SuperScript™ VILO™ Master Mix
해당 제품을 구매한 고객들이 함께 검색한 제품을 추천해드립니다. 온라인 할인 진행 중!
SuperScript™ VILO™ Master Mix
인용 및 참조 문헌 (1)
Invitrogen™

SuperScript™ VILO™ Master Mix

SuperScript VILO Master Mix is a first-strand cDNA synthesis master mix for two-step RT-qPCR applications. The master mix combines SuperScript자세히 알아보기
Have Questions?
보기 방식 변경buttonViewtableView
카탈로그 번호반응 수
11755250250 Reactions
1175505050 Reactions
11755500500 Reactions
카탈로그 번호 11755250
제품 가격(KRW)
3,621,000
Online offer
Ends: 31-Dec-2025
4,260,000
할인액 639,000 (15%)
Each
카트에 추가하기
반응 수:
250 Reactions
대량 주문 또는 맞춤형 요청
제품 가격(KRW)
3,621,000
Online offer
Ends: 31-Dec-2025
4,260,000
할인액 639,000 (15%)
Each
카트에 추가하기

SuperScript VILO Master Mix is a first-strand cDNA synthesis master mix for two-step RT-qPCR applications. The master mix combines SuperScript III Reverse Transcriptase (RT) and other RT reaction components in a single-tube format to enable reliable first-strand synthesis and high cDNA yields from a wide range of input RNA concentrations. The high RT efficiency of SuperScript III RT and the optimized formulation allow the master mix to deliver high linearity in cDNA synthesis across a broad range of input RNA template for two-step quantitative RT-PCR assays.

Features of SuperScript VILO Master Mix include:
• Starting material can range up to 2.5 μg of total RNA in a 20 μL cDNA synthesis reaction
• Recombinant ribonuclease inhibitor safeguards against the degradation of target RNA due to ribonuclease contamination
• RT is engineered with reduced RNase H activity and increased thermal stability

For superior cDNA synthesis performance in RT-qPCR applications
SuperScript IV VILO Master Mix elevates the trusted VILO technology to the next level with the highly processive and thermostable SuperScript IV RT and further optimized buffer. The master mix enables efficient cDNA synthesis at higher temperatures in less time and provides superior cDNA yield and sensitivity even with suboptimal purity or scarce templates.

For Research Use Only. Not for use in diagnostic procedures.
사양
최종 제품 유형First-Strand cDNA
형식Master Mix
반응 수250 Reactions
최적 반응 온도42°C
수량250 reactions
반응 형식Master Mix
시약 유형Reverse Transcription
역전사 효소SuperScript III
리보뉴클레아제 H 활성Reduced
배송 조건Dry Ice
크기(최종 제품)Up to 10 kb
시작 물질RNA
기술Reverse Transcription
농도5X
검출 방법qPCR
용도(애플리케이션)Real Time PCR (qPCR)
GC-Rich PCR PerformanceHigh
반응 속도60 min.
Unit SizeEach
구성 및 보관

• SuperScript VILO Master Mix (1 mL)

Store at –5 to –30°C.

자주 묻는 질문(FAQ)

What can I do to improve the sensitivity of my qPCR assay?

If you are targeting a low-abundance gene, you may have trouble getting Ct values in a good, reliable range (Ct > 32). To increase the sensitivity of the assay, you may want to consider the following:

- Increase the amount of RNA input into your reverse transcription reaction, if possible
- Increase the amount of cDNA in your qPCR reaction (20% by volume max)
- Try a different reverse transcription kit, such as our SuperScript VILO Master Mix, for the highest cDNA yield possible
- Consider trying a one-step or Cells-to-CT type workflow (depending on your sample type)

What is the difference between SuperScript III RT and the RT in the SuperScript VILO kit?

The SuperScript VILO cDNA Synthesis Kit contains a mix of SuperScript III RT and helper proteins which help to increase the efficiency of the reverse transcription reaction and thus improve yield. The RT in the SuperScript

The SuperScript VILO cDNA Synthesis Kit (Cat. No. 11754050) contains a mix of SuperScript III RT and helper proteins which help to increase the efficiency of the reverse transcription reaction and thus improve yield. The RT in the SuperScript VILO kit is active at 42 degrees C due to the helper proteins.

How much of the first-strand cDNA reaction should I load for PCR?

While the volume is dependent on the starting amount of RNA used for the first-strand synthesis and the abundance of the target gene, we'd recommend starting with 10% of the first-strand reaction for your PCR reaction.

인용 및 참조 문헌 (1)

인용 및 참조 문헌
Abstract
Hypothalamic distribution of somatostatin mRNA expressing neurones relative to pubertal and adult changes in pulsatile growth hormone secretion in mice.
Authors:Tan HY, Huang L, Simmons D, Veldhuis JD, Steyn FJ, Chen C
Journal:
PubMed ID:23855876
'The age-associated decline in growth hormone (GH) secretion may be a consequence of the reduction in the number of GH-releasing hormone (GHRH) positive neurones. However, it remains unclear whether an alteration in the number or distribution of somatostatin (SST) neurones contributes to this change. In the present study, we characterised ... More