DMEM/F-12, HEPES
DMEM/F-12, HEPES
인용 및 참조 문헌 (5)
Gibco™

DMEM/F-12, HEPES

DMEM/F-12 (Dulbecco's Modified Eagle Medium/Nutrient Mixture F-12)는 많은 다양한 포유류 세포의 성장을 지원하기 위해 널리 사용되는 기본 배지입니다. DMEM/F-12에서 배양이자세히 알아보기
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카탈로그 번호수량
11330032500mL
1133005710 x 500mL
113300995L
1133010710L
카탈로그 번호 11330032
제품 가격(KRW)
74,000
온라인 행사
Ends: 31-Dec-2025
77,000
할인액 3,000 (4%)
Each
카트에 추가하기
수량:
500mL
Customize this product
제품 가격(KRW)
74,000
온라인 행사
Ends: 31-Dec-2025
77,000
할인액 3,000 (4%)
Each
카트에 추가하기
DMEM/F-12 (Dulbecco's Modified Eagle Medium/Nutrient Mixture F-12)는 많은 다양한 포유류 세포의 성장을 지원하기 위해 널리 사용되는 기본 배지입니다. DMEM/F-12에서 배양이 가능한 세포는 MDCK, 신경교세포, 섬유아세포, 인간 내피 세포 및 쥐 섬유아세포입니다. 당사는 광범위한 세포 배양 응용 분야에 따라 다양하게 조성이 변경된 DMEM/F-12 배지를 제공합니다. 배지 선택 도구를 사용하여 적절한 조성을 간편하게 찾을 수 있습니다.

이 DMEM/F-12는 다음과 같이 조성이 변경되었습니다.
포함
• L-글루타민
• HEPES
• 페놀 레드

모든 조성을 이용할 수 있습니다.

DMEM/F-12의 사용
DMEM/F-12는 DMEM과 Ham F-12의 1:1 혼합물입니다. 이 조성은 DMEM의 고농도 포도당, 아미노산, 비타민과 F-12의 다양한 성분을 결합한 것입니다. DMEM/F-12에는 단백질, 지질, 성장 인자가 들어있지 않습니다. 따라서 DMEM/F-12를 사용하려면 일반적으로 10% Fetal Bovine Serum(FBS)을 사용해야 할 수도 있습니다. DMEM/F-12는 중탄산나트륨(sodium bicarbonate) 버퍼 시스템을 이용하며, 이로 인해 생리학적인 pH를 유지하기 위해 5–10% CO2 환경이 요구됩니다.

cGMP 제조 및 품질 시스템
DMEM/F-12는 뉴욕주의 Grand Island에 위치한 cGMP 준수 시설에서 제조됩니다. 이 시설은 의료기기 제조원으로 FDA에 등록되어 있으며 ISO 13485 표준 인증을 받은 기관입니다. 공급망 유지를 위해 당사는 당사의 스코틀랜드 시설에서도 동일한 DMEM/F-12 제품을 제공합니다(31330-038). 이 시설은 의료기기 제조원으로 FDA에 등록되어 있으며 ISO 13485 표준 인증을 받은 기관입니다.
For Research Use or Further Manufacturing. Not for diagnostic use or direct administration into humans or animals.
사양
세포주MDCK, 아교세포, 섬유아세포, 인간 내피 세포 및 쥐 섬유아세포
농도1X
용도(애플리케이션)포유류 세포 배양
제조 품질cGMP-compliant under the ISO 13485 standard
제품라인Gibco
제품 유형DMEM(Dulbecco's Modified Eagle Medium)/F-12
수량500mL
유통 기한제조일로부터 12개월
배송 조건실온
분류동물 원료 성분 비함유
형태액체
Serum LevelStandard Serum Supplementation
멸균멸균 여과
Sterilization MethodSterile-filtered
첨가제 포함L-글루타민, HEPES, 페놀 레드, Glutamine, HEPES, Phenol Red, Sodium Pyruvate
Unit SizeEach
구성 및 보관
보관 조건: 2°C ∼ 8°C(차광 보관)
배송 조건: 상온
유통 기한: 제조일로부터 12개월

자주 묻는 질문(FAQ)

The lids of DMEM/F-12, HEPES medium (Cat. No. 31330038, 11330032, 31330095, 11330057, 11330107) have a different color now. Have there been any changes in the composition of the medium?

The color change of the lid has no effect on the sterility or functionality of the product. There has been no change to the end product DMEM/F-12, HEPES medium (Cat. No. 31330038, 11330032, 31330095, 11330057, 11330107); the medium still has the same composition.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Do you offer DMEM/F12 medium without folic acid?

We do not offer DMEM/F12 medium without folic acid as a standard catalog product. However, our Customs group can make it for you as a custom media. If you are interested, please complete this Custom order inquiry form PDF (https://www.thermofisher.com/content/dam/LifeTech/global/applied-sciences/pdfs/Bioproduction/Gibco-Custom-Media-Buffers-Request-Form.pdf) and email it to custommedia@thermofisher.com. Our Customs group will review your requirements and get back to you within a couple of days with pricing, ordering information, and lead time. After that, you have the choice of proceeding with an “official” order or declining.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

What is the shelf life of the DMEM, high glucose, pyruvate medium once the bottle is opened and the medium is supplemented?

We do not provide stability data for the product once it is opened as it would depend on the usage and storage conditions.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Is it necessary to store DMEM, high glucose, pyruvate medium in the dark?

Yes, the medium should be stored in the dark because there are some components in the medium such as HEPES, Tryptophan, and Riboflavin, etc. that are sensitive to light.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

How long can I keep my media after supplementing with serum?

Generally speaking, media can be used for up to three weeks after supplementation with serum. There are no formal studies to support this, but it is the rule of thumb used by our scientists.

Find additional tips, troubleshooting help, and resources within our Mammalian Cell Culture Basics Support Center.

View all DMEM/F-12, HEPES FAQs

인용 및 참조 문헌 (5)

인용 및 참조 문헌
Abstract
Patient-derived glioblastoma organoids as real-time avatars for assessing responses to clinical CAR-T cell therapy
Authors:Meghan Logun, Xin Wang, Yusha Sun, Stephen J Bagley, Nannan Li, Arati Desai, Daniel Y Zhang, MacLean P Nasrallah, Emily Ling-Lin Pai, Bike Su Oner, Gabriela Plesa, Donald Siegel, Zev A Binder, Guo-Li Ming, Hongjun Song, Donald M O'Rourke
Journal:Cell Stem Cell
PubMed ID:39657679
Patient-derived tumor organoids have been leveraged for disease modeling and preclinical studies but rarely applied in real time to aid with interpretation of patient treatment responses in clinics. We recently demonstrated early efficacy signals in a first-in-human, phase 1 study of dual-targeting chimeric antigen receptor (CAR)-T cells (EGFR-IL13Rα2 CAR-T cells) ... More
Fabrication of synthetic polymer coatings and their use in feeder-free culture of human embryonic stem cells.
Authors:Nandivada H, Villa-Diaz LG, O'Shea KS, Smith GD, Krebsbach PH, Lahann J,
Journal:Nat Protoc
PubMed ID:21720316
The culture of human embryonic stem (hES) cells in defined and xenogeneic-free conditions will contribute substantially to future biotechnological and medical applications. To achieve this goal, we developed the first fully defined synthetic polymer coating poly[2-(methacryloyloxy)ethyl dimethyl-(3-sulfopropyl)ammonium hydroxide] (PMEDSAH) that sustains long-term growth of hES cells in different culture media. ... More
Insulin and insulin-like growth factor I receptors utilize different G protein signaling components.
Authors:Dalle S, Ricketts W, Imamura T, Vollenweider P, Olefsky JM,
Journal:J Biol Chem
PubMed ID:11278773
We examined the role of heterotrimeric G protein signaling components in insulin and insulin-like growth factor I (IGF-I) action. In HIRcB cells and in 3T3L1 adipocytes, treatment with the Galpha(i) inhibitor (pertussis toxin) or microinjection of the Gbetagamma inhibitor (glutathione S-transferase-betaARK) inhibited IGF-I and lysophosphatidic acid-stimulated mitogenesis but had no ... More
Polycystin-1, the product of the polycystic kidney disease 1 gene, co-localizes with desmosomes in MDCK cells.
Authors:Scheffers MS, van der Bent P, Prins F, Spruit L, Breuning MH, Litvinov SV, de Heer E, Peters DJ,
Journal:Hum Mol Genet
PubMed ID:11063733
Polycystin-1 is a novel protein predicted to be a large membrane-spanning glycoprotein with an extracellular N-terminus and an intracellular C-terminus, harboring several structural motifs. To study the subcellular localization, antibodies raised against various domains of polycystin-1 and against specific adhesion complex proteins were used for two-color immunofluorescence staining. In Madine ... More
alpha-Synuclein interacts with phospholipase D isozymes and inhibits pervanadate-induced phospholipase D activation in human embryonic kidney-293 cells.
Authors: Ahn Bong-Hyun; Rhim Hyangshuk; Kim Shi Yeon; Sung Young-Mo; Lee Mun-Yong; Choi Ju-Youn; Wolozin Benjamin; Chang Jong-Soo; Lee Young Han; Kwon Taeg Kyu; Chung Kwang Chul; Yoon Shin-Hee; Hahn Sang June; Kim Myung-Suk; Jo Yang-Hyeok; Min Do Sik;
Journal:J Biol Chem
PubMed ID:11821392
alpha-Synuclein has been implicated in the pathogenesis of many neurodegenerative diseases, including Parkinson's disease and Alzheimer's disease. Although the function of alpha-synuclein remains largely unknown, recent studies have demonstrated that this protein can interact with phospholipids. To address the role of alpha-synuclein in neurodegenerative disease, we have investigated whether it ... More