SuperScript™ III Platinum™ SYBR™ Green One-Step qRT-PCR Kit
SuperScript™ III Platinum™ SYBR™ Green One-Step qRT-PCR Kit
Actual product may vary
SuperScript™ III Platinum™ SYBR™ Green One-Step qRT-PCR Kit
SuperScript™ III Platinum™ SYBR™ Green One-Step qRT-PCR Kit
Invitrogen™

SuperScript™ III Platinum™ SYBR™ Green One-Step qRT-PCR Kit

The SuperScript™ III Platinum™ One-Step qRT-PCR Kits combine the most powerful reverse transcriptase and DNA polymerase technologies to deliver precise자세히 알아보기
Have Questions?
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카탈로그 번호반응 수
11736059500 Reactions
11736051100 Reactions
카탈로그 번호 11736059
제품 가격(KRW)
3,741,000
Each
카트에 추가하기
반응 수:
500 Reactions
제품 가격(KRW)
3,741,000
Each
카트에 추가하기
The SuperScript™ III Platinum™ One-Step qRT-PCR Kits combine the most powerful reverse transcriptase and DNA polymerase technologies to deliver precise and accurate analysis of gene expression in a convenient one-step format. The SuperScript™ III Platinum™ One-Step qRT-PCR Kit provides sensitive, specific detection with fluorogenic primers and probes, while the SuperScript™ III Platinum™ SYBR™ Green One-Step qRT-PCR Kit offers the ease and convenience of SYBR™ Green I detection

SuperScript™ III Platinum™ One-Step qRT-PCR Kits provide:

• SuperScript™ III RT for higher temperature cDNA synthesis for greater success with difficult RNA secondary structure
• Platinum™ Taq DNA Polymerase with hot-start technology for improved specificity
• LUX™ Fluorogenic Primers (Figure 1) or dual-labeled fluorogenic probes (Figure 2) for superior detection performance
• SYBR™ Green I dye for easy and convenient detection (Figure 3)
• Validated performance on multiple real-time instrument platforms, including rotor-based systems
For Research Use Only. Not for use in diagnostic procedures.
사양
용도(장비)7500 System, BioRad iCycler iQ, BioRad iQ5, Stratagene Mx4000, MJ Chromo4, MJ Opticon, Stratagene Mx3000P, Stratagene Mx3005P, Cepheid SmartCycler, BioRad MyiQ
반응 수500 Reactions
중합효소Taq DNA Polymerase
제품라인Platinum, SYBR, SuperScript
제품 유형One-Step qRT-PCR Kit
수량500 reactions
샘플 종류RNA
배송 조건Dry Ice
충분500 Reactions
검출 방법SYBR
용도(애플리케이션)Real Time PCR (qPCR)
PCR 방법1-step RT-qPCR
반응 속도Standard
Unit SizeEach
구성 및 보관
• 500 μL SuperScript™ III RT/Platinum™ Taq Mix
• 12.5 mL 2X SYBR™ Green Reaction Mix
• 2 × 1 mL Magnesium Sulfate (50 mM)
• 500 μL ROX Reference Dye (25 μM)
• 1.3 mL 20X Bovine Serum Albumin (1 mg/mL)

Store all components at -20°C (-80°C for long-term storage). ROX Reference Dye must be stored in the dark.

자주 묻는 질문(FAQ)

What can I do to improve the sensitivity of my qPCR assay?

If you are targeting a low-abundance gene, you may have trouble getting Ct values in a good, reliable range (Ct > 32). To increase the sensitivity of the assay, you may want to consider the following:

- Increase the amount of RNA input into your reverse transcription reaction, if possible
- Increase the amount of cDNA in your qPCR reaction (20% by volume max)
- Try a different reverse transcription kit, such as our SuperScript VILO Master Mix, for the highest cDNA yield possible
- Consider trying a one-step or Cells-to-CT type workflow (depending on your sample type)

How do I set the baseline for my qPCR experiment?

Most times your instrument software can automatically set a proper baseline for your data. Check out our short video, Understanding Baselines, for more information on how to set them (https://www.youtube.com/watch?feature=player_embedded&v=5BjFAJHW-bE).

How do I set the threshold for my qPCR experiment?

In most cases your instrument software can automatically set a proper threshold for your data. Check out our short video, Understanding Thresholds, for more information on how to set them (https://www.youtube.com/watch?feature=player_embedded&v=H_xsuRQIM9M).

I am not getting any amplification with my TaqMan Assay or SYBR Green primer set. What is causing this?

There could be several reasons for no amplification from an assay or primer set. Please see these examples and suggested solutions in our Real-Time Troubleshooting Tool (https://www.thermofisher.com/us/en/home/life-science/pcr/real-time-pcr/qpcr-education/real-time-pcr-troubleshooting-tool/gene-expression-quantitation-troubleshooting/no-amplification.html) for more details.

I am getting amplification in my no-template control (NTC) wells in my qPCR experiment. What is causing this?

There could be several reasons for amplification in a NTC well. Please see these examples and suggested solutions in our Real-Time Troubleshooting Tool (https://www.thermofisher.com/us/en/home/life-science/pcr/real-time-pcr/qpcr-education/real-time-pcr-troubleshooting-tool/gene-expression-quantitation-troubleshooting/amplification-no-template-control.html) for more details.