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인용 및 참조 문헌 (2)
Gibco™
DMEM/F-12, GlutaMAX™ supplement
DMEM/F-12 (Dulbecco's Modified Eagle Medium/Nutrient Mixture F-12) is a widely used basal medium for supporting the growth of many different자세히 알아보기
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보기 방식 변경
| 카탈로그 번호 | 수량 |
|---|---|
| 10565042 | 10 x 500 mL |
| 10565018 | 500 mL |
| 31331028 31331-028으로도 사용됨 | 500 mL |
카탈로그 번호 10565042
제품 가격(KRW)
640,000
Online offer
Ends: 31-Dec-2025
673,000할인액 33,000 (5%)
Each
수량:
10 x 500 mL
제품 가격(KRW)
640,000
Online offer
Ends: 31-Dec-2025
673,000할인액 33,000 (5%)
Each
DMEM/F-12 (Dulbecco's Modified Eagle Medium/Nutrient Mixture F-12) is a widely used basal medium for supporting the growth of many different mammalian cells. Cells successfully cultured in DMEM/F-12 include MDCK, glial cells, fibroblasts, human endothelial cells, and rat fibroblasts. We offer a variety of DMEM/F-12 modifications for a range of cell culture applications. Find the right formulation using the media selector tool.
This DMEM / F-12 is modified as follows:
The complete formulation is available.
Using DMEM/F-12
DMEM/F-12 is a 1:1 mixture of DMEM and Ham's F-12. This formulation combines DMEM's high concentrations of glucose, amino acids, and vitamins with F-12's wide variety of components. DMEM/F-12 with GlutaMAX™ supplement minimizes toxic ammonia build-up and improves cell viability and growth in an easy-to-use format. DMEM/F-12 contains no proteins, lipids, or growth factors. Therefore, DMEM/F-12 may require supplementation, commonly with 10% Fetal Bovine Serum (FBS). DMEM/F-12 uses a sodium bicarbonate buffer system and therefore requires a 5–10% CO2 environment to maintain physiological pH.
cGMP manufacturing and quality system
DMEM/F-12 is manufactured at a cGMP-compliant facility located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards. For supply chain continuity, we offer an identical DMEM/F-12 product made in our Scotland facility (31331-028). This facility is also registered with the FDA as a medical device manufacturer and certified to the ISO 13485 standard.
This product is not used for in vitro diagnostic purpose in some countries.
This DMEM / F-12 is modified as follows:
| With | Without |
| • GlutaMAX™ | • HEPES |
| • Phenol Red |
The complete formulation is available.
Using DMEM/F-12
DMEM/F-12 is a 1:1 mixture of DMEM and Ham's F-12. This formulation combines DMEM's high concentrations of glucose, amino acids, and vitamins with F-12's wide variety of components. DMEM/F-12 with GlutaMAX™ supplement minimizes toxic ammonia build-up and improves cell viability and growth in an easy-to-use format. DMEM/F-12 contains no proteins, lipids, or growth factors. Therefore, DMEM/F-12 may require supplementation, commonly with 10% Fetal Bovine Serum (FBS). DMEM/F-12 uses a sodium bicarbonate buffer system and therefore requires a 5–10% CO2 environment to maintain physiological pH.
cGMP manufacturing and quality system
DMEM/F-12 is manufactured at a cGMP-compliant facility located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards. For supply chain continuity, we offer an identical DMEM/F-12 product made in our Scotland facility (31331-028). This facility is also registered with the FDA as a medical device manufacturer and certified to the ISO 13485 standard.
This product is not used for in vitro diagnostic purpose in some countries.
For Research Use or Further Manufacturing. Not for diagnostic use or direct administration into humans or animals.
사양
세포주MDCK, glial cells, fibroblasts, human endothelial cells, and rat fibroblasts
농도1 X
제조 품질cGMP-compliant under the ISO 13485 standard
제품라인Gibco, GlutaMAX
제품 유형DMEM (Dulbecco's Modified Eagle Medium)/F-12
수량10 x 500 mL
유통 기한12 Months From Date of Manufacture
배송 조건Room Temperature
분류Animal Origin-free
형태Liquid
Serum LevelStandard Serum Supplementation
멸균Sterile-filtered
Sterilization MethodSterile-filtered
첨가제 포함High Glucose, GlutaMAX, Phenol Red, Sodium Pyruvate
첨가제 없음No HEPES
Unit SizeEach
구성 및 보관
Storage conditions: 2°C to 8°C (protect from light)
Shipping conditions: Ambient
Shelf life: 12 months from date of manufacture
Shipping conditions: Ambient
Shelf life: 12 months from date of manufacture
자주 묻는 질문(FAQ)
Do you offer DMEM/F12 medium without folic acid?
How long can I keep my media after supplementing with serum?
My medium was shipped at room temperature but it is supposed to be stored refrigerated. Is it okay?
Will depletion, absence, or breakdown of essential growth-promoting components such as glutamine or growth factors reduce the growth rate of my culture?
Will the media formulations containing GlutaMAX supplement change with respect to L-glutamine content?
인용 및 참조 문헌 (2)
인용 및 참조 문헌
Abstract
Fabrication of synthetic polymer coatings and their use in feeder-free culture of human embryonic stem cells.
Journal:Nat Protoc
PubMed ID:21720316
The culture of human embryonic stem (hES) cells in defined and xenogeneic-free conditions will contribute substantially to future biotechnological and medical applications. To achieve this goal, we developed the first fully defined synthetic polymer coating poly[2-(methacryloyloxy)ethyl dimethyl-(3-sulfopropyl)ammonium hydroxide] (PMEDSAH) that sustains long-term growth of hES cells in different culture media.
Derivation of high-purity oligodendroglial progenitors.
Journal:Methods Mol Biol
PubMed ID:19378196
Oligodendrocytes are a type of glial cells that play a critical role in supporting the central nervous system (CNS), in particular insulating axons within the CNS by wrapping them with a myelin sheath, thereby enabling saltatory conduction. They are lost, and myelin damaged - demyelination - in a wide variety