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인용 및 참조 문헌 (1)
Invitrogen™

Platinum™ Quantitative PCR SuperMix-UDG

**********************Alternative Product: Try TaqMan Fast Advanced Master Mix, our highest-performance, probe-based master mix. With TaqMan Fast Advanced Master Mix, we’ve자세히 알아보기
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카탈로그 번호반응 수
11730025500 Reactions
11730017100 Reactions
카탈로그 번호 11730025
제품 가격(KRW)
2,322,000
キャンペーン価格
Ends: 30-Jun-2026
2,579,000
할인액 257,000 (10%)
Each
반응 수:
500 Reactions
제품 가격(KRW)
2,322,000
キャンペーン価格
Ends: 30-Jun-2026
2,579,000
할인액 257,000 (10%)
Each
**********************
Alternative Product: Try TaqMan Fast Advanced Master Mix, our highest-performance, probe-based master mix. With TaqMan Fast Advanced Master Mix, we’ve taken the best of Platinum Quantitative PCR SuperMix and added additional capabilities for your gene expression analysis.

**********************
Platinum™ Quantitative PCR SuperMix-UDG is a ready-to-use mixture for high-specificity, real-time quantitative DNA amplification, containing UDG to prevent carryover contamination (1,2). The system is optimized to provide superior performance with either LUX™ Fluorogenic Primers or probe-based detection systems.

Platinum™ Quantitative PCR SuperMix-UDG:

• Is highly sensitive and specific
• Allows room temperature reaction assembly (3)
• Is easy to use-just add template and fluorogenic primers or probes for detection
• Includes a vial of 50 mM MgCl2 to accommodate a variety of primer/template PCR systems
• Is compatible with most real-time instruments

Applications: Real-time quantitative amplification of DNA from complex genomic, viral, and plasmid templates, RT-PCR, and fluorometric SNP (Single Nucleotide Polymorphism) detection.
For Research Use Only. Not for use in diagnostic procedures.
사양
용도(장비)7500 System, BioRad iCycler iQ, BioRad iQ5, Stratagene Mx4000, MJ Chromo4, MJ Opticon, Stratagene Mx3000P, Stratagene Mx3005P, Cepheid SmartCycler, BioRad MyiQ
반응 수500 Reactions
제품라인Platinum
제품 유형Quantitative PCR SuperMix-UDG
수량500 reactions
샘플 종류DNA (Genomic), cDNA
배송 조건Dry Ice
충분500 Reactions
검출 방법Primer-probe
용도(애플리케이션)Gene Expression
GC-Rich PCR PerformanceHigh
PCR 방법qPCR
반응 속도Standard
Unit SizeEach
구성 및 보관
Platinum™ Quantitative PCR SuperMix-UDG is supplied in a 2X concentration [40 mM Tris-HCl (pH 8.4), 100 mM KCl, 6 mM MgCl2 , 400 μM dATP, 400 μM dCTP, 400 μM dGTP, 800 μM dUTP, 40 units/ml UDG, 60 units/ml Platinum™ Taq DNA Polymerase, stabilizers]. A vial of 50 mM MgCl2 is also provided. Store at -20°C. Guaranteed stable for 6 months when properly stored.

자주 묻는 질문(FAQ)

I want to perform qRT-PCR using cDNA that I synthesize directly from cells. Can I use the SuperScript III CellsDirect cDNA Kit for end-point PCR and the Platinum Quantitative PCR SuperMix-UDG kit?

We would recommend using our Cells-to-CT kits for best qRT-PCR results. The CellsDirect kits for end-point PCR have not been optimized for qRT-PCR.

Why is UDG important in the Platinum Quantitative PCR SuperMix-UDG mix?

UDG and dUTP are included to prevent the reamplification of carryover PCR products between reactions. dUTP ensures that any amplified DNA will contain uracil, while UDG removes uracil residues from single- or double-stranded DNA, preventing dU-containing DNA from serving as template in future PCRs.

인용 및 참조 문헌 (1)

인용 및 참조 문헌
Abstract
Development and validation of a rotor-gene real-time PCR assay for detection, identification, and quantification of Chlamydia trachomatis in a single reaction.
Authors:Jalal H, Stephen H, Curran MD, Burton J, Bradley M, Carne C,
Journal:J Clin Microbiol
PubMed ID:16390971
A multitarget real-time PCR (MRT-PCR) for detection of Chlamydia trachomatis DNA was developed and validated. There were three targets for amplification in a single reaction: the cryptic plasmid (CP), the major outer membrane protein (MOMP) gene, and an internal control. The assay had the following characteristics: (i) detection and confirmation ... More