Platinum™ Taq DNA Polymerase
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Platinum&trade; <i>Taq</i> DNA Polymerase
인용 및 참조 문헌 (2)
Invitrogen™

Platinum™ Taq DNA Polymerase

Invitrogen Platinum Taq DNA Polymerase is a convenient and reliable 'hot start' thermostable DNA polymerase for PCR that provides enhanced specificity over that of Taq DNA Polymerase.
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카탈로그 번호반응 수
10966026300 Reactions
10966018120 Reactions
10966034600 Reactions
109660835000 Reactions
카탈로그 번호 10966026
제품 가격(KRW)
436,000
キャンペーン価格
Ends: 31-Dec-2025
484,000
할인액 48,000 (10%)
Each
카트에 추가하기
반응 수:
300 Reactions
대량 주문 또는 맞춤형 요청
제품 가격(KRW)
436,000
キャンペーン価格
Ends: 31-Dec-2025
484,000
할인액 48,000 (10%)
Each
카트에 추가하기
Invitrogen Platinum Taq DNA Polymerase is a convenient and reliable hot-start thermostable DNA polymerase for PCR that provides enhanced specificity over that of Taq DNA Polymerase. The hot start property of the enzyme is conferred by thermolabile monoclonal antibodies that render Taq DNA polymerase inactive until the initial PCR denaturation step, thus preventing the extention of nonspecifically annealed primers and improving product yield.

The hot-start property allows for convenient reaction assembly at room temperature. Just as with Taq DNA Polymerase, Platinum Taq DNA Polymerase has a non-template dependent terminal transferase activity that adds a 3' deoxyadenosine to product ends and has a 5' to 3' exonuclease activity. PCR products generated with Platinum Taq DNA Polymerase may be used in the same downstream applications without protocol modifications.

Features

  • Convenient reaction assembly at room temperature
  • 5' to 3' exonuclease activity
  • Lacks 3' to 5' exonuclease activity

Applications

  • Amplification of DNA from complex genomic, viral, and plasmid templates
  • Hot-start PCR
  • RT-PCR

Notes

  • For superior PCR performance, the next-generation enzyme Platinum II Taq Hot-start DNA Polymerase is recommended.
  • Platinum II Taq Hot-Start DNA Polymerase is designed for universal primer annealing and fast, easy PCR with its unique combination of innovative buffer, high-performance engineered Taq DNA polymerase, and superior hot-start technology.
For Research Use Only. Not for use in diagnostic procedures.
사양
엑소뉴클레아제 활성5' - 3'
Fidelity (Taq 대비)1X
형식Tube
핫 스타트Built-In Hot Start
반응 수300 Reactions
오버행3'-A
중합효소Platinum Taq DNA Polymerase
제품 유형DNA Polymerase
수량300 reactions
반응 형식Separate Components
배송 조건Wet or Dry Ice
크기(최종 제품)5 kb or less
시작 물질DNA
용도(애플리케이션)Hot-start PCR
GC-Rich PCR PerformanceLow
반응 속도Standard
Unit SizeEach
구성 및 보관
• Platinum Taq DNA Polymerase, 60 μL
• 10X PCR Buffer (without magnesium), 2 x 1.25 mL
• 50 mM MgCl2, 1 mL
• KB Extender, 1.25 mL

Store at -10°C to -30°C in a non-frost-free freezer.

자주 묻는 질문(FAQ)

Can I purchase just the 10X PCR buffer that comes with Platinum Taq?

The 10X PCR buffer for Platinum Taq is not available as a stand-alone item. It is only supplied as part of the enzyme kit.

My oligonucleotide does not appear to be the right length when I checked by gel electrophoresis. Why is this?

Oligos should be run on a polyacrylamide gel containing 7 M urea and loaded with a 50% formamide solution to avoid compressions and secondary structures. Oligos of the same length and different compositions can electrophorese differently. dC's migrate fastest, followed by dA's, dT's, and then dG's. Oligos containing N's tend to run as a blurry band and generally have a problem with secondary structure.

The primers I am using worked for PCR initially, but over time, have stopped working. What happened?

Primers should be aliquoted for single use before PCR set-up. Heat just the aliquoted primers to 94 degrees for 1 min. Quick chill the primer on ice before adding to the PCR reaction. Some primers may anneal to themselves or curl up on themselves.

I don't see a pellet in my oligo tube order. Should I ask for a replacement?

The drying method dries the primer in a thin layer along the sidewalls of the tube instead of the bottom, therefore a pellet is not always visible and should still be ready to use.

There is a ball-shaped pellet at the bottom of my oligo tube. What is this and can I still use my oligo?

If the oligo was overheated, it will appear as a “ball”-shaped pellet attached to the bottom of the tube. This should not affect the quality of the oligo, and the oligo should be readily soluble in water.

View all Platinum™ Taq DNA Polymerase FAQs

인용 및 참조 문헌 (2)

인용 및 참조 문헌
Abstract
Dynamin 2 mutations associated with human diseases impair clathrin-mediated receptor endocytosis.
Authors:Bitoun M, Durieux AC, Prudhon B, Bevilacqua JA, Herledan A, Sakanyan V, Urtizberea A, Cartier L, Romero NB, Guicheney P,
Journal:Hum Mutat
PubMed ID:19623537
Dynamin 2 (DNM2) is a large GTPase involved in the release of nascent vesicles during endocytosis and intracellular membrane trafficking. Distinct DNM2 mutations, affecting the middle domain (MD) and the Pleckstrin homology domain (PH), have been identified in autosomal dominant centronuclear myopathy (CNM) and in the intermediate and axonal forms ... More
Utility and accuracy of template-directed dye-terminator incorporation with fluorescence-polarization detection for genotyping single nucleotide polymorphisms.
Authors: Akula N; Chen Y S; Hennessy K; Schulze T G; Singh G; McMahon F J;
Journal:Biotechniques
PubMed ID:12019780
There are little independent data available about how well single nucleotide polymorphism (SNP) genotyping technologies perform in the typical molecular genetics laboratory. We evaluated the utility and accuracy of a widely used technology, template-directed dye-terminator incorporation with fluorescence-polarization detection (FP-TDI), in a sample of 177 SNPs selected solely on the ... More