Cellfectin™ II Reagent
Cellfectin™ II Reagent
Gibco™

Cellfectin™ II Reagent

Cellfectin™ II Reagent는 최적의 곤충 세포 transfection을 위한 양이온 지질 제형입니다. Cellfectin™ II는 원래 Cellfectin™ Reagent에서 개선된 버전입니다. Cellfectin™ II는자세히 알아보기
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카탈로그 번호수량
103621001 mL
카탈로그 번호 10362100
제품 가격(KRW)
589,000
Online offer
Ends: 31-Dec-2025
654,000
할인액 65,000 (10%)
Each
카트에 추가하기
수량:
1 mL
제품 가격(KRW)
589,000
Online offer
Ends: 31-Dec-2025
654,000
할인액 65,000 (10%)
Each
카트에 추가하기
Cellfectin™ II Reagent는 최적의 곤충 세포 transfection을 위한 양이온 지질 제형입니다. Cellfectin™ II는 원래 Cellfectin™ Reagent에서 개선된 버전입니다. Cellfectin™ II는 Cellfectin™와 동일한 우수한 성능을 제공하는 것 외에도 로트 간 일관성과 신속한 프로토콜을 보장합니다. BaculoDirect™ and InsectSelect™ Expression Systems을 사용해 Transfection을 할 때 Cellfectin™ II Reagent로 일관적이고 효율적인 Sf9, Sf21, High Five™ 세포 transfection을 할 수 있습니다. Cellfectin™ II Reagent는 혈청 함유 또는 무혈청 배지에서 부착 또는 현탁 포유류 세포 transfection에도 사용할 수 있습니다.

본 제품은 냉장/냉동제품으로 반송된 제품은 전량 폐기 처리 되오니 주문 전 상세 내용 다시 한번 확인 부탁드립니다.
For Research Use Only. Not for use in diagnostic procedures.
사양
용도(애플리케이션)Protein Expression and Transfection
용도(장비)BaculoDirect and InsectSelect Expression Systems
고처리량 호환성Not High-throughput Compatible (Manual)
제품라인Cellfectin
제품 유형Reagent
수량1 mL
혈청 호환 가능No
배송 조건Wet Ice
셀 유형Insect Cells
샘플 종류Baculovirus DNA, Plasmid DNA
Transfection TechniqueLipid-based Transfection
Unit SizeEach
구성 및 보관
Contains one tube (1 ml) Cellfectin™ II Reagent. Store at 4°C. Do not freeze.

자주 묻는 질문(FAQ)

Both transfected cells and Cellfectin II Reagent alone negative control cells are dead within 36 hours. What could cause this?

There are several possibilities:

- Using media containing antibiotics during transfections.
- Plating cells at too low a density: We recommend at least 70% confluence.
- Using cells at too early a passage: We recommend growing cells for at least 5 passages before using them for transfection.
- Contamination because of no pen/strep after the transfection: After 5-8 hr incubation with the transfection mixture, remove the mixture and add antibiotics containing media/well.

Find additional tips, troubleshooting help, and resources within our Transfection Support Center.

My transfected cells are floating up and look dead, but the Cellfectin II Reagent alone control plate is fine. What could be happening?

This may be due to contamination or cytotoxicity from the bacmid prep. Make sure to include a negative control that is the bacmid only without Cellfectin II Reagent. Additionally, use the PureLink HiPure plasmid prep kit, not the silica-based miniprep kit for bacmid prep.

Find additional tips, troubleshooting help, and resources within our Transfection Support Center.

I accidentally left my lipid reagent at room temperature. Can I still use it?

Yes, all of our lipid transfection reagents are stable at room temperature for months.

Find additional tips, troubleshooting help, and resources within our Lipid-Based Transfection Support Center.

What is the difference between reverse transfection and forward transfection? What should I use?

In forward transfection, cells are seeded to appropriate confluence or cell density in wells or dishes, and the lipid-DNA complexes are added the next day. In reverse transfection, the transfection complexes are prepared inside the wells, after which cells and medium are added. Reverse transfection is faster to perform than forward transfection, and is the method of choice for high-throughput transfection. For non-high-throughput transfections, generally forward transfections have better efficiency for most cell types.

Find additional tips, troubleshooting help, and resources within our Transfection Support Center.

Is there a place where I can find references from other researchers who have used your transfection reagents?

Visit the product page for each reagent type and you will see a list of references at the bottom of the page. A table that lists specific cell line references is also accessible. We also recommend www.highwire.org as a search engine to find a large selection of up-to-date research articles using our transfection products. Simply include the name of the transfection reagent and your cell line/application of interest in your search criteria.

Find additional tips, troubleshooting help, and resources within our Transfection Support Center.

View all Cellfectin™ II Reagent FAQs