Human Endothelial SFM

Human Endothelial Serum Free Medium (SFM) has been developed to support the long-term propagation of human umbilical vein endothelial cells (HUVEC). Complete Human Endothelial SFM has been demonstrated to successfully support primary isolation and subsequent secondary growth of HUVEC for ≤15 passages. HUVEC cultured in complete Human Endothelial SFM exhibit the histotypic 'cobblestone' morphology and retain endothelial specific markers including: expression of Factor VIII-related antigen, UEA-1 lectin binding, uptake of DiI-acetylated LDL, vimentin, and IL-1a-induced ICAM-1 expression. Additionally, HUVEC cultured in Human Endothelial SFM have been shown to maintain cAMP and prostacyclin signal transduction systems. Human Endothelial SFM also supports the growth and retention of physiological markers for human umbilical arterial and dermal microvascular endothelial cells.

Human Endothelial SFM is a basal serum-free growth medium and requires supplementation with bFGF (20 ng/mL), EGF (10 ng/mL), and human plasma fibronectin (10 μg/mL).