Glasgow's MEM (GMEM) was originally developed by Ian McPherson and Michael Stoker as a modification of Eagle's Minimal Essential Medium.자세히 알아보기
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11710035
500 mL
카탈로그 번호 11710035
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제품 가격(KRW)
77,000
Online offer
Ends: 31-Mar-2026
85,000
할인액 8,000 (9%)
Each
카트에 추가하기
Glasgow's MEM (GMEM) was originally developed by Ian McPherson and Michael Stoker as a modification of Eagle's Minimal Essential Medium. It was used to study the genetic factors that affected cell competence. Glasgow's MEM was developed for use with kidney cell lines, such as BHK-21.
Using Glasgow's MEM Glasgow's MEM is unique from other media as it contains twice the concentration of amino acids and vitamins compared to the original Basal Medium Eagle, and is used without serum. Glasgow's MEM was originally formulated with 10% tryptose phosphate broth. Glasgow's MEM contains no proteins, lipids, or growth factors. Therefore, Glasgow's MEM requires supplementation with 10% tryptose phosphate broth. Glasgow's MEM uses a sodium bicarbonate buffer system (2.75 g/L), and therefore requires a 5–10% CO2 environment to maintain physiological pH.
Dual-site cGMP manufacturing Glasgow's MEM is manufactured at a cGMP-compliant facility located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards. For supply chain continuity, we offer an identical Glasgow's MEM product made in our Scotland facility (21710-025). This facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standard.
This product is not used for in vitro diagnostic purpose in some countries.
For Research Use Only. Not for use in diagnostic procedures.
사양
세포주BHK-21
농도1 X
제조 품질cGMP-compliant under the ISO 13485 standard
제품라인Gibco
제품 유형MEM (Minimum Essential Medium)
수량500 mL
유통 기한12 Months From Date of Manufacture
배송 조건Room Temperature
분류Animal Origin-free
형태Liquid
멸균Sterile-filtered
첨가제 포함High Glucose, Glutamine, Phenol Red
첨가제 없음No HEPES, No Sodium Pyruvate, No Tryptose Phosphate Broth
Unit SizeEach
구성 및 보관
Storage conditions: 2°C to 8°C (protect from light) Shipping conditions: Ambient Shelf life: 12 months from date of manufacture
자주 묻는 질문(FAQ)
How long can I keep my media after supplementing with serum?
Generally speaking, media can be used for up to three weeks after supplementation with serum. There are no formal studies to support this, but it is the rule of thumb used by our scientists.
My medium was shipped at room temperature but it is supposed to be stored refrigerated. Is it okay?
We routinely ship media that require long-term storage in the refrigerator at room temperature. We have done studies on representative media formulations to show that media can be at room temperature for up to a week without a problem.
How can I remove mycoplasma contamination from my cell culture medium?
Very often mycoplasma contamination cannot be removed from the culture so it should be discarded. You may have a unique culture that you prefer not to discard and would like to try to clean it. Ciprofloxacin and Plasmocin have reportedly been used for this application. If interested in a protocol or directions for use, check with the antibiotic supplier or published literature. Note that mycoplasma are very difficult to remove from culture and spread easily so the treated cultures should be quarantined until clear of mycoplasma, and your laboratory should be thoroughly cleaned.
I see a decrease in growth of my culture. What should I do?
Try changing the medium or serum. Compare media formulations for differences in glucose, amino acids, and other components. Compare an old lot of serum with a new lot. Increase initial cell inoculums. Lastly, adapt cells sequentially to new medium.
My cells are not adhering to the culture vessel. What should I do?
This can occur if cells are overly trypsinized. Trypsinize for a shorter time or use less trypsin. Mycoplasma contamination could also cause this problem. Segregate your culture and test for mycoplasma infection. Lastly, check for attachment factors in the medium.