen, J., Tiwari, V., Ju, W., Li, J.-P., Malmstrom, ... More

Gene characterized for membrane desaturase that produces (E)-11 isomers of mono- and diunsaturated fatty acids.

Authors: Liu Weitian; Jiao Hongmei; Murray Nancy C; O'Connor Marion; Roelofs Wendell L;

Journal:Proc Natl Acad Sci U S A

PubMed ID:11805319

Moth species have evolved integral membrane desaturases that exhibit a wide diversity in substrate specificity, as well as in regiospecificity and stereospecificity of the unsaturated products. We report here the cloning and expression of a single desaturase from the sex pheromone gland of the light brown apple moth, Epiphyas postvittana, ... More

Reconstitution of ATP-dependent leukotriene C4 transport by Co-expression of both half-molecules of human multidrug resistance protein in insect cells.

Authors: Gao M; Loe D W; Grant C E; Cole S P; Deeley R G;

Journal:J Biol Chem

PubMed ID:8910374

Multidrug resistance protein (MRP) confers a multidrug resistance phenotype similar to that associated with overexpression of P-glycoprotein. Unlike P-glycoprotein, MRP has also been shown to be a primary active ATP-dependent transporter of conjugated organic anions. The mechanism(s) by which MRP transports these compounds and increases resistance to natural product drugs ... More