CMRL Medium, no glutamine
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Gibco™

CMRL Medium, no glutamine

CMRL medium was originally developed by Connaught Medical Research Laboratories for the growth of Earle's 'L'cells under serum-free conditions. CMRL자세히 알아보기
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카탈로그 번호수량
11530037500 mL
카탈로그 번호 11530037
제품 가격(KRW)
101,000
Online offer
Ends: 31-Dec-2025
106,000
할인액 5,000 (5%)
Each
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수량:
500 mL
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제품 가격(KRW)
101,000
Online offer
Ends: 31-Dec-2025
106,000
할인액 5,000 (5%)
Each
카트에 추가하기
CMRL medium was originally developed by Connaught Medical Research Laboratories for the growth of Earle's 'L'cells under serum-free conditions. CMRL medium is also especially useful for cloning monkey kidney cells and for growth of many other mammalian cell lines when supplemented with horse or calf serum.
This CMRL medium is manufactured as follows:
WithWithout
• Phenol Red• L-glutamine
• HEPES

The complete formulation is available.

Gibco™ CMRL medium is a modified version of Medium 199. CMRL medium is particularly rich in nucleosides and vitamins

Product Intended Use
For in vitro diagnostic use. CAUTION: Not for human or animal therapeutic use. Uses other than the labeled intended use may be a violation of local law.

Dual-Site cGMP Manufacturing
Gibco™ CMRL medium is manufactured at a cGMP compliant facility, located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards. For supply chain continuity, we offer an identical Gibco™ CMRL medium product made in our Scotland facility (21530-027). This facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standard.

CMRL medium contains no proteins or growth factors. Therefore, CMRL medium requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). CMRL medium uses a sodium bicarbonate buffer system (2.2 g/L) and therefore requires a 5-10% CO2 environment to maintain physiological pH.

This product is not used for in vitro diagnostic purpose in some countries.
For Research Use Only. Not for use in diagnostic procedures. Not for human or animal therapeutic use. Uses other than the labeled intended use may be a violation of local law. For in vitro diagnostic use.
사양
세포주Earle's 'L'cells
세포 유형Monkey Kidney Cells
농도1 X
제조 품질cGMP-compliant under the ISO 13485 standard
제품라인Gibco
제품 유형CMRL Medium
수량500 mL
유통 기한12 Months From Date of Manufacture
배송 조건Room Temperature
분류Animal Origin
형태Liquid
멸균Sterile-filtered
첨가제 포함Low Glucose, Phenol Red
첨가제 없음No Glutamine, No HEPES, No Sodium Pyruvate
Unit SizeEach
구성 및 보관
Storage conditions: 2-8°C. Protect from light
Shipping conditions: Ambient
Shelf life: 12 months from date of manufacture

자주 묻는 질문(FAQ)

How long can I keep my media after supplementing with serum?

Generally speaking, media can be used for up to three weeks after supplementation with serum. There are no formal studies to support this, but it is the rule of thumb used by our scientists.

Find additional tips, troubleshooting help, and resources within our Mammalian Cell Culture Basics Support Center.

My medium was shipped at room temperature but it is supposed to be stored refrigerated. Is it okay?

We routinely ship media that require long-term storage in the refrigerator at room temperature. We have done studies on representative media formulations to show that media can be at room temperature for up to a week without a problem.

Find additional tips, troubleshooting help, and resources within our Mammalian Cell Culture Basics Support Center.

How can I remove mycoplasma contamination from my cell culture medium?

Very often mycoplasma contamination cannot be removed from the culture so it should be discarded. You may have a unique culture that you prefer not to discard and would like to try to clean it. Ciprofloxacin and Plasmocin have reportedly been used for this application. If interested in a protocol or directions for use, check with the antibiotic supplier or published literature. Note that mycoplasma are very difficult to remove from culture and spread easily so the treated cultures should be quarantined until clear of mycoplasma, and your laboratory should be thoroughly cleaned.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

I see a decrease in growth of my culture. What should I do?

Try changing the medium or serum. Compare media formulations for differences in glucose, amino acids, and other components. Compare an old lot of serum with a new lot. Increase initial cell inoculums. Lastly, adapt cells sequentially to new medium.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

My cells are not adhering to the culture vessel. What should I do?

This can occur if cells are overly trypsinized. Trypsinize for a shorter time or use less trypsin. Mycoplasma contamination could also cause this problem. Segregate your culture and test for mycoplasma infection. Lastly, check for attachment factors in the medium.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.