MEM, autoclavable, no glutamine, powder

인용 및 참조 문헌 (1)

Gibco™

MEM, autoclavable, no glutamine, powder

Minimum Essential Medium (MEM) is one of the most commonly used of all cell culture media. MEM can be used자세히 알아보기

카탈로그 번호	수량
11700077	10 L

카탈로그 번호 11700077

제품 가격(KRW)

70,000

キャンペーン価格

Ends: 31-Mar-2026

77,000

할인액 7,000 (9%)

카트에 추가하기

Customize this product

제품 가격(KRW)

70,000

キャンペーン価格

Ends: 31-Mar-2026

77,000

할인액 7,000 (9%)

카트에 추가하기

Minimum Essential Medium (MEM) is one of the most commonly used of all cell culture media. MEM can be used with a variety of suspension and adherent mammalian cells, including HeLa, BHK-21, 293, HEP-2, HT-1080, MCF-7, fibroblasts, and primary rat astrocytes. We offer a variety of Gibco MEM modifications for a range of cell culture applications. Find the right formulation using the media selector tool.

This MEM is modified as follows:

With: Phenol Red, Earle's salts

Without: L-glutamine, HEPES, Sodium Bicarbonate

The complete formulation is available.

Gibco MEM, developed by Harry Eagle, was based on his earlier formulation of Basal Medium Eagle (BME). Many other modifications of MEM followed, including Glasgow's MEM, MEM α, DMEM, and Temin's Modification. To allow autoclaving this medium, choline chloride is substituted with choline bitartrate, phenol red is reduced, and succinic acid is added to prevent precipitation at low pH. This MEM formulation contains Earle's salts for use in a CO₂ incubator. This product is made with Earle's salts.

MEM contains no proteins, lipids, or growth factors. Therefore, MEM requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). MEM uses a sodium bicarbonate buffer system (2.2 g/L) and therefore requires a 5–10% CO₂ environment to maintain physiological pH.

This autoclavable form of Gibco™ cell culture medium requires pH adjustment to 4.1–4.2 before autoclaving, following by sodium bicarbonate and L-glutamine supplementation and final pH adjustment to 7.2–7.4 (see protocol for details).

Please note that the shelf life on delivered products varies. This product has a minimum shelf life of 6 months at time of shipment. For more information regarding product shelf life, including details on specific product lots, please contact Customer Service.

Not for human or animal therapeutic use. Uses other than the intended use may be a violation of local law. For in vitro diagnostic use.

세포주HeLa, BHK-21, 293, HEP-2, HT-1080, MCF-7, and fibroblasts

세포 유형Primary Rat Astrocytes

제조 품질cGMP-compliant under the ISO 13485 standard

제품라인Gibco

제품 유형MEM (Minimum Essential Medium)

수량10 L

유통 기한36 Months From Date of Manufacture

배송 조건Room Temperature

분류Animal Origin-free

형태Powder

Serum LevelStandard Serum Supplementation

멸균Sterile-filtered

첨가제 포함Phenol Red, Earle's Balanced Salt Solution (EBSS)

첨가제 없음No HEPES, No Sodium Bicarbonate

Unit SizeEach

구성 및 보관

Storage conditions: 2-8°C
Shipping conditions: Ambient
Shelf life: 36 months from date of manufacture

자주 묻는 질문(FAQ)

Where can I find the osmolality for MEM Medium?

The osmolality is listed in the COA for the particular lot number of the medium.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

What is the shelf life of my powdered media once I reconstitute it?

Expiration date of most Gibco reconstituted dry format media (AGT or DPM) has not been established; end users should assess performance and stability of this reconstituted media in a system that is relevant to their process. A dry format product (either DPM or AGT) will age upon storage and while we may not be able to detect which component(s) degrade since we can't measure every component, that doesn't mean that the reconstituted liquid will have the same stability as a fresh liquid made by direct weigh methods.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

How long can I keep my media after supplementing with serum?

Generally speaking, media can be used for up to three weeks after supplementation with serum. There are no formal studies to support this, but it is the rule of thumb used by our scientists.

Find additional tips, troubleshooting help, and resources within our Mammalian Cell Culture Basics Support Center.

My medium was shipped at room temperature but it is supposed to be stored refrigerated. Is it okay?

We routinely ship media that require long-term storage in the refrigerator at room temperature. We have done studies on representative media formulations to show that media can be at room temperature for up to a week without a problem.

Find additional tips, troubleshooting help, and resources within our Mammalian Cell Culture Basics Support Center.

How can I remove mycoplasma contamination from my cell culture medium?

Very often mycoplasma contamination cannot be removed from the culture so it should be discarded. You may have a unique culture that you prefer not to discard and would like to try to clean it. Ciprofloxacin and Plasmocin have reportedly been used for this application. If interested in a protocol or directions for use, check with the antibiotic supplier or published literature. Note that mycoplasma are very difficult to remove from culture and spread easily so the treated cultures should be quarantined until clear of mycoplasma, and your laboratory should be thoroughly cleaned.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

View all MEM, autoclavable, no glutamine, powder FAQs

인용 및 참조 문헌 (1)

인용 및 참조 문헌

Abstract

Novel polyaminolipids enhance the cellular uptake of oligonucleotides.

Authors: Guy-Caffey J K; Bodepudi V; Bishop J S; Jayaraman K; Chaudhary N;

Journal:J Biol Chem

PubMed ID:8537413

'Two new polyaminolipids have been synthesized for the purpose of improving cellular uptake of oligonucleotides. The amphipathic compounds are conjugates of spermidine or spermine linked through a carbamate bond to cholesterol. The polyaminolipids are relatively nontoxic to mammalian cells. In tissue culture assays, using fluorescent-tagged or radiolabeled triple helix-forming oligonucleotides, ... More