Terminal Deoxynucleotidyl Transferase, recombinant
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Terminal Deoxynucleotidyl Transferase, recombinant
인용 및 참조 문헌 (9)
Invitrogen™

Terminal Deoxynucleotidyl Transferase, recombinant

Terminal Deoxynucleotidyl Transferase, Recombinant (rTdT)는 DNA의 3fi hydroxyl 기 말단에 deoxynucleotides 첨가의 촉매작용을 하는 DNA polymerase입니다. TdT Technical Bulletin을 이용할자세히 알아보기
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카탈로그 번호수량
10533065500 U
105330733 x 500 U
카탈로그 번호 10533065
제품 가격(KRW)
-
수량:
500 U
대량 주문 또는 맞춤형 요청
Terminal Deoxynucleotidyl Transferase, Recombinant (rTdT)는 DNA의 3fi hydroxyl 기 말단에 deoxynucleotides 첨가의 촉매작용을 하는 DNA polymerase입니다. TdT Technical Bulletin을 이용할 수 있습니다.

어플리케이션: 클로닝을 위해 삽입물 및 벡터의 동종중합체 꼬리끌림(Homopolymer tailing). biotin (1,2), 32P- 또는35S-label (3)로 또는 세포자멸사 (TUNEL) (4,5)에서의 onligonucleotide 라벨링.

소스: 송아지 흉선조직 TdT의 baculovirus 클론에서 정제.

성능 및 품질 시험: Endonuclease, 3´ 및 5´ exodeoxyribonuclease, 통합 수준 시험 완료.

Unit 정의: 1unit이 d(pA)50를 프라이머로 사용해 1 nmol dATP를 37°C에서 1시간 내에 산침전가능 물질로 통합.

유해성 경고: 독성; 반응 buffer에 potassium cacodylate 함유. 또한 고독성 화학물질인 염화 코발트 함유.
MSDS를 참조하십시오.

Unit 반응 조건: 0.15 ml의 0.2 M potassium cacodylate (pH 7.2), 10 mM MgO4 C4 H6 , 1 mM 2-mercaptoethanol, 0.5 mg/ml BSA,
100 flM d(pA)50, 1 mM [3H]dATP, enzyme: 37°C에서 1시간.

본 제품은 냉장/냉동제품으로 반송된 제품은 전량 폐기 처리 되오니 주문 전 상세 내용 다시 한번 확인 부탁드립니다.
For Research Use Only. Not for use in diagnostic procedures.
사양
함께 사용가능한 버퍼5X Buffer, Reaction Buffer
제품 유형TdT
수량500 U
배송 조건Approved for shipment on Wet or Dry Ice
효소Terminal Deoxynucleotidyl Transferase
Unit SizeEach
구성 및 보관
Terminal Deoxynucleotidyl Transferase, Recombinant (rTdT) is supplied with vial of 5X buffer [500 mM potassium cacodylate (pH 7.2), 10 mM CoCl2 , 1 mM DTT]. Store at -20°C.

자주 묻는 질문(FAQ)

Is the Terminal Deoxynucleotidyl Transferase (TdT) within the Pierce Biotin 3' End DNA Labeling Kit (Cat. No. 89818) available separately?

Yes, but not the specific one from that same kit. We have the following comparable Terminal Deoxynucleotidyl Transferases (Cat. No. 10533065, 10533073 or EP0161, EP0162).

Find additional tips, troubleshooting help, and resources within our Nucleic Acid Gel Electrophoresis and Blotting Support Center.

인용 및 참조 문헌 (9)

인용 및 참조 문헌
Abstract
Interaction of human nuclear topoisomerase I with guanosine quartet-forming and guanosine-rich single-stranded DNA and RNA oligonucleotides.
Authors: Marchand Christophe; Pourquier Philippe; Laco Gary S; Jing Naijie; Pommier Yves;
Journal:J Biol Chem
PubMed ID:11756434
'Human nuclear DNA topoisomerase I (top1) plays a crucial role in DNA replication, transcription, and chromosome condensation. In this study, we show that intra- and intermolecular guanosine quartets (G-quartets) can inhibit top1-mediated DNA cleavage at a high affinity site. Top1-mediated DNA cleavage was also inhibited by a 16-mer single-stranded oligodeoxynucleotide ... More
The novel WD-repeat protein MORG1 acts as a molecular scaffold for HIF prolyl-hydroxylase 3 (PHD3).
Authors:Hopfer U, Hopfer H, Jablonski K, Stahl RA, Wolf G,
Journal:J Biol Chem
PubMed ID:16407229
'Hypoxia-inducible factor-1 (HIF-1), a transcriptional complex composed of an oxygen-sensitive alpha- and a beta-subunit, plays a pivotal role in cellular adaptation to low oxygen availability. Under normoxia, the alpha-subunit of HIF-1 is hydroxylated by a family of prolyl hydroxylases (PHDs) and consequently targeted for proteasomal degradation. Three different prolyl hydroxylases ... More
Coordinated transcription of key pathways in the mouse by the circadian clock.
Authors: Panda Satchidananda; Antoch Marina P; Miller Brooke H; Su Andrew I; Schook Andrew B; Straume Marty; Schultz Peter G; Kay Steve A; Takahashi Joseph S; Hogenesch John B;
Journal:Cell
PubMed ID:12015981
'In mammals, circadian control of physiology and behavior is driven by a master pacemaker located in the suprachiasmatic nuclei (SCN) of the hypothalamus. We have used gene expression profiling to identify cycling transcripts in the SCN and in the liver. Our analysis revealed approximately 650 cycling transcripts and showed that ... More
DEMETER, a DNA glycosylase domain protein, is required for endosperm gene imprinting and seed viability in arabidopsis.
Authors: Choi Yeonhee; Gehring Mary; Johnson Lianna; Hannon Mike; Harada John J; Goldberg Robert B; Jacobsen Steven E; Fischer Robert L;
Journal:Cell
PubMed ID:12150995
'We isolated mutations in Arabidopsis to understand how the female gametophyte controls embryo and endosperm development. For the DEMETER (DME) gene, seed viability depends only on the maternal allele. DME encodes a large protein with DNA glycosylase and nuclear localization domains. DME is expressed primarily in the central cell of ... More
The orphan nuclear receptor REV-ERBalpha controls circadian transcription within the positive limb of the mammalian circadian oscillator.
Authors: Preitner Nicolas; Damiola Francesca; Lopez-Molina Luis; Zakany Joszef; Duboule Denis; Albrecht Urs; Schibler Ueli;
Journal:Cell
PubMed ID:12150932
Mammalian circadian rhythms are generated by a feedback loop in which BMAL1 and CLOCK, players of the positive limb, activate transcription of the cryptochrome and period genes, components of the negative limb. Bmal1 and Per transcription cycles display nearly opposite phases and are thus governed by different mechanisms. Here, we ... More
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