Hygromycin B (50 mg/mL)
Hygromycin B (50 mg/mL)
인용 및 참조 문헌 (38)
Gibco™

Hygromycin B (50 mg/mL)

Hygromycin B는 박테리아 Streptomyces hydroscopicus에서 정제한 수용성 항생제입니다. Hygromycin B는 박테리아 리소좀 80S에 결합하여 단백질 합성을 억제합니다. Gibco™ Hygromycin B는자세히 알아보기
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카탈로그 번호수량
1068701020 mL
카탈로그 번호 10687010
제품 가격(KRW)
406,000
Online offer
Ends: 31-Mar-2026
451,000
할인액 45,000 (10%)
Each
카트에 추가하기
수량:
20 mL
제품 가격(KRW)
406,000
Online offer
Ends: 31-Mar-2026
451,000
할인액 45,000 (10%)
Each
카트에 추가하기
Hygromycin B는 박테리아 Streptomyces hydroscopicus에서 정제한 수용성 항생제입니다. Hygromycin B는 박테리아 리소좀 80S에 결합하여 단백질 합성을 억제합니다. Gibco™ Hygromycin B는 100 - 1000 μg ⁄ ml 농도에서 박테리아 선택적인 항생제로 사용됩니다. 이 제품은 50 mg ⁄ ml 용액으로 공급됩니다. Hygromycin B 내성은 E. coli hygromycin 내성 유전자(hyg 또는 hph) 때문에 생깁니다. Hygromycin B는 Geneticin™, Blasticidin S, or Zeocin™와 다른 작용기전을 사용하기 때문에 다른 선택 제제와 병용하여 두 가지 선택 실험을 할 수 있습니다(선택 항생제 참조).

용도
이 제품은 연구용으로만 사용가능합니다. 치료 또는 진단 목적으로 동물이나 인간에 사용할 수 없습니다.
For Research Use Only. Not for use in diagnostic procedures.
사양
농도50 mg/mL
배양 유형Mammalian Cell Culture, Insect Cell Culture
용도(애플리케이션)Eukaryotic Selection⁄Stable Cell Line Generation
수량20 mL
배송 조건Room Temperature
형태Liquid
제품 유형Antibiotic
멸균Sterile
Unit SizeEach
구성 및 보관
Storage conditions: 2 to 8°C
Shipping conditions: Ambient

자주 묻는 질문(FAQ)

Which of your antibiotics (Geneticin, Zeocin, Hygromycin B, Blasticidin, and Puromycin) can be used together for stable selection in mammalian cells?

All of our antibiotics (Geneticin, Zeocin, Hygromycin B, Blasticidin, and Puromycin) can be used together for making multiple stable cell lines. However, kill curves will need to be performed for each combination of antibiotics since sensitivity to a given antibiotic tends to increase when combined with other antibiotics.

What are the recommended concentrations of antibiotics to use for selection in prokaryotes and eukaryotes?

For best results, optimal concentrations for selection should be determined empirically in each unique experiment through dose response curves. However, to get a general idea of concentrations that have worked for individual cell types, please click on the following url: http://www.thermofisher.com/us/en/home/life-science/cell-culture/transfection/selection.html or type in “Selection Antibiotics” into our main search on www.thermofisher.com.

What is the range of specific activity of Hygromycin B?

The specific activity range of Hygromycin B is from 386-482 micrograms/mg.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

What solvent is used to prepare your Hygromycin B solution? How should it be stored?

Hygromycin B is supplied as a 50 mg/mL solution in phosphate buffered saline. It is manufactured aseptically and sterile filtered, and is suitable for use in tissue culture applications. We recommend storing it at 2-8 degrees C.

What is the mode of action on the following antibiotics: Blasticidin, Geneticin (G418), Hygromycin, and Zeocin?

Blasticidin: Nucleoside Inhibits protein synthesis in prokaryotic and eukaryotic cells by interfering with peptidyl transfer reaction of protein synthesis, causing early termination of translation.

Geneticin (G418): Aminoglycoside Blocks protein synthesis in mammalian cells by interfering with ribosomal function.

Hygromycin: Aminocyclitol Inhibits protein synthesis by disrupting translocation and promoting mistranslation.

Zeocin: Intercalates with DNA and cleaves it.

View all Hygromycin B (50 mg/mL) FAQs

인용 및 참조 문헌 (38)

인용 및 참조 문헌
Abstract
WAF1, a potential mediator of p53 tumor suppression.
Authors:el-Deiry WS, Tokino T, Velculescu VE, Levy DB, Parsons R, Trent JM, Lin D, Mercer WE, Kinzler KW, Vogelstein B
Journal:Cell
PubMed ID:8242752
The ability of p53 to activate transcription from specific sequences suggests that genes induced by p53 may mediate its biological role as a tumor suppressor. Using a subtractive hybridization approach, we identified a gene, named WAF1, whose induction was associated with wild- type but not mutant p53 gene expression in ... More
CC chemokine receptor 5-mediated signaling and HIV-1 Co-receptor activity share common structural determinants. Critical residues in the third extracellular loop support HIV-1 fusion.
Authors:Alkhatib G, Ahuja SS, Light D, Mummidi S, Berger EA, Ahuja SK
Journal:J Biol Chem
PubMed ID:9242636
'There is a close correspondence between the ability of RANTES and macrophage inflammatory proteins 1alpha and 1beta to activate CC chemokine receptor 5 (CCR5) and the ability to inhibit CCR5-dependent membrane fusion mediated by the envelope glycoprotein of human immunodeficiency virus (HIV), type 1. This finding suggests that some of ... More
Dimerization of a selectin and its ligand stabilizes cell rolling and enhances tether strength in shear flow.
Authors: Ramachandran V; Yago T; Epperson T K; Kobzdej M M; Nollert M U; Cummings R D; Zhu C; McEver R P;
Journal:Proc Natl Acad Sci U S A
PubMed ID:11526223
'Selectins mediate rolling of leukocytes by rapid formation and dissociation of selectin-ligand bonds, which are assumed to require high mechanical strength to prevent premature dissociation by the forces applied in shear flow. This assumption is based largely on the observation that increasing wall shear stress increases only modestly the dissociation ... More
Reconstitution of stretch-activated cation channels by expression of the alpha-subunit of the epithelial sodium channel cloned from osteoblasts [published erratum appears in Proc Natl Acad Sci U S A 1997 Apr 15;94(8):4233]
Authors:Kizer N, Guo XL, Hruska K
Journal:Proc Natl Acad Sci U S A
PubMed ID:9023374
'Osteoblasts respond to repetitive strain by activating stretch- activated, nonselective cation channels (SA-CAT) and increasing matrix protein production. SA-CAT channels are thought to be responsible for mechano-transduction in osteoblasts, although the molecular identity of the SA-CAT channel has previously been unknown. We have demonstrated that both the UMR-106 osteoblast-like cell ... More
Genetic analysis of the herpes simplex virus type 1 UL20 protein domains involved in cytoplasmic virion envelopment and virus-induced cell fusion.
Authors:Melancon JM, Foster TP, Kousoulas KG,
Journal:J Virol
PubMed ID:15220406
'The herpes simplex virus type 1 UL20 protein (UL20p) is an important determinant for cytoplasmic virion morphogenesis and virus-induced cell fusion. To delineate the functional domains of the UL20 protein, we generated a panel of single and multiple (cluster) alanine substitutions as well as UL20p carboxyl-terminal truncations. The UL20 mutant ... More
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