ElectroMAX™ DH5α-E Competent Cells

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Invitrogen™

ElectroMAX™ DH5α-E Competent Cells

ElectroMAX DH5α-E Competent Cells are derived from the DH5α strain and are suitable for transformation by electroporation. It is challenging자세히 알아보기

카탈로그 번호	수량
11319019	5 x 100 μL

카탈로그 번호 11319019

제품 가격(KRW)

482,000

온라인 행사

Ends: 31-Dec-2025

566,000

할인액 84,000 (15%)

카트에 추가하기

5 x 100 μL

제품 가격(KRW)

482,000

온라인 행사

Ends: 31-Dec-2025

566,000

할인액 84,000 (15%)

카트에 추가하기

ElectroMAX DH5α-E Competent Cells are derived from the DH5α strain and are suitable for transformation by electroporation. It is challenging to achieve high transformation efficiency by electroporation using the standard DH5α strain (chemical transformation is more efficient) thus additional mutations were introduced to create the DH5α-E strain that is able to reach >1 x 10¹⁰ efficiency by electroporation with non-saturating amounts of DNA. Using this strain, 10 to 50 times greater colony number may be obtained in a single transformation than using the chemically competent DH5α cells. Therefore DH5α-E competent cells are ideal for challenging applications, such as generation of cDNA libraries or in transformations with limited DNA input.

ElectroMAX DH5α-E Competent Cells share several key genetic properties. They carry the hsdR mutation to prevent restriction of foreign DNA and the endA1 mutation that increases the yield of plasmid DNA prepared using miniprep protocols. The lacZΔM15 marker provides α-complementation of the ß-galactosidase gene, allowing blue-white screening on agar plates containing X-gal or Bluo-gal. DH5α-E allows efficient transformation of large plasmids and can also serve as a host for M13mp cloning vectors if a lawn of DH5αF′IQ is provided to allow plaque formation. We do not recommended using this strain for direct cloning of methylated genomic DNA.

ElectroMAX DH5α-E Competent Cell features
• Greatly increased plasmid yield and quality due to endA1 mutation
• High-efficiency transformation with plasmids 30 kb in size
• Blue/white screening of recombinant clones due to lacZΔM15
• Ensured insert stability due to recA1 mutation
• hsdR for efficient transformation of unmethylated DNA from PCR applications

Note: A high-voltage electroporation apparatus is required.

Genotype
F^– φ80lacZΔM15 Δ(lacZYA-argF)U169 recA1 endA1 hsdR17(r_K^–, m_K⁺) gal phoA supE44 λ-thi-1 gyrA96 relA1
Genetic marker descriptions

Find the strain and format that fits your needs
DH strains are available in chemically competent and electrocompetent cell formats.
Explore Multishot format for high throughput applications.
MAX Efficiency DH5αF´IQ Competent Cells are available in chemically competent format.
Explore bacterial growth media formats.

For Research Use Only. Not for use in diagnostic procedures.

항생제 내성 박테리아No

블루/화이트 스크리닝Yes (lacZΔM15)

메틸화 DNA 클로닝No

불안정 DNA 클로닝Not suitable for cloning unstable DNA

에프에피솜 포함No

고처리량 호환성Low

플라스미드 품질 개선Yes (endA1)

플라스미드May be used for plasmids >20 kb

비메틸화 DNA 준비No

제품라인ElectroMAX

제품 유형Electrocompetent Cells

수량5 x 100 μL

재조합 감소Yes (recA1)

배송 조건Dry Ice

T1 Phage - 저항성(tonA)No

형질전환 효율 수준High Efficiency (>1 x 10⁹ cfu/μg)

형식Tube

종E. coli (K12)

Unit SizeEach

구성 및 보관

• ElectroMAX DH5α-E Competent Cells (5 x 100 μL)
Store Competent Cells at –80°C.

• pUC19 DNA (50 μL at 10 pg/μL)
Store pUC19 DNA at –20°C.

• S.O.C. Medium (2 x 6 mL)
Store S.O.C. Medium at 4°C or room temperature.

자주 묻는 질문(FAQ)

How do you recommend that I prepare my DNA for successful electroporation of E. coli?

For best results, DNA used in electroporation must have a very low ionic strength and a high resistance. A high-salt DNA sample may be purified by either ethanol precipitation or dialysis.

The following suggested protocols are for ligation reactions of 20ul. The volumes may be adjusted to suit the amount being prepared.

Purifying DNA by Precipitation: Add 5 to 10 ug of tRNA to a 20ul ligation reaction. Adjust the solution to 2.5 M in ammonium acetate using a 7.5 M ammonium acetate stock solution. Mix well. Add two volumes of 100 % ethanol. Centrifuge at 12,000 x g for 15 min at 4C. Remove the supernatant with a micropipet. Wash the pellet with 60ul of 70% ethanol. Centrifuge at 12,000 x g for 15 min at room temperature. Remove the supernatant with a micropipet. Air dry the pellet. Resuspend the DNA in 0.5X TE buffer [5 mM Tris-HCl, 0.5 mM EDTA (pH 7.5)] to a concentration of 10 ng/ul of DNA. Use 1 ul per transformation of 20 ul of cell suspension.

Purifying DNA by Microdialysis: Float a Millipore filter, type VS 0.025 um, on a pool of 0.5X TE buffer (or 10% glycerol) in a small plastic container. Place 20ul of the DNA solution as a drop on top of the filter. Incubate at room temperature for several hours. Withdraw the DNA drop from the filter and place it in a polypropylene microcentrifuge tube. Use 1ul of this DNA for each electrotransformation reaction.

When should DMSO, formamide, glycerol and other cosolvents be used in PCR?

Cosolvents may be used when there is a failure of amplification, either because the template contains stable hairpin-loops or the region of amplification is GC-rich. Keep in mind that all of these cosolvents have the effect of lowering enzyme activity, which will decrease amplification yield. For more information see P Landre et al (1995). The use of co-solvents to enhance amplification by the polymerase chain reaction. In: PCR Strategies, edited by MA Innis, DH Gelfand, JJ Sninsky. Academic Press, San Diego, CA, pp. 3-16.

Additionally, when amplifying very long PCR fragments (greater than 5 kb) the use of cosolvents is often recommended to help compensate for the increased melting temperature of these fragments.

Find additional tips, troubleshooting help, and resources within our PCR and cDNA Synthesis Support Center.