SuperScript™ III Platinum™ SYBR™ Green One-Step qPCR Kit w/ROX
Invitrogen™
SuperScript™ III Platinum™ SYBR™ Green One-Step qPCR Kit w/ROX
Our One-Step qRT-PCR Kits with ROX combine the most powerful reverse transcriptase and DNA polymerase technologies to deliver precise and자세히 알아보기
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카탈로그 번호
반응 수
11746500
500 Reactions
11746100
100 Reactions
2 개 옵션
카탈로그 번호 11746500
제품 가격(KRW)
3,337,000
Online offer
Ends: 31-Dec-2025
3,707,000
할인액 370,000 (10%)
Each
카트에 추가하기
반응 수:
500 Reactions
제품 가격(KRW)
3,337,000
Online offer
Ends: 31-Dec-2025
3,707,000
할인액 370,000 (10%)
Each
카트에 추가하기
Our One-Step qRT-PCR Kits with ROX combine the most powerful reverse transcriptase and DNA polymerase technologies to deliver precise and accurate analysis of gene expression in a convenient, high-throughput one-step format. One-Step qRT-PCR kits with ROX include:
–SuperScript™ III Reverse Transcriptase: produces higher yields of full-length cDNA for broader gene representation
–Platinum™ Taq DNA Polymerase: activates faster and more completely than other hot-start enzymes to deliver more sensitive amplification (Figure 1) –ROX reference dye premixed at optimal concentration for ABI instruments
Two kit configurations allow your choice of detection methods:
–SuperScript™ III Platinum™ SYBR™ Green One-Step qRT-PCR Kit provides a highly efficient, cost-effective qRT-PCR system with SYBR Green I detection –SuperScript™ III Platinum™ One-Step qRT-PCR Kit provides sensitive, specific detection for use with LUX™ fluorogenic primers or dual-labeled probes (e.g. TaqMan™) (Figure 2)
Contents and Storage: SuperScript™ III Platinum™ SYBR™ GreenOne-Step qRT-PCR Kit with ROX includes SuperScript™ III RT/Platinum™ Taq Mix, 2X Reaction Mix (containing SYBR™ Green I, ROX Reference Dye, dNTPs, and MgSO4), and 50 mM MgSO4. SuperScript™ III Platinum™One-Step qRT-PCR Kit with ROX includes SuperScript™ III RT/Platinum™ Taq Mix, 2X Reaction Mix (containing ROX Reference Dye, dNTPs and MgSO4), and 50 mM MgSO4. Store all components at -20°C. Store ROX Reference dye in the dark. Guaranteed stable for 6 months when properly stored.
For Research Use Only. Not for use in diagnostic procedures.
사양
용도(장비)7000 System, 7300 System, 7700 System, 7900HT System
반응 수500 Reactions
중합효소Taq DNA Polymerase
제품라인Platinum, SYBR, SuperScript
제품 유형Real Time PCR SYBR Master Mix
수량500 reactions
샘플 종류RNA
배송 조건Dry Ice
충분500 Reactions
농도2X
검출 방법SYBR
용도(애플리케이션)Gene Expression
PCR 방법1-step RT-qPCR
반응 속도Standard
Unit SizeEach
구성 및 보관
Store in freezer (-5 to -30°C).
자주 묻는 질문(FAQ)
What can I do to improve the sensitivity of my qPCR assay?
If you are targeting a low-abundance gene, you may have trouble getting Ct values in a good, reliable range (Ct > 32). To increase the sensitivity of the assay, you may want to consider the following:
- Increase the amount of RNA input into your reverse transcription reaction, if possible
- Increase the amount of cDNA in your qPCR reaction (20% by volume max)
- Try a different reverse transcription kit, such as our SuperScript VILO Master Mix, for the highest cDNA yield possible
- Consider trying a one-step or Cells-to-CT type workflow (depending on your sample type)
How do I set the baseline for my qPCR experiment?
Most times your instrument software can automatically set a proper baseline for your data. Check out our short video, Understanding Baselines, for more information on how to set them (https://www.youtube.com/watch?feature=player_embedded&v=5BjFAJHW-bE).
How do I set the threshold for my qPCR experiment?
In most cases your instrument software can automatically set a proper threshold for your data. Check out our short video, Understanding Thresholds, for more information on how to set them (https://www.youtube.com/watch?feature=player_embedded&v=H_xsuRQIM9M).
I am not getting any amplification with my TaqMan Assay or SYBR Green primer set. What is causing this?
There could be several reasons for no amplification from an assay or primer set. Please see these examples and suggested solutions in our Real-Time Troubleshooting Tool (https://www.thermofisher.com/us/en/home/life-science/pcr/real-time-pcr/qpcr-education/real-time-pcr-troubleshooting-tool/gene-expression-quantitation-troubleshooting/no-amplification.html) for more details.
I am getting amplification in my no-template control (NTC) wells in my qPCR experiment. What is causing this?
There could be several reasons for amplification in a NTC well. Please see these examples and suggested solutions in our Real-Time Troubleshooting Tool (https://www.thermofisher.com/us/en/home/life-science/pcr/real-time-pcr/qpcr-education/real-time-pcr-troubleshooting-tool/gene-expression-quantitation-troubleshooting/amplification-no-template-control.html) for more details.