PLUS™ Reagent
PLUS™ Reagent
인용 및 참조 문헌 (90)
Invitrogen™

PLUS™ Reagent

PLUS™ Reagent은 Lipofectamine™ 같은 transfection agent와 함께 사용해 adherent cell line의 tranfection을 향상시킵니다. 어떤 고객은 높은 transfection 성능을 얻기 위해자세히 알아보기
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카탈로그 번호수량
115140150.85 mL
카탈로그 번호 11514015
제품 가격(KRW)
433,000
0.85 mL
카트에 추가하기
수량:
0.85 mL
제품 가격(KRW)
433,000
0.85 mL
카트에 추가하기
PLUS™ Reagent은 Lipofectamine™ 같은 transfection agent와 함께 사용해 adherent cell line의 tranfection을 향상시킵니다. 어떤 고객은 높은 transfection 성능을 얻기 위해 DNA 농도 최적화가 덜한 Plus™ Reagent가 필요하다고 합니다. Plus™ Reagent를 추가하면 필요한 DNA와 lipid 양을 lipid 단독 경우보다 절반 정도로 줄일 수 있습니다. PLUS™ Reagent는 transfection이 가능한 세포 유형 array를 확장시키도록 특별히 고안되었습니다(1). Lipofectamine™ Reagent 와 PLUS™ Reagent를 함께 사용했을 때 BHK-21, NIH 3T3 등의 cell line transfection이 개선된다고 입증되었습니다(2).
For Research Use Only. Not for use in diagnostic procedures.
사양
용도(애플리케이션)Transfection
고처리량 호환성Not High-throughput Compatible (Manual)
제품라인PLUS
제품 유형Reagent
수량0.85 mL
혈청 호환 가능Yes
배송 조건Wet Ice
셀 유형Established Cell Lines, Stem Cells, Primary Cells, Hard-to-Transfect Cells
형식6-well Plate, 12-well Plate, 24-well Plate, 48-well Plate, 96-well Plate, Flasks
샘플 종류Plasmid DNA, RNAi Plasmids (shRNA, miR)
Transfection TechniqueLipid-based Transfection
Unit Size0.85 mL
구성 및 보관
Contains one vial (0.85 ml) PLUS™ Reagent. Store at 4°C. Do not freeze.

자주 묻는 질문(FAQ)

I accidentally left my lipid reagent at room temperature. Can I still use it?

Yes, all of our lipid transfection reagents are stable at room temperature for months.

Find additional tips, troubleshooting help, and resources within our Lipid-Based Transfection Support Center.

Do you offer a reagent for the transfection of endothelial cells?

We recommend using the Lipofectamine 3000 Reagent for transfection of endothelial cells.

Find additional tips, troubleshooting help, and resources within our Transfection Support Center.

Can I use the PLUS Reagent with Lipofectamine RNAiMAX?

The PLUS Reagent will not enhance transfection efficiency when used with Lipofectamine RNAiMAX.

Find additional tips, troubleshooting help, and resources within our Transfection Support Center.

Why do I have to dilute my DNA in medium before adding the PLUS Reagent? Can I just add them together?

It is important to dilute the DNA with Opti-MEM I Reduced Serum Medium before adding the PLUS Reagent or the DNA may precipitate.

Find additional tips, troubleshooting help, and resources within our Transfection Support Center.

What is the PLUS Reagent?

PLUS Reagent is a proprietary reagent for pre-complexing DNA. It enhances cationic lipid-mediated transfection of DNA into many cultured eukaryotic cells when used in conjunction with a transfection reagent. Use PLUS Reagent to enhance transfection results with Lipofectamine Reagent, Lipofectamine LTX Reagent, Lipofectin Reagent, Cellfectin Reagent, and Oligofectamine Reagent. Lipofectamine LTX is offered in combination with the PLUS Reagent in Cat. Nos. A12621, 15338030, and 15338100. PLUS Reagent is also offered as a stand-alone product (Cat. No. 11514015). We do not recommend using the PLUS Reagent with Lipofectamine 2000 or Lipofectamine 3000.

Find additional tips, troubleshooting help, and resources within our Transfection Support Center.

View all PLUS™ Reagent FAQs

인용 및 참조 문헌 (90)

인용 및 참조 문헌
Abstract
c-Jun is a JNK-independent coactivator of the PU.1 transcription factor.
Authors:Behre G; Whitmarsh A J; Coghlan M P; Hoang T; Carpenter C L; Zhang D E; Davis R J; Tenen D G;
Journal:J Biol Chem
PubMed ID:9988737
The ETS domain transcription factor PU.1 is necessary for the development of monocytes and regulates, in particular, the expression of the monocyte-specific macrophage colony-stimulating factor (M-CSF) receptor, which is critical for monocytic cell survival, proliferation, and differentiation. The bZIP transcription factor c-Jun, which is part of the AP-1 transcription factor ... More
Receptor-selective effects of endogenous RGS3 and RGS5 to regulate mitogen-activated protein kinase activation in rat vascular smooth muscle cells.
Authors:Wang Qin; Liu Min; Mullah Bashar; Siderovski David P; Neubig Richard R;
Journal:J Biol Chem
PubMed ID:12006602
Regulators of G protein signaling (RGS) proteins compose a highly diverse protein family best known for inhibition of G protein signaling by enhancing GTP hydrolysis by Galpha subunits. Little is known about the function of endogenous RGS proteins. In this study, we used synthetic ribozymes targeted to RGS2, RGS3, RGS5, ... More
A
Authors:Chen Zhenhui; Alcayaga Carmen; Suarez-Isla Benjamin A; O'Rourke Brian; Tomaselli Gordon; Marban Eduardo;
Journal:J Biol Chem
PubMed ID:11973330
The large size (six membrane-spanning repeats in each of four domains) and asymmetric architecture of the voltage-dependent Na+ channel has hindered determination of its structure. With the goal of determining the minimum structure of the Na+ channel permeation pathway, we created two stable cell lines expressing the voltage-dependent rat skeletal ... More
Vesicle-associated membrane protein-2/synaptobrevin binding to synaptotagmin I promotes O-glycosylation of synaptotagmin I.
Authors:Fukuda M.
Journal:J Biol Chem
PubMed ID:12048209
Synaptotagmin I (Syt I), an evolutionarily conserved integral membrane protein of synaptic vesicles, is now known to regulate Ca2+-dependent neurotransmitter release. Syt I protein should undergo several post-translational modifications before maturation and subsequent functioning on synaptic vesicles (e.g. N-glycosylation and fatty acylation in vertebrate Syt I), because the apparent molecular ... More
Extracellular Export of Sphingosine Kinase-1 Enzyme. SPHINGOSINE 1-PHOSPHATE GENERATION AND THE INDUCTION OF ANGIOGENIC VASCULAR MATURATION.
Authors:Ancellin Nicolas; Colmont Chantal; Su Joseph; Li Qin; Mittereder Nanette; Chae Sung-Suk; Stefansson Steingrimur; Liau Gene; Hla Timothy;
Journal:J Biol Chem
PubMed ID:11741921
The enzyme sphingosine kinase (SK) catalyzes the formation of sphingosine 1-phosphate (S1P), a bioactive lipid that acts extracellularly on G protein-coupled receptors of the S1P(1)/EDG-1 subfamily. Although S1P is formed in the cytosol of various cells, S1P release is not understood and is controversial because this lipid mediator is also ... More
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