Lipofectamine™ 2000 Transfection Reagent
Lipofectamine™ 2000 Transfection Reagent
인용 및 참조 문헌 (55)
Invitrogen™

Lipofectamine™ 2000 Transfection Reagent

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Lipofectamine™ 2000 Transfection Reagent 는 폭넓은 부착성과 현탁 세포주에 대해 효과적인 능력을 입증한 적용성 높은 transfection reagent 입니다. 연구자들은 Lipofectamine™자세히 알아보기
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카탈로그 번호수량
1166850015 mL
116680191.5 mL
116680270.75 mL
116680300.3 mL
카탈로그 번호 11668500
제품 가격(KRW)
8,145,000
キャンペーン価格
Ends: 31-Dec-2025
9,049,000
할인액 904,000 (10%)
Each
카트에 추가하기
수량:
15 mL
제품 가격(KRW)
8,145,000
キャンペーン価格
Ends: 31-Dec-2025
9,049,000
할인액 904,000 (10%)
Each
카트에 추가하기
Lipofectamine™ 2000 Transfection Reagent 는 폭넓은 부착성과 현탁 세포주에 대해 효과적인 능력을 입증한 적용성 높은 transfection reagent 입니다. 연구자들은 Lipofectamine™ 2000 Reagent를 siRNA 및 shRNA 기반 유전자 knockdown 실험과 유전자 발현 시험에 사용합니다. Lipofectamine™ 2000는 siRNA 와 plasmid DNA를 co-transfection하는 라이프 테크놀로지스 최고의 시약입니다.

Lipofectamine™ 2000 Reagent의 특징:
• 다양한 세포주에 대한 뛰어난 transfection efficiency, 최고의 재조합 단백질 발현(표 참조)
• 우수한 siRNA-plasmid DNA co-transfection 능력
• 혈청 함유 배지에서 입증된 유효성 - transfection 후 배지를 바꿀 필요가 없습니다.
• 높은 처리양 적용시 믿을 수 있는 성능
• stable cell line 준비를 위한 최고의 선택

유전자 발현 및 Gene Silencing 을 위한 최고의 Transfection Reagent
Lipofectamine™ 2000 Reagent는 일반적인 모든 세포주는 물론이고 transfection efficiency가 떨어지는 것으로 알려진 세포주에도 효과적입니다. 혈청 함유 배지와 무혈청 배지에 모두 사용가능합니다. gene silencing 시에도 Lipofectamine™ 2000 Reagent는 뛰어난 transfection efficiency로 확실한 결과를 보장합니다. Lipofectamine™ 2000 Reagent는 siRNA와 plasmid DNA 을 위한 co-transfection에 효과가 입증된 최고의 시약입니다. Lipofectamine™ 2000 Reagent는 사용이 용이합니다– 핵산과의 혼합 및 세포 배양에 첨가가 간단합니다.

고수율 작업에 이상적임
간편한 사용, 빠른 속도, 높은 형질감염 효율로 Lipofectamine™ 2000 Reagent는 일시적인 단백질 발현, 고수율 RNAi transfection에 모두 이상적입니다. transfection condition은 이와 같은 어플리케이션에 사용되는 자동화 로봇 시스템에 쉽게 부합이 가능합니다. Lipofectamine™ 2000 프로토콜, 인용, 추가 제품 설명은 세포주 데이터베이스(Cell Lines Database)를 참조하세요.

이는 연구 전용 제품입니다. 치료 또는 진단 목적으로 동물이나 인간에 사용할 수 없습니다.
For Research Use Only. Not for use in diagnostic procedures.
사양
용도(애플리케이션)Transfection
그린 기능Sustainable packaging
고처리량 호환성High-throughput Compatible
제품라인Lipofectamine
제품 유형Transfection Reagent
수량15 mL
혈청 호환 가능Yes
배송 조건Approved for shipment at Room Temperature or on Wet Ice
셀 유형Established Cell Lines, Stem Cells, Primary Cells, Hard-to-Transfect Cells
형식6-well Plate, 12-well Plate, 24-well Plate, 48-well Plate, 96-well Plate, Flasks
샘플 종류Plasmid DNA, Synthetic siRNA, RNAi Plasmids (shRNA, miR)
Transfection TechniqueLipid-based Transfection
Unit SizeEach
구성 및 보관
Contains one bottle (15 mL) Lipofectamine™ 2000 Reagent. Store at 2–8°C. Do not freeze.

자주 묻는 질문(FAQ)

I transfected GFP into cells using Lipofectamine 2000 and saw a light granular orange background fluorescence. What could be causing this?

Transfection with cationic lipids can produce light granular orange background fluorescence. The orange fluorescence is associated with the lipid/DNA complexes and is not related to GFP. This background varies depending on the cationic lipid reagent used and does not interfere with transfection results. If desired, try performing fluorescence imaging in PBS instead of culture medium. Also, make sure the cells are healthy and intact as lysed cells or cells under stress could generate autofluorescence products.

Find additional tips, troubleshooting help, and resources within our Transfection Support Center.

I accidentally left my lipid reagent at room temperature. Can I still use it?

Yes, all of our lipid transfection reagents are stable at room temperature for months.

Find additional tips, troubleshooting help, and resources within our Lipid-Based Transfection Support Center.

Can I use Lipofectamine RNAiMAX to co-transfect siRNA with plasmid DNA?

While Lipofectamine 3000 or Lipofectamine 2000 may be used for co-transfection of siRNA with plasmid DNA, Lipofectamine RNAiMAX cannot be used.

Find additional tips, troubleshooting help, and resources within our Transfection Support Center.

Can I use Lipofectamine 2000 to co-transfect plasmids and siRNA?

You can co-transfect plasmids and siRNA using Lipofectamine 2000. Please click here (http://www.thermofisher.com/us/en/home/references/protocols/cell-culture/transfection-protocol/plasmid-co-transfection-protocol-lipofectamine-sirna.html) for protocol information.

Find additional tips, troubleshooting help, and resources within our Transfection Support Center.

For how long is the Lipofectamine 2000:DNA complex stable?

Lipofectamine 2000:DNA complexes are stable for 6 hours at room temperature when the complex is prepared in Opti-MEM medium. Use of other media for complex formation may decrease stability.

Find additional tips, troubleshooting help, and resources within our Transfection Support Center.

View all Lipofectamine™ 2000 Transfection Reagent, 15 mL FAQs

인용 및 참조 문헌 (55)

인용 및 참조 문헌
Abstract
Non-viral-mediated gene transfer of OX40 ligand for tumor immunotherapy.
Authors:Rakitina OA,Kuzmich AI,Bezborodova OA,Kondratieva SA,Pleshkan VV,Zinovyeva MV,Didych DA,Sass AV,Snezhkov EV,Kostina MB,Koksharov MO,Alekseenko IV
Journal:Frontiers in immunology
PubMed ID:39007148
BACKGROUND: Immune checkpoint blockade (ICB) is rapidly becoming a standard of care in the treatment of many cancer types. However, the subset of patients who respond to this type of therapy is limited. Another way to promote antitumoral immunity is the use of immunostimulatory molecules, such as cytokines or T ... More
Mammalian cell penetration, siRNA transfection, and DNA transfection by supercharged proteins.
Authors:McNaughton BR, Cronican JJ, Thompson DB, Liu DR,
Journal:Proc Natl Acad Sci U S A
PubMed ID:19307578
'Nucleic acid reagents, including small interfering RNA (siRNA) and plasmid DNA, are important tools for the study of mammalian cells and are promising starting points for the development of new therapeutic agents. Realizing their full potential, however, requires nucleic acid delivery reagents that are simple to prepare, effective across many ... More
Amino acid residues critical for endoplasmic reticulum export and trafficking of platelet-activating factor receptor.
Authors:Hirota N, Yasuda D, Hashidate T, Yamamoto T, Yamaguchi S, Nagamune T, Nagase T, Shimizu T, Nakamura M,
Journal:J Biol Chem
PubMed ID:20007715
'Several residues are conserved in the transmembrane domains (TMs) of G-protein coupled receptors. Here we demonstrate that a conserved proline, Pro(247), in TM6 of platelet-activating factor receptor (PAFR) is required for endoplasmic reticulum (ER) export and trafficking after agonist-induced internalization. Alanine-substituted mutants of the conserved residues of PAFRs, including P247A, ... More
Caveolin regulates endocytosis of the muscle repair protein, dysferlin.
Authors:Hernández-Deviez DJ, Howes MT, Laval SH, Bushby K, Hancock JF, Parton RG,
Journal:J Biol Chem
PubMed ID:18096699
'Dysferlin and Caveolin-3 are plasma membrane proteins associated with muscular dystrophy. Patients with mutations in the CAV3 gene show dysferlin mislocalization in muscle cells. By utilizing caveolin-null cells, expression of caveolin mutants, and different mutants of dysferlin, we have dissected the site of action of caveolin with respect to dysferlin ... More
Functional analysis of FSP27 protein regions for lipid droplet localization, caspase-dependent apoptosis, and dimerization with CIDEA.
Authors:Liu K, Zhou S, Kim JY, Tillison K, Majors D, Rearick D, Lee JH, Fernandez-Boyanapalli RF, Barricklow K, Houston MS, Smas CM,
Journal:Am J Physiol Endocrinol Metab
PubMed ID:19843876
'The adipocyte-specific protein FSP27, also known as CIDEC, is one of three cell death-inducing DFF45-like effector (CIDE) proteins. The first known function for CIDEs was promotion of apoptosis upon ectopic expression in mammalian cells. Recent studies in endogenous settings demonstrated key roles for CIDEs in energy metabolism. FSP27 is a ... More
View all Lipofectamine™ 2000 Transfection Reagent, 15 mL citations