Invitrogen 1 Kb Plus DNA Ladder is designed for sizing and approximate quantification of double-stranded DNA in the range of자세히 알아보기
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카탈로그 번호 10787026
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수량:
2000 Applications
대량 주문 또는 맞춤형 요청
제품 가격(KRW)
865,000
온라인 행사
Ends: 31-Mar-2026
961,000
할인액 96,000 (10%)
Each
카트에 추가하기
Invitrogen 1 Kb Plus DNA Ladder is designed for sizing and approximate quantification of double-stranded DNA in the range of 100 bp to 15,000 bp. 1 Kb Plus DNA Ladder consists of 18 individual chromatography-purified DNA fragments and has a reference band at 1,500 bp for easy orientation.
1 Kb DNA Ladder is ideal for separation on 0.8–1% agarose gels.
Highlights of 1 Kb Plus DNA Ladder: • Sharp, clear bands—chromatography purified fragments for consistent and reliable results • Convenient—provided with 10X BlueJuice Gel Loading Buffer for tracking of sample DNA migration • Precise—an exact amount of DNA in each band
Product use The double-stranded DNA ladder can be visualized on 0.8–1% agarose gels after ethidium bromide or SYBR Safe staining. The ladder is designed with a uniform intensity of DNA bands for a clear view of each band. An exact amount of DNA in each band allows approximate quantification of DNA samples.
This ladder can be radiolabeled with T4 polynucleotide kinase or T4 DNA polymerase.
For Research Use Only. Not for use in diagnostic procedures.
사양
농도0.5 μg/μL
젤 호환성Agarose gel
그린 기능Sustainable packaging
키트 구성품4 x 500 μL DNA Ladder, 4 x 1 mL Sample Loading Buffer, 2000 Applications
반응 수2000 Applications
제품 유형DNA Ladder
수량2000 Applications
로드 준비No
샘플 로딩 부피1 mL
배송 조건Approved for shipment at Room Temperature or on Dry Ice
기술Individual chromatography-purified DNA fragments
부피(미터법)500 μL
젤 유형Agarose
크기 범위100 to 15000 bp
Unit SizeEach
구성 및 보관
• 4 x 500 µL 1 Kb Plus DNA Ladder • 4 x 1 mL 10X BlueJuice Gel Loading Buffer
Store at -20°C.
자주 묻는 질문(FAQ)
Can I know the sequences of Invitrogen DNA ladders?
Sequences of Invitrogen DNA and RNA ladders are proprietary.
Are Invitrogen DNA ladders composed of linear or circular/supercoiled DNA?
Invitrogen DNA ladders contain linear dsDNA fragments.
Are Invitrogen DNA ladders composed of single-stranded or double-stranded DNA fragments?
Invitrogen DNA ladders are composed of double-stranded DNA fragments only.
Why are the DNA bands from my molecular weight ladder smearing?
Here are a few reasons why you might see smearing of the bands:
- The DNA was degraded. Avoid nuclease contamination of DNA standards.
- Too much DNA was loaded on the gel. Decrease the amount of DNA in the gel.
- The DNA was contaminated by protein. Remove proteins by phenol extraction before electrophoresis.
- For small DNA, the bands may have diffused during staining. Add the ethidium bromide before electrophoresis.
- For radiolabeled DNA, labeling was performed by nick translation. Label the DNA by replacement synthesis with T4 DNA polymerase or label the 5' end with T4 polynucleotide kinase.
- Improper electrophoresis conditions were used. Do not allow voltage to exceed ~20 V/cm. Maintain a temperature <30°C during electrophoresis. Check that the electrophoresis buffer used has sufficient buffering capacity.
- The DNA contained too much salt. Remove excess salt by ethanol precipitation before electrophoresis.
I'm seeing anomalous migration of my DNA ladder. What happened?
This can happen if the marker was heated. Please ensure that the ladders are not heated before use.
Bisindolylmaleimide VIII enhances DR5-mediated apoptosis through the MKK4/JNK/p38 kinase and the mitochondrial pathways.
Authors: Ohtsuka Toshiaki; Zhou Tong;
Journal:J Biol Chem
PubMed ID:12034736
Bisindolylmaleimide VIII (Bis VIII) has been previously shown to enhance Fas-mediated apoptosis through a protein kinase C-independent mechanism. In the present study, we examined the effect of Bis VIII on apoptosis induced by DR5 (TRAIL-R2), using an agonistic anti-human DR5 monoclonal antibody, TRA-8. Our results demonstrated that Bis VIII was ... More