MEM
MEM
인용 및 참조 문헌 (8)
Gibco™

MEM

MEM (Minimum Essential Medium)은 가장 널리 사용되는 세포 배양 배지 중 하나입니다. MEM은 HeLa, BHK-21, 293, HEP-2, HT-1080, MCF-7, 섬유아세포,일차자세히 알아보기
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카탈로그 번호수량
11095080500 mL
1109509810 x 500 mL
110950721000 mL
110951146 x 1000 mL
카탈로그 번호 11095080
제품 가격(KRW)
52,000
Offre exceptionnelle en ligne
Ends: 31-Dec-2025
54,000
할인액 2,000 (4%)
Each
카트에 추가하기
수량:
500 mL
Customize this product
제품 가격(KRW)
52,000
Offre exceptionnelle en ligne
Ends: 31-Dec-2025
54,000
할인액 2,000 (4%)
Each
카트에 추가하기
MEM (Minimum Essential Medium)은 가장 널리 사용되는 세포 배양 배지 중 하나입니다. MEM은 HeLa, BHK-21, 293, HEP-2, HT-1080, MCF-7, 섬유아세포,일차 쥐 별아교세포 등 다양한 suspension 및 adherent 포유류 세포에 사용할 수 있습니다. 당사는 다양한 세포 배양 응용 분야에 맞는 여러 가지 MEM modification을 제공합니다. 배지 선택 도구를 사용하여 적절한 조성을 간편하게 찾을 수 있습니다.

이 MEM은 다음과 같이 조성이 변경되었습니다.
포함 제외 성분
• L-글루타민 • HEPES
• 페놀 레드

모든 조성을 이용할 수 있습니다.

MEM의 사용
MEM은 이전의 Basal Medium Eagle(BME)의 제형에 기초하여 Harry Eagle에 의해 개발되었습니다. Glasgow’s MEM, MEM α, DMEM 및 Temin’s Modification을 비롯한 MEM의 기타 많은 변경 제품이 뒤따랐습니다. MEM은 CO2 인큐베이터에서 사용하기 위해 Earle’s salts와 함께 사용하거나 Hanks' salts와 함께 사용하여 CO2 없이 사용할 수 있습니다. 이 제품은 Earle’s salts를 사용하여 제조되었습니다. MEM에는 단백질, 지질, 성장 인자가 들어있지 않습니다. 따라서 MEM을 사용하려면 일반적으로 10% Fetal Bovine Serum(FBS)을 보충해야 합니다. MEM은 중탄산나트륨(sodium bicarbonate) 버퍼 시스템(2.2 g/L)을 이용하며, 이로 인해 생리학적인 pH를 유지하기 위해 5–10% CO2 환경이 요구됩니다.

cGMP 제조 및 품질 시스템
MEM은 뉴욕주의 Grand Island에 위치한 cGMP 준수 시설에서 제조됩니다. 이 시설은 의료기기 제조원으로 FDA에 등록되어 있으며 ISO 13485 표준 인증을 받은 기관입니다. 공급망 유지를 위해 당사는 당사의 스코틀랜드 시설에서도 동일한 MEM 제품을 제공합니다(31095-029). 이 시설은 의료기기 제조원으로 FDA에 등록되어 있으며 ISO 13485 표준 인증을 받은 기관입니다.
For Research Use or Further Manufacturing. Not for diagnostic use or direct administration into humans or animals.
사양
세포주HeLa, BHK-21, 293, HEP-2, HT-1080, MCF-7 및 섬유아세포
세포 유형일차 쥐 별아교세포
농도1X
제조 품질cGMP-compliant under the ISO 13485 standard
제품라인Gibco
제품 유형MEM(Minimum Essential Medium)
수량500 mL
유통 기한제조일로부터 12개월
배송 조건실온
분류동물 원료 성분 비함유
형태액체
Serum LevelStandard Serum Supplementation
멸균멸균 여과
Sterilization MethodSterile-filtered
첨가제 포함L-글루타민, 페놀 레드, Glutamine, Phenol Red
첨가제 없음HEPES, No Sodium Pyruvate
Unit SizeEach
구성 및 보관
보관 조건: 2°C ∼ 8°C(차광 보관)
배송 조건: 상온
유통 기한: 제조일로부터 12개월

자주 묻는 질문(FAQ)

Where can I find the osmolality for MEM Medium?

The osmolality is listed in the COA for the particular lot number of the medium.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

What is the shelf life of the DMEM, high glucose, pyruvate medium once the bottle is opened and the medium is supplemented?

We do not provide stability data for the product once it is opened as it would depend on the usage and storage conditions.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Is it necessary to store DMEM, high glucose, pyruvate medium in the dark?

Yes, the medium should be stored in the dark because there are some components in the medium such as HEPES, Tryptophan, and Riboflavin, etc. that are sensitive to light.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

How long can I keep my media after supplementing with serum?

Generally speaking, media can be used for up to three weeks after supplementation with serum. There are no formal studies to support this, but it is the rule of thumb used by our scientists.

Find additional tips, troubleshooting help, and resources within our Mammalian Cell Culture Basics Support Center.

My medium was shipped at room temperature but it is supposed to be stored refrigerated. Is it okay?

We routinely ship media that require long-term storage in the refrigerator at room temperature. We have done studies on representative media formulations to show that media can be at room temperature for up to a week without a problem.

Find additional tips, troubleshooting help, and resources within our Mammalian Cell Culture Basics Support Center.

View all MEM FAQs

인용 및 참조 문헌 (8)

인용 및 참조 문헌
Abstract
Influx of calcium through a redox-sensitive plasma membrane channel in thymocytes causes early necrotic cell death induced by the epipolythiodioxopiperazine toxins.
Authors: Hurne Alanna M; Chai Christina L L; Moerman Katherine; Waring Paul;
Journal:J Biol Chem
PubMed ID:12063251
'Gliotoxin, a member of the epipolythiodioxopiperazine (ETP) class of toxins, induces both apoptotic and necrotic cell death in a concentration-dependent manner. Whereas the specific trigger for apoptotic death caused by these toxins is unclear, the reactive disulfide bond in the ETP toxins is required for biological activity. Thus it is ... More
Antagonism between Ena/VASP Proteins and Actin Filament Capping Regulates Fibroblast Motility.
Authors: Bear James E; Svitkina Tatyana M; Krause Matthias; Schafer Dorothy A; Loureiro Joseph J; Strasser Geraldine A; Maly Ivan V; Chaga Oleg Y; Cooper John A; Borisy Gary G; Gertler Frank B;
Journal:Cell
PubMed ID:12086607
'Cell motility requires lamellipodial protrusion, a process driven by actin polymerization. Ena/VASP proteins accumulate in protruding lamellipodia and promote the rapid actin-driven motility of the pathogen Listeria. In contrast, Ena/VASP negatively regulate cell translocation. To resolve this paradox, we analyzed the function of Ena/VASP during lamellipodial protrusion. Ena/VASP-deficient lamellipodia protruded ... More
A serum- and antioxidant-free primary culture model of mouse cortical neurons for pharmacological screen and studies of neurotrophic and neuroprotective agents.
Authors:Yu X, An L,
Journal:Cell Mol Neurobiol
PubMed ID:12363202
'1. Morphologically developmental properties of fetal mouse cortical neurons in the chemically defined serum- and antioxidant-free culture condition were observed. Also, cellular composition in cultures was identified by immunostaining with anti-NSE and anti-GFAP. 2. Various cell densities ranging from 1 x 10(3) to 1 x 10(6) cells/cm2 were prepared to ... More
Acute hippocampal slice preparation and hippocampal slice cultures.
Authors:Lein PJ, Barnhart CD, Pessah IN
Journal:Methods Mol Biol
PubMed ID:21815062
'A major advantage of hippocampal slice preparations is that the cytoarchitecture and synaptic circuits of the hippocampus are largely retained. In neurotoxicology research, organotypic hippocampal slices have mostly been used as acute ex vivo preparations for investigating the effects of neurotoxic chemicals on synaptic function. More recently, hippocampal slice cultures, ... More
The Discodermia calyx toxin calyculin a enhances cyclin D1 phosphorylation and degradation, and arrests cell cycle progression in human breast cancer cells.
Authors:Edelson JR, Brautigan DL
Journal:Toxins (Basel)
PubMed ID:22069692
'Cyclin D1 is a key regulator of the cell cycle that is over expressed in more than half of breast cancer patients. The levels of cyclin D1 are controlled primarily through post-translational mechanisms and phosphorylation of cyclin D1 at T286 induces its proteasomal degradation. To date, no studies have explored ... More
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